Strain Fitness in Escherichia coli BW25113 around b1253

Experiment: 2-nitrobenzenesulfonyl fluoride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyciI and tonB are separated by 223 nucleotidestonB and yciA are separated by 39 nucleotidesyciA and yciB are separated by 104 nucleotidesyciB and yciC are separated by 29 nucleotides b1251: yciI - orf, hypothetical protein (VIMSS), at 1,308,593 to 1,308,889 yciI b1252: tonB - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 1,309,113 to 1,309,832 tonB b1253: yciA - predicted hydrolase (NCBI), at 1,309,872 to 1,310,270 yciA b1254: yciB - intracellular septation protein A (NCBI), at 1,310,375 to 1,310,914 yciB b1255: yciC - hypothetical protein (NCBI), at 1,310,944 to 1,311,687 yciC Position (kb) 1309 1310 1311Strain fitness (log2 ratio) -2 -1 0 1 2at 1309.193 kb on + strand, within tonBat 1309.201 kb on - strand, within tonBat 1309.201 kb on - strand, within tonBat 1309.201 kb on - strand, within tonBat 1309.474 kb on + strand, within tonBat 1309.533 kb on - strand, within tonBat 1309.533 kb on - strand, within tonBat 1309.610 kb on + strand, within tonBat 1309.705 kb on - strand, within tonBat 1309.705 kb on - strand, within tonBat 1310.036 kb on + strand, within yciAat 1310.036 kb on + strand, within yciAat 1310.046 kb on + strand, within yciAat 1310.061 kb on + strand, within yciAat 1310.141 kb on - strand, within yciAat 1310.410 kb on + strandat 1310.416 kb on - strandat 1310.650 kb on - strand, within yciBat 1310.809 kb on - strand, within yciBat 1311.105 kb on + strand, within yciCat 1311.105 kb on + strand, within yciCat 1311.187 kb on - strand, within yciCat 1311.189 kb on + strand, within yciCat 1311.212 kb on - strand, within yciCat 1311.212 kb on - strand, within yciC

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-nitrobenzenesulfonyl fluoride
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1,309,193 + tonB b1252 0.11 -0.9
1,309,201 - tonB b1252 0.12 +0.5
1,309,201 - tonB b1252 0.12 -0.5
1,309,201 - tonB b1252 0.12 -0.5
1,309,474 + tonB b1252 0.50 -0.2
1,309,533 - tonB b1252 0.58 -0.1
1,309,533 - tonB b1252 0.58 +0.2
1,309,610 + tonB b1252 0.69 -2.1
1,309,705 - tonB b1252 0.82 +2.3
1,309,705 - tonB b1252 0.82 -1.5
1,310,036 + yciA b1253 0.41 +1.1
1,310,036 + yciA b1253 0.41 -0.5
1,310,046 + yciA b1253 0.44 +0.5
1,310,061 + yciA b1253 0.47 +1.3
1,310,141 - yciA b1253 0.67 +1.1
1,310,410 + +0.5
1,310,416 - -0.5
1,310,650 - yciB b1254 0.51 -0.5
1,310,809 - yciB b1254 0.80 -2.5
1,311,105 + yciC b1255 0.22 -0.5
1,311,105 + yciC b1255 0.22 +0.1
1,311,187 - yciC b1255 0.33 -1.8
1,311,189 + yciC b1255 0.33 -0.5
1,311,212 - yciC b1255 0.36 -0.0
1,311,212 - yciC b1255 0.36 -0.2

Or see this region's nucleotide sequence