Strain Fitness in Escherichia coli BW25113 around b3672

Experiment: 2-nitrobenzenesulfonyl fluoride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntilvB and ivbL are separated by 105 nucleotidesivbL and istR are separated by 129 nucleotidesistR and tisB are separated by 295 nucleotidestisB and emrD are separated by 279 nucleotides b3671: ilvB - acetolactate synthase large subunit (NCBI), at 3,849,119 to 3,850,807 ilvB b3672: ivbL - ilvB operon leader peptide (NCBI), at 3,850,913 to 3,851,011 ivbL b4616: istR - sRNAs IstR-1 and IstR-2, tisB regulators (RefSeq), at 3,851,141 to 3,851,280 istR b4618: tisB - lexA-regulated toxic peptide (RefSeq), at 3,851,576 to 3,851,665 tisB b3673: emrD - 2-module integral membrane pump; multidrug resistance (VIMSS), at 3,851,945 to 3,853,129 emrD Position (kb) 3850 3851 3852Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3849.917 kb on + strand, within ilvBat 3849.917 kb on + strand, within ilvBat 3850.001 kb on - strand, within ilvBat 3850.024 kb on - strand, within ilvBat 3850.027 kb on + strand, within ilvBat 3850.027 kb on + strand, within ilvBat 3850.027 kb on + strand, within ilvBat 3850.142 kb on - strand, within ilvBat 3850.142 kb on - strand, within ilvBat 3850.180 kb on + strand, within ilvBat 3850.187 kb on - strand, within ilvBat 3850.187 kb on - strand, within ilvBat 3850.301 kb on + strand, within ilvBat 3850.301 kb on + strand, within ilvBat 3850.301 kb on + strand, within ilvBat 3850.352 kb on + strand, within ilvBat 3850.358 kb on - strand, within ilvBat 3850.395 kb on - strand, within ilvBat 3850.398 kb on + strand, within ilvBat 3850.408 kb on + strand, within ilvBat 3850.460 kb on + strand, within ilvBat 3850.460 kb on - strand, within ilvBat 3850.498 kb on + strand, within ilvBat 3850.498 kb on + strand, within ilvBat 3850.537 kb on + strand, within ilvBat 3850.556 kb on + strand, within ilvBat 3850.556 kb on + strand, within ilvBat 3850.561 kb on + strand, within ilvBat 3850.561 kb on + strand, within ilvBat 3850.566 kb on + strand, within ilvBat 3850.627 kb on + strand, within ilvBat 3850.635 kb on - strand, within ilvBat 3850.661 kb on + strandat 3850.674 kb on - strandat 3850.674 kb on - strandat 3850.674 kb on - strandat 3850.674 kb on - strandat 3850.690 kb on + strandat 3850.690 kb on + strandat 3850.698 kb on - strandat 3850.709 kb on + strandat 3850.709 kb on + strandat 3850.775 kb on - strandat 3850.791 kb on + strandat 3850.803 kb on + strandat 3850.805 kb on - strandat 3850.805 kb on - strandat 3850.817 kb on - strandat 3850.898 kb on + strandat 3850.901 kb on - strandat 3850.903 kb on + strandat 3850.903 kb on + strandat 3850.911 kb on + strandat 3850.919 kb on - strandat 3850.919 kb on - strandat 3850.939 kb on - strand, within ivbLat 3850.965 kb on - strand, within ivbLat 3850.976 kb on + strand, within ivbLat 3850.976 kb on + strand, within ivbLat 3850.996 kb on + strand, within ivbLat 3850.996 kb on + strand, within ivbLat 3851.001 kb on + strand, within ivbLat 3851.001 kb on + strand, within ivbLat 3851.010 kb on + strandat 3851.010 kb on + strandat 3851.369 kb on + strandat 3851.369 kb on + strandat 3851.379 kb on - strandat 3851.379 kb on - strandat 3851.446 kb on - strandat 3851.446 kb on - strandat 3851.464 kb on - strandat 3851.469 kb on - strandat 3851.469 kb on - strandat 3851.469 kb on - strandat 3851.491 kb on + strandat 3851.493 kb on + strandat 3851.499 kb on - strandat 3851.514 kb on - strandat 3851.545 kb on + strandat 3851.545 kb on + strandat 3851.553 kb on + strandat 3851.592 kb on + strand, within tisBat 3851.605 kb on + strand, within tisBat 3851.627 kb on - strand, within tisBat 3851.644 kb on - strand, within tisBat 3851.709 kb on + strandat 3851.733 kb on + strandat 3851.733 kb on + strandat 3851.772 kb on - strandat 3851.849 kb on - strandat 3851.851 kb on - strandat 3851.977 kb on - strandat 3851.978 kb on + strandat 3851.978 kb on + strandat 3851.986 kb on - strandat 3851.990 kb on + strandat 3851.990 kb on + strandat 3851.997 kb on - strandat 3852.000 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-nitrobenzenesulfonyl fluoride
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3,849,917 + ilvB b3671 0.47 +0.4
3,849,917 + ilvB b3671 0.47 +0.4
3,850,001 - ilvB b3671 0.52 +0.4
3,850,024 - ilvB b3671 0.54 +0.4
3,850,027 + ilvB b3671 0.54 +0.6
3,850,027 + ilvB b3671 0.54 -1.2
3,850,027 + ilvB b3671 0.54 -0.2
3,850,142 - ilvB b3671 0.61 -0.6
3,850,142 - ilvB b3671 0.61 +1.2
3,850,180 + ilvB b3671 0.63 +0.8
3,850,187 - ilvB b3671 0.63 -0.4
3,850,187 - ilvB b3671 0.63 +0.6
3,850,301 + ilvB b3671 0.70 +0.7
3,850,301 + ilvB b3671 0.70 +0.8
3,850,301 + ilvB b3671 0.70 +1.0
3,850,352 + ilvB b3671 0.73 +0.9
3,850,358 - ilvB b3671 0.73 +0.4
3,850,395 - ilvB b3671 0.76 +0.0
3,850,398 + ilvB b3671 0.76 -0.0
3,850,408 + ilvB b3671 0.76 +1.0
3,850,460 + ilvB b3671 0.79 -0.6
3,850,460 - ilvB b3671 0.79 -0.0
3,850,498 + ilvB b3671 0.82 -1.1
3,850,498 + ilvB b3671 0.82 -0.6
3,850,537 + ilvB b3671 0.84 +0.6
3,850,556 + ilvB b3671 0.85 +0.0
3,850,556 + ilvB b3671 0.85 +1.7
3,850,561 + ilvB b3671 0.85 +1.4
3,850,561 + ilvB b3671 0.85 +0.8
3,850,566 + ilvB b3671 0.86 -0.1
3,850,627 + ilvB b3671 0.89 +2.2
3,850,635 - ilvB b3671 0.90 -0.2
3,850,661 + +0.4
3,850,674 - +0.7
3,850,674 - +0.0
3,850,674 - +1.9
3,850,674 - +2.9
3,850,690 + +0.2
3,850,690 + +0.5
3,850,698 - +0.6
3,850,709 + -2.0
3,850,709 + -0.2
3,850,775 - +1.2
3,850,791 + +1.5
3,850,803 + +0.6
3,850,805 - +1.0
3,850,805 - -0.1
3,850,817 - -0.6
3,850,898 + -0.4
3,850,901 - +0.5
3,850,903 + -0.8
3,850,903 + +0.7
3,850,911 + +0.0
3,850,919 - +1.7
3,850,919 - +0.1
3,850,939 - ivbL b3672 0.26 +0.7
3,850,965 - ivbL b3672 0.53 +1.4
3,850,976 + ivbL b3672 0.64 +3.5
3,850,976 + ivbL b3672 0.64 +0.4
3,850,996 + ivbL b3672 0.84 +0.4
3,850,996 + ivbL b3672 0.84 -0.1
3,851,001 + ivbL b3672 0.89 +0.1
3,851,001 + ivbL b3672 0.89 -0.2
3,851,010 + +0.4
3,851,010 + -0.9
3,851,369 + -0.2
3,851,369 + -0.0
3,851,379 - +0.5
3,851,379 - -0.9
3,851,446 - -0.6
3,851,446 - -0.1
3,851,464 - -0.2
3,851,469 - +0.4
3,851,469 - +1.4
3,851,469 - -1.6
3,851,491 + -0.2
3,851,493 + -0.2
3,851,499 - -0.4
3,851,514 - -1.7
3,851,545 + -0.1
3,851,545 + +0.1
3,851,553 + -0.9
3,851,592 + tisB b4618 0.18 +0.6
3,851,605 + tisB b4618 0.32 -0.5
3,851,627 - tisB b4618 0.57 -0.5
3,851,644 - tisB b4618 0.76 -0.2
3,851,709 + +0.1
3,851,733 + +0.3
3,851,733 + -0.9
3,851,772 - -0.2
3,851,849 - +1.4
3,851,851 - -0.4
3,851,977 - +1.9
3,851,978 + -0.5
3,851,978 + +0.7
3,851,986 - +2.5
3,851,990 + +0.2
3,851,990 + +0.4
3,851,997 - +0.2
3,852,000 - +0.5

Or see this region's nucleotide sequence