Experiment: 2-nitrobenzenesulfonyl fluoride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybjM and grxA are separated by 29 nucleotides grxA and ybjC are separated by 159 nucleotides ybjC and nfsA overlap by 17 nucleotides nfsA and rimK are separated by 60 nucleotides
b0848: ybjM - predicted inner membrane protein (NCBI), at 889,312 to 889,689
ybjM
b0849: grxA - glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a) (NCBI), at 889,719 to 889,976
grxA
b0850: ybjC - predicted inner membrane protein (NCBI), at 890,136 to 890,423
ybjC
b0851: nfsA - nitroreductase A, NADPH-dependent, FMN-dependent (NCBI), at 890,407 to 891,129
nfsA
b0852: rimK - ribosomal protein S6 modification protein (NCBI), at 891,190 to 892,092
rimK
Position (kb) 890
891
892 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6
7 at 889.460 kb on - strand, within ybjM at 889.482 kb on + strand, within ybjM at 889.482 kb on + strand, within ybjM at 889.482 kb on + strand, within ybjM at 889.482 kb on + strand, within ybjM at 889.538 kb on - strand, within ybjM at 889.538 kb on - strand, within ybjM at 889.586 kb on + strand, within ybjM at 889.586 kb on + strand, within ybjM at 889.594 kb on + strand, within ybjM at 889.604 kb on - strand, within ybjM at 889.604 kb on - strand, within ybjM at 889.650 kb on - strand, within ybjM at 889.934 kb on + strand, within grxA at 889.936 kb on + strand, within grxA at 889.938 kb on + strand, within grxA at 889.944 kb on - strand, within grxA at 889.944 kb on - strand, within grxA at 889.988 kb on + strand at 890.213 kb on + strand, within ybjC at 890.218 kb on + strand, within ybjC at 890.232 kb on - strand, within ybjC at 890.232 kb on - strand, within ybjC at 890.326 kb on + strand, within ybjC at 890.491 kb on - strand, within nfsA at 890.714 kb on - strand, within nfsA at 890.715 kb on - strand, within nfsA at 890.783 kb on + strand, within nfsA at 890.792 kb on - strand, within nfsA at 890.958 kb on + strand, within nfsA at 890.979 kb on + strand, within nfsA at 890.979 kb on + strand, within nfsA at 891.034 kb on + strand, within nfsA at 891.057 kb on - strand at 891.213 kb on + strand at 891.222 kb on + strand at 891.243 kb on + strand at 891.251 kb on + strand at 891.251 kb on + strand at 891.251 kb on + strand at 891.251 kb on + strand at 891.254 kb on + strand at 891.387 kb on - strand, within rimK at 891.387 kb on - strand, within rimK at 891.703 kb on - strand, within rimK at 891.703 kb on - strand, within rimK at 891.766 kb on - strand, within rimK at 891.868 kb on - strand, within rimK at 892.020 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-nitrobenzenesulfonyl fluoride remove 889,460 - ybjM b0848 0.39 +0.8 889,482 + ybjM b0848 0.45 -0.2 889,482 + ybjM b0848 0.45 -2.0 889,482 + ybjM b0848 0.45 -0.2 889,482 + ybjM b0848 0.45 +0.6 889,538 - ybjM b0848 0.60 +0.1 889,538 - ybjM b0848 0.60 +0.1 889,586 + ybjM b0848 0.72 +0.3 889,586 + ybjM b0848 0.72 +1.3 889,594 + ybjM b0848 0.75 +0.1 889,604 - ybjM b0848 0.77 -0.8 889,604 - ybjM b0848 0.77 -0.5 889,650 - ybjM b0848 0.89 -0.2 889,934 + grxA b0849 0.83 -1.2 889,936 + grxA b0849 0.84 -2.8 889,938 + grxA b0849 0.85 -2.2 889,944 - grxA b0849 0.87 +2.3 889,944 - grxA b0849 0.87 +1.6 889,988 + -3.8 890,213 + ybjC b0850 0.27 -3.2 890,218 + ybjC b0850 0.28 -3.2 890,232 - ybjC b0850 0.33 +4.7 890,232 - ybjC b0850 0.33 +5.7 890,326 + ybjC b0850 0.66 -1.8 890,491 - nfsA b0851 0.12 +6.1 890,714 - nfsA b0851 0.42 +6.9 890,715 - nfsA b0851 0.43 +4.7 890,783 + nfsA b0851 0.52 +6.6 890,792 - nfsA b0851 0.53 +5.7 890,958 + nfsA b0851 0.76 +6.7 890,979 + nfsA b0851 0.79 +5.6 890,979 + nfsA b0851 0.79 +6.3 891,034 + nfsA b0851 0.87 +6.4 891,057 - +6.4 891,213 + +3.1 891,222 + +3.8 891,243 + +2.8 891,251 + +2.8 891,251 + +3.3 891,251 + +1.8 891,251 + +2.3 891,254 + +1.6 891,387 - rimK b0852 0.22 +4.5 891,387 - rimK b0852 0.22 +3.3 891,703 - rimK b0852 0.57 +2.1 891,703 - rimK b0852 0.57 +1.2 891,766 - rimK b0852 0.64 +0.6 891,868 - rimK b0852 0.75 +1.4 892,020 - +0.7
Or see this region's nucleotide sequence