Strain Fitness in Escherichia coli BW25113 around b2215

Experiment: 2-nitrobenzenesulfonyl fluoride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyojL and ompC are separated by 111 nucleotidesompC and micF are separated by 334 nucleotidesmicF and rcsD are separated by 311 nucleotides b2214: yojL - predicted thiamine biosynthesis lipoprotein (NCBI), at 2,308,501 to 2,309,556 yojL b2215: ompC - outer membrane porin protein C (NCBI), at 2,309,668 to 2,310,771 ompC b4439: micF - regulatory sRNA (NCBI), at 2,311,106 to 2,311,198 micF b2216: rcsD - phosphotransfer intermediate protein in two-component regulatory system with RcsBC (NCBI), at 2,311,510 to 2,314,182 rcsD Position (kb) 2309 2310 2311Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2308.745 kb on - strand, within yojLat 2308.784 kb on - strand, within yojLat 2308.796 kb on - strand, within yojLat 2308.916 kb on + strand, within yojLat 2308.916 kb on + strand, within yojLat 2308.964 kb on - strand, within yojLat 2308.964 kb on - strand, within yojLat 2309.070 kb on - strand, within yojLat 2309.100 kb on + strand, within yojLat 2309.123 kb on - strand, within yojLat 2309.286 kb on - strand, within yojLat 2309.286 kb on - strand, within yojLat 2309.711 kb on - strandat 2309.963 kb on + strand, within ompCat 2309.981 kb on + strand, within ompCat 2310.013 kb on + strand, within ompCat 2310.044 kb on + strand, within ompCat 2310.052 kb on - strand, within ompCat 2310.052 kb on - strandat 2310.081 kb on - strand, within ompCat 2310.091 kb on - strand, within ompCat 2310.155 kb on + strand, within ompCat 2310.192 kb on + strand, within ompCat 2310.277 kb on + strand, within ompCat 2310.340 kb on + strand, within ompCat 2310.362 kb on - strand, within ompCat 2310.362 kb on - strand, within ompCat 2310.362 kb on - strand, within ompCat 2310.442 kb on + strand, within ompCat 2310.442 kb on + strand, within ompCat 2310.490 kb on + strand, within ompCat 2310.531 kb on + strand, within ompCat 2310.532 kb on + strand, within ompCat 2310.538 kb on - strand, within ompCat 2310.538 kb on - strand, within ompCat 2310.562 kb on - strand, within ompCat 2310.599 kb on + strand, within ompCat 2310.601 kb on + strand, within ompCat 2310.602 kb on + strand, within ompCat 2310.602 kb on + strand, within ompCat 2310.602 kb on + strand, within ompCat 2310.602 kb on + strand, within ompCat 2310.607 kb on + strand, within ompCat 2310.607 kb on + strand, within ompCat 2310.608 kb on - strand, within ompCat 2310.610 kb on - strand, within ompCat 2310.615 kb on - strand, within ompCat 2310.629 kb on - strand, within ompCat 2310.672 kb on + strandat 2310.680 kb on - strandat 2310.680 kb on - strandat 2310.700 kb on + strandat 2310.700 kb on + strandat 2310.721 kb on + strandat 2310.725 kb on + strandat 2310.734 kb on - strandat 2310.736 kb on + strandat 2310.737 kb on + strandat 2310.743 kb on - strandat 2310.746 kb on + strandat 2310.746 kb on + strandat 2310.748 kb on + strandat 2310.765 kb on - strandat 2310.765 kb on - strandat 2310.787 kb on - strandat 2310.787 kb on - strandat 2310.821 kb on + strandat 2310.824 kb on + strandat 2310.824 kb on - strandat 2310.824 kb on - strandat 2310.824 kb on - strandat 2310.824 kb on - strandat 2310.826 kb on + strandat 2310.829 kb on - strandat 2310.834 kb on + strandat 2310.835 kb on + strandat 2310.835 kb on + strandat 2310.842 kb on - strandat 2310.843 kb on - strandat 2310.883 kb on - strandat 2310.888 kb on - strandat 2310.889 kb on + strandat 2310.928 kb on + strandat 2311.091 kb on + strandat 2311.204 kb on + strandat 2311.233 kb on + strandat 2311.245 kb on + strandat 2311.250 kb on - strandat 2311.342 kb on - strandat 2311.347 kb on + strandat 2311.347 kb on + strandat 2311.347 kb on + strandat 2311.399 kb on - strandat 2311.409 kb on - strandat 2311.421 kb on + strandat 2311.428 kb on - strandat 2311.428 kb on - strandat 2311.434 kb on - strandat 2311.435 kb on - strandat 2311.438 kb on - strandat 2311.438 kb on - strandat 2311.453 kb on - strandat 2311.453 kb on - strandat 2311.453 kb on - strandat 2311.457 kb on + strandat 2311.457 kb on + strandat 2311.458 kb on + strandat 2311.459 kb on + strandat 2311.459 kb on + strandat 2311.459 kb on + strandat 2311.467 kb on - strandat 2311.467 kb on - strandat 2311.467 kb on - strandat 2311.467 kb on - strandat 2311.467 kb on - strandat 2311.467 kb on - strandat 2311.467 kb on - strandat 2311.467 kb on - strandat 2311.478 kb on - strandat 2311.552 kb on + strandat 2311.552 kb on + strandat 2311.586 kb on + strandat 2311.613 kb on + strandat 2311.613 kb on + strandat 2311.613 kb on - strandat 2311.687 kb on + strandat 2311.687 kb on + strandat 2311.737 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-nitrobenzenesulfonyl fluoride
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2,308,745 - yojL b2214 0.23 -0.8
2,308,784 - yojL b2214 0.27 -2.1
2,308,796 - yojL b2214 0.28 -2.4
2,308,916 + yojL b2214 0.39 -0.9
2,308,916 + yojL b2214 0.39 -1.5
2,308,964 - yojL b2214 0.44 -1.0
2,308,964 - yojL b2214 0.44 -2.0
2,309,070 - yojL b2214 0.54 -0.3
2,309,100 + yojL b2214 0.57 -2.1
2,309,123 - yojL b2214 0.59 -1.9
2,309,286 - yojL b2214 0.74 -2.9
2,309,286 - yojL b2214 0.74 +0.7
2,309,711 - +1.0
2,309,963 + ompC b2215 0.27 +1.4
2,309,981 + ompC b2215 0.28 -0.3
2,310,013 + ompC b2215 0.31 +1.3
2,310,044 + ompC b2215 0.34 +1.9
2,310,052 - ompC b2215 0.35 -0.3
2,310,052 - -0.3
2,310,081 - ompC b2215 0.37 +0.9
2,310,091 - ompC b2215 0.38 +1.0
2,310,155 + ompC b2215 0.44 -1.3
2,310,192 + ompC b2215 0.47 -0.3
2,310,277 + ompC b2215 0.55 +0.7
2,310,340 + ompC b2215 0.61 +2.1
2,310,362 - ompC b2215 0.63 +1.6
2,310,362 - ompC b2215 0.63 +1.2
2,310,362 - ompC b2215 0.63 +2.3
2,310,442 + ompC b2215 0.70 +2.0
2,310,442 + ompC b2215 0.70 +0.9
2,310,490 + ompC b2215 0.74 +0.4
2,310,531 + ompC b2215 0.78 +1.8
2,310,532 + ompC b2215 0.78 +1.1
2,310,538 - ompC b2215 0.79 +2.7
2,310,538 - ompC b2215 0.79 +2.6
2,310,562 - ompC b2215 0.81 +0.9
2,310,599 + ompC b2215 0.84 +2.8
2,310,601 + ompC b2215 0.85 +1.0
2,310,602 + ompC b2215 0.85 +2.5
2,310,602 + ompC b2215 0.85 +1.7
2,310,602 + ompC b2215 0.85 +1.7
2,310,602 + ompC b2215 0.85 +0.7
2,310,607 + ompC b2215 0.85 +1.3
2,310,607 + ompC b2215 0.85 -0.3
2,310,608 - ompC b2215 0.85 +1.2
2,310,610 - ompC b2215 0.85 +1.6
2,310,615 - ompC b2215 0.86 +1.5
2,310,629 - ompC b2215 0.87 +1.4
2,310,672 + +1.9
2,310,680 - +0.7
2,310,680 - +2.5
2,310,700 + +2.1
2,310,700 + +2.5
2,310,721 + -0.0
2,310,725 + -0.3
2,310,734 - +1.3
2,310,736 + -0.3
2,310,737 + +1.5
2,310,743 - +0.7
2,310,746 + +1.5
2,310,746 + +0.3
2,310,748 + +1.9
2,310,765 - +1.2
2,310,765 - +2.3
2,310,787 - +0.3
2,310,787 - +1.6
2,310,821 + +1.9
2,310,824 + +1.7
2,310,824 - +0.9
2,310,824 - -1.3
2,310,824 - +1.0
2,310,824 - -0.1
2,310,826 + +0.5
2,310,829 - -0.0
2,310,834 + +1.3
2,310,835 + +1.7
2,310,835 + -0.3
2,310,842 - +0.3
2,310,843 - -0.0
2,310,883 - +1.2
2,310,888 - +1.5
2,310,889 + +2.4
2,310,928 + +1.1
2,311,091 + -0.5
2,311,204 + -0.9
2,311,233 + -1.3
2,311,245 + -1.9
2,311,250 - -0.0
2,311,342 - +0.5
2,311,347 + -0.3
2,311,347 + +1.3
2,311,347 + +0.7
2,311,399 - -0.5
2,311,409 - -0.5
2,311,421 + +0.3
2,311,428 - -0.3
2,311,428 - +0.4
2,311,434 - -0.1
2,311,435 - +0.6
2,311,438 - +2.1
2,311,438 - -0.1
2,311,453 - -0.0
2,311,453 - +0.3
2,311,453 - -0.3
2,311,457 + +1.3
2,311,457 + +1.2
2,311,458 + -0.7
2,311,459 + -0.3
2,311,459 + -0.3
2,311,459 + -0.9
2,311,467 - -0.3
2,311,467 - -0.9
2,311,467 - -0.4
2,311,467 - +1.5
2,311,467 - +0.7
2,311,467 - -0.3
2,311,467 - +0.7
2,311,467 - -0.3
2,311,478 - +0.2
2,311,552 + +0.7
2,311,552 + +0.7
2,311,586 + +1.1
2,311,613 + -0.9
2,311,613 + -0.7
2,311,613 - +1.0
2,311,687 + +0.1
2,311,687 + -0.0
2,311,737 - +1.4

Or see this region's nucleotide sequence