Strain Fitness in Escherichia coli BW25113 around b2446

Experiment: 2-nitrobenzenesulfonyl fluoride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyffM and yffN are separated by 11 nucleotidesyffN and yffO are separated by 117 nucleotidesyffO and yffP overlap by 4 nucleotides b2444: yffM - CPZ-55 prophage; predicted protein (NCBI), at 2,559,390 to 2,559,635 yffM b2445: yffN - CPZ-55 prophage; predicted protein (RefSeq), at 2,559,647 to 2,560,015 yffN b2446: yffO - CPZ-55 prophage; predicted protein (NCBI), at 2,560,133 to 2,560,549 yffO b2447: yffP - CPZ-55 prophage; predicted protein (NCBI), at 2,560,546 to 2,561,139 yffP Position (kb) 2560 2561Strain fitness (log2 ratio) -2 -1 0 1 2at 2559.256 kb on + strandat 2559.410 kb on - strandat 2559.410 kb on - strandat 2559.536 kb on - strand, within yffMat 2559.536 kb on - strand, within yffMat 2559.699 kb on + strand, within yffNat 2559.794 kb on - strand, within yffNat 2559.794 kb on - strand, within yffNat 2559.897 kb on + strand, within yffNat 2559.897 kb on + strand, within yffNat 2559.915 kb on - strand, within yffNat 2559.941 kb on - strand, within yffNat 2560.369 kb on - strand, within yffOat 2560.487 kb on + strand, within yffOat 2560.549 kb on + strandat 2560.616 kb on - strand, within yffPat 2560.683 kb on + strand, within yffPat 2560.683 kb on + strand, within yffPat 2560.876 kb on - strand, within yffPat 2560.889 kb on - strand, within yffPat 2561.041 kb on - strand, within yffPat 2561.297 kb on - strandat 2561.297 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-nitrobenzenesulfonyl fluoride
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2,559,256 + +0.4
2,559,410 - -0.1
2,559,410 - +2.5
2,559,536 - yffM b2444 0.59 +0.9
2,559,536 - yffM b2444 0.59 +2.3
2,559,699 + yffN b2445 0.14 -2.3
2,559,794 - yffN b2445 0.40 -1.1
2,559,794 - yffN b2445 0.40 -0.6
2,559,897 + yffN b2445 0.68 +0.5
2,559,897 + yffN b2445 0.68 -1.0
2,559,915 - yffN b2445 0.73 +1.7
2,559,941 - yffN b2445 0.80 -1.1
2,560,369 - yffO b2446 0.57 +2.1
2,560,487 + yffO b2446 0.85 +0.8
2,560,549 + +2.0
2,560,616 - yffP b2447 0.12 +0.2
2,560,683 + yffP b2447 0.23 +0.1
2,560,683 + yffP b2447 0.23 +0.7
2,560,876 - yffP b2447 0.56 -0.9
2,560,889 - yffP b2447 0.58 -0.3
2,561,041 - yffP b2447 0.83 -0.9
2,561,297 - -0.1
2,561,297 - -0.1

Or see this region's nucleotide sequence