Strain Fitness in Escherichia coli BW25113 around b1569

Experiment: 2-nitrobenzenesulfonyl fluoride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflxA and intK are separated by 436 nucleotidesintK and ydfX are separated by 1 nucleotidesydfX and dicC overlap by 17 nucleotidesdicC and dicA are separated by 83 nucleotidesdicA and ydfA are separated by 166 nucleotidesydfA and ydfB are separated by 1 nucleotidesydfB and ydfC are separated by 29 nucleotides b1566: flxA - Qin prophage; predicted protein (NCBI), at 1,644,429 to 1,644,761 flxA b1567: intK - Qin prophage; predicted protein (RefSeq), at 1,645,198 to 1,645,380 intK b1568: ydfX - Qin prophage; predicted protein (RefSeq), at 1,645,382 to 1,645,660 ydfX b1569: dicC - Qin prophage; DNA-binding transcriptional regulator for DicB (NCBI), at 1,645,644 to 1,645,874 dicC b1570: dicA - Qin prophage; predicted regulator for DicB (NCBI), at 1,645,958 to 1,646,365 dicA b1571: ydfA - Qin prophage; predicted protein (NCBI), at 1,646,532 to 1,646,687 ydfA b1572: ydfB - orf, hypothetical protein (VIMSS), at 1,646,689 to 1,646,817 ydfB b1573: ydfC - Qin prophage; predicted protein (RefSeq), at 1,646,847 to 1,647,065 ydfC Position (kb) 1645 1646Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1644.647 kb on + strand, within flxAat 1644.647 kb on + strand, within flxAat 1644.656 kb on + strand, within flxAat 1644.690 kb on + strand, within flxAat 1644.756 kb on + strandat 1644.756 kb on + strandat 1644.758 kb on + strandat 1644.774 kb on + strandat 1644.787 kb on - strandat 1644.792 kb on - strandat 1644.809 kb on - strandat 1644.809 kb on - strandat 1644.870 kb on - strandat 1644.870 kb on - strandat 1644.870 kb on - strandat 1644.890 kb on - strandat 1644.918 kb on - strandat 1644.986 kb on - strandat 1645.019 kb on + strandat 1645.019 kb on + strandat 1645.027 kb on - strandat 1645.027 kb on - strandat 1645.038 kb on - strandat 1645.060 kb on + strandat 1645.062 kb on + strandat 1645.087 kb on + strandat 1645.116 kb on - strandat 1645.144 kb on + strandat 1645.145 kb on + strandat 1645.174 kb on - strandat 1645.176 kb on - strandat 1645.280 kb on + strand, within intKat 1645.288 kb on - strand, within intKat 1645.295 kb on + strand, within intKat 1645.408 kb on - strandat 1645.408 kb on - strandat 1645.546 kb on + strand, within ydfXat 1645.640 kb on + strandat 1645.640 kb on + strandat 1645.650 kb on - strandat 1645.725 kb on - strand, within dicCat 1645.725 kb on - strand, within dicCat 1645.940 kb on + strandat 1646.751 kb on - strand, within ydfB

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-nitrobenzenesulfonyl fluoride
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1,644,647 + flxA b1566 0.65 -0.6
1,644,647 + flxA b1566 0.65 -1.6
1,644,656 + flxA b1566 0.68 +0.4
1,644,690 + flxA b1566 0.78 -0.4
1,644,756 + +0.4
1,644,756 + +1.7
1,644,758 + +0.1
1,644,774 + -2.9
1,644,787 - -0.6
1,644,792 - -0.6
1,644,809 - +1.0
1,644,809 - -0.0
1,644,870 - +0.4
1,644,870 - -0.0
1,644,870 - -0.2
1,644,890 - -0.4
1,644,918 - +0.8
1,644,986 - -0.2
1,645,019 + -1.6
1,645,019 + -0.6
1,645,027 - +0.4
1,645,027 - +0.0
1,645,038 - +0.8
1,645,060 + +1.0
1,645,062 + -0.6
1,645,087 + +0.4
1,645,116 - +1.4
1,645,144 + -1.2
1,645,145 + -0.6
1,645,174 - +0.1
1,645,176 - -0.6
1,645,280 + intK b1567 0.45 +0.4
1,645,288 - intK b1567 0.49 +0.8
1,645,295 + intK b1567 0.53 +1.0
1,645,408 - -1.1
1,645,408 - -0.2
1,645,546 + ydfX b1568 0.59 -1.2
1,645,640 + -1.6
1,645,640 + +0.4
1,645,650 - +1.8
1,645,725 - dicC b1569 0.35 +0.8
1,645,725 - dicC b1569 0.35 +0.9
1,645,940 + -0.0
1,646,751 - ydfB b1572 0.48 +0.1

Or see this region's nucleotide sequence