Experiment: 2-nitrobenzenesulfonyl fluoride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yagU and ykgJ are separated by 247 nucleotides ykgJ and yagV are separated by 312 nucleotides yagV and yagW overlap by 32 nucleotides
b0287: yagU - conserved inner membrane protein (NCBI), at 302,215 to 302,829
yagU
b0288: ykgJ - predicted ferredoxin (NCBI), at 303,077 to 303,406
ykgJ
b0289: yagV - orf, hypothetical protein (VIMSS), at 303,719 to 304,429
yagV
b0290: yagW - predicted receptor (NCBI), at 304,398 to 306,041
yagW
Position (kb) 303
304
305 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 302.780 kb on + strand at 302.780 kb on + strand at 302.782 kb on + strand at 302.805 kb on - strand at 302.809 kb on + strand at 302.809 kb on + strand at 302.810 kb on + strand at 302.810 kb on + strand at 302.810 kb on + strand at 302.815 kb on + strand at 302.815 kb on + strand at 302.830 kb on + strand at 302.908 kb on + strand at 302.916 kb on - strand at 302.919 kb on + strand at 302.919 kb on + strand at 302.920 kb on + strand at 302.920 kb on - strand at 302.922 kb on + strand at 302.925 kb on - strand at 302.928 kb on - strand at 302.928 kb on - strand at 302.928 kb on - strand at 302.928 kb on - strand at 302.928 kb on - strand at 302.932 kb on + strand at 302.936 kb on - strand at 302.940 kb on - strand at 302.941 kb on - strand at 302.943 kb on + strand at 302.943 kb on + strand at 302.950 kb on + strand at 302.951 kb on - strand at 302.951 kb on - strand at 302.961 kb on + strand at 302.961 kb on + strand at 302.961 kb on + strand at 302.966 kb on + strand at 302.966 kb on + strand at 302.966 kb on + strand at 302.966 kb on - strand at 302.966 kb on - strand at 302.967 kb on - strand at 302.967 kb on - strand at 302.967 kb on - strand at 302.969 kb on - strand at 302.974 kb on - strand at 302.974 kb on - strand at 302.974 kb on - strand at 302.999 kb on + strand at 303.001 kb on + strand at 303.023 kb on - strand at 303.023 kb on - strand at 303.049 kb on - strand at 303.085 kb on - strand at 303.085 kb on - strand at 303.088 kb on - strand at 303.092 kb on - strand at 303.093 kb on - strand at 303.104 kb on - strand at 303.104 kb on - strand at 303.104 kb on - strand at 303.104 kb on - strand at 303.106 kb on + strand at 303.113 kb on + strand, within ykgJ at 303.122 kb on - strand, within ykgJ at 303.122 kb on - strand, within ykgJ at 303.122 kb on - strand, within ykgJ at 303.123 kb on + strand, within ykgJ at 303.123 kb on + strand, within ykgJ at 303.129 kb on - strand, within ykgJ at 303.129 kb on - strand, within ykgJ at 303.132 kb on + strand, within ykgJ at 303.132 kb on + strand, within ykgJ at 303.132 kb on + strand, within ykgJ at 303.140 kb on - strand, within ykgJ at 303.140 kb on - strand, within ykgJ at 303.140 kb on - strand, within ykgJ at 303.140 kb on - strand, within ykgJ at 303.149 kb on - strand, within ykgJ at 303.149 kb on - strand, within ykgJ at 303.150 kb on - strand, within ykgJ at 303.165 kb on - strand, within ykgJ at 303.172 kb on - strand, within ykgJ at 303.172 kb on - strand, within ykgJ at 303.172 kb on - strand, within ykgJ at 303.182 kb on + strand, within ykgJ at 303.182 kb on + strand, within ykgJ at 303.190 kb on - strand, within ykgJ at 303.190 kb on - strand, within ykgJ at 303.191 kb on - strand, within ykgJ at 303.191 kb on - strand, within ykgJ at 303.197 kb on - strand, within ykgJ at 303.224 kb on - strand, within ykgJ at 303.228 kb on - strand, within ykgJ at 303.228 kb on - strand, within ykgJ at 303.234 kb on - strand, within ykgJ at 303.250 kb on + strand, within ykgJ at 303.250 kb on + strand, within ykgJ at 303.252 kb on - strand, within ykgJ at 303.258 kb on - strand, within ykgJ at 303.261 kb on + strand, within ykgJ at 303.261 kb on + strand, within ykgJ at 303.294 kb on - strand, within ykgJ at 303.302 kb on + strand, within ykgJ at 303.302 kb on + strand, within ykgJ at 303.310 kb on - strand, within ykgJ at 303.310 kb on - strand, within ykgJ at 303.315 kb on - strand, within ykgJ at 303.375 kb on - strand at 303.377 kb on + strand at 303.380 kb on + strand at 303.391 kb on + strand at 303.395 kb on - strand at 303.399 kb on - strand at 303.406 kb on + strand at 303.424 kb on - strand at 303.436 kb on + strand at 303.436 kb on + strand at 303.454 kb on - strand at 303.454 kb on - strand at 303.463 kb on - strand at 303.592 kb on + strand at 303.592 kb on + strand at 303.599 kb on + strand at 303.599 kb on + strand at 303.616 kb on + strand at 303.724 kb on - strand at 303.724 kb on - strand at 303.803 kb on - strand, within yagV at 303.814 kb on - strand, within yagV at 303.814 kb on - strand, within yagV at 303.824 kb on - strand, within yagV at 303.933 kb on + strand, within yagV at 304.213 kb on + strand, within yagV at 304.308 kb on + strand, within yagV at 304.308 kb on + strand, within yagV at 304.369 kb on + strand at 304.369 kb on + strand at 304.395 kb on + strand at 304.617 kb on - strand, within yagW at 304.695 kb on + strand, within yagW at 304.707 kb on + strand, within yagW at 304.707 kb on + strand, within yagW at 304.717 kb on + strand, within yagW at 304.750 kb on + strand, within yagW at 304.825 kb on + strand, within yagW at 304.825 kb on + strand, within yagW at 304.869 kb on - strand, within yagW at 304.869 kb on - strand, within yagW at 305.031 kb on - strand, within yagW at 305.103 kb on - strand, within yagW at 305.136 kb on + strand, within yagW at 305.148 kb on + strand, within yagW at 305.148 kb on + strand, within yagW at 305.189 kb on + strand, within yagW at 305.189 kb on + strand, within yagW at 305.246 kb on + strand, within yagW
Per-strain Table
Position Strand Gene LocusTag Fraction 2-nitrobenzenesulfonyl fluoride remove 302,780 + -0.3 302,780 + +1.3 302,782 + -0.0 302,805 - -2.0 302,809 + +0.6 302,809 + +1.0 302,810 + +0.9 302,810 + +2.2 302,810 + +0.3 302,815 + +0.2 302,815 + +1.0 302,830 + +0.8 302,908 + -0.0 302,916 - +1.0 302,919 + +1.0 302,919 + -1.0 302,920 + -0.0 302,920 - +1.2 302,922 + +2.6 302,925 - -0.9 302,928 - -0.0 302,928 - +0.7 302,928 - +0.9 302,928 - +1.6 302,928 - +0.8 302,932 + -0.4 302,936 - -1.0 302,940 - +0.6 302,941 - -0.4 302,943 + +2.3 302,943 + +1.0 302,950 + -0.3 302,951 - +0.2 302,951 - -0.0 302,961 + -2.3 302,961 + +2.6 302,961 + -0.6 302,966 + -0.0 302,966 + -0.3 302,966 + +2.0 302,966 - +0.6 302,966 - +1.6 302,967 - -0.0 302,967 - +1.0 302,967 - +0.3 302,969 - +0.1 302,974 - +0.3 302,974 - +0.6 302,974 - -0.3 302,999 + +0.3 303,001 + -0.0 303,023 - -0.0 303,023 - -0.2 303,049 - +0.3 303,085 - -3.0 303,085 - +2.3 303,088 - -0.6 303,092 - +0.6 303,093 - -0.8 303,104 - -0.0 303,104 - +1.0 303,104 - -0.8 303,104 - -1.0 303,106 + +1.0 303,113 + ykgJ b0288 0.11 -1.0 303,122 - ykgJ b0288 0.14 +0.9 303,122 - ykgJ b0288 0.14 -0.0 303,122 - ykgJ b0288 0.14 +1.2 303,123 + ykgJ b0288 0.14 +2.8 303,123 + ykgJ b0288 0.14 +0.1 303,129 - ykgJ b0288 0.16 +1.2 303,129 - ykgJ b0288 0.16 +0.3 303,132 + ykgJ b0288 0.17 +0.6 303,132 + ykgJ b0288 0.17 +1.0 303,132 + ykgJ b0288 0.17 +0.6 303,140 - ykgJ b0288 0.19 -0.0 303,140 - ykgJ b0288 0.19 -0.1 303,140 - ykgJ b0288 0.19 +0.8 303,140 - ykgJ b0288 0.19 +0.7 303,149 - ykgJ b0288 0.22 -0.7 303,149 - ykgJ b0288 0.22 -1.8 303,150 - ykgJ b0288 0.22 -0.5 303,165 - ykgJ b0288 0.27 +0.5 303,172 - ykgJ b0288 0.29 -0.3 303,172 - ykgJ b0288 0.29 -0.0 303,172 - ykgJ b0288 0.29 +1.6 303,182 + ykgJ b0288 0.32 -1.0 303,182 + ykgJ b0288 0.32 -1.6 303,190 - ykgJ b0288 0.34 +0.8 303,190 - ykgJ b0288 0.34 -0.0 303,191 - ykgJ b0288 0.35 -2.0 303,191 - ykgJ b0288 0.35 -0.5 303,197 - ykgJ b0288 0.36 +0.6 303,224 - ykgJ b0288 0.45 +0.8 303,228 - ykgJ b0288 0.46 +2.2 303,228 - ykgJ b0288 0.46 +0.5 303,234 - ykgJ b0288 0.48 -0.3 303,250 + ykgJ b0288 0.52 -1.0 303,250 + ykgJ b0288 0.52 +0.7 303,252 - ykgJ b0288 0.53 +0.2 303,258 - ykgJ b0288 0.55 +0.7 303,261 + ykgJ b0288 0.56 +0.7 303,261 + ykgJ b0288 0.56 -0.0 303,294 - ykgJ b0288 0.66 -0.2 303,302 + ykgJ b0288 0.68 +1.4 303,302 + ykgJ b0288 0.68 +2.3 303,310 - ykgJ b0288 0.71 -0.0 303,310 - ykgJ b0288 0.71 -1.3 303,315 - ykgJ b0288 0.72 +1.9 303,375 - -1.6 303,377 + -1.0 303,380 + -0.5 303,391 + -0.4 303,395 - +0.7 303,399 - -0.2 303,406 + +1.4 303,424 - +0.1 303,436 + -0.8 303,436 + -0.2 303,454 - +0.2 303,454 - -0.3 303,463 - +0.6 303,592 + -1.3 303,592 + +0.3 303,599 + -0.0 303,599 + +0.6 303,616 + -0.7 303,724 - -0.0 303,724 - -0.0 303,803 - yagV b0289 0.12 -0.0 303,814 - yagV b0289 0.13 +1.8 303,814 - yagV b0289 0.13 +0.8 303,824 - yagV b0289 0.15 +1.6 303,933 + yagV b0289 0.30 +1.1 304,213 + yagV b0289 0.69 -1.6 304,308 + yagV b0289 0.83 +0.9 304,308 + yagV b0289 0.83 -1.0 304,369 + +0.9 304,369 + -0.6 304,395 + +0.1 304,617 - yagW b0290 0.13 +1.0 304,695 + yagW b0290 0.18 +0.2 304,707 + yagW b0290 0.19 +0.7 304,707 + yagW b0290 0.19 -2.0 304,717 + yagW b0290 0.19 -0.0 304,750 + yagW b0290 0.21 +0.9 304,825 + yagW b0290 0.26 +0.3 304,825 + yagW b0290 0.26 -0.0 304,869 - yagW b0290 0.29 +0.4 304,869 - yagW b0290 0.29 +1.0 305,031 - yagW b0290 0.39 -0.0 305,103 - yagW b0290 0.43 -1.3 305,136 + yagW b0290 0.45 +0.6 305,148 + yagW b0290 0.46 +1.2 305,148 + yagW b0290 0.46 +1.3 305,189 + yagW b0290 0.48 +1.2 305,189 + yagW b0290 0.48 +0.6 305,246 + yagW b0290 0.52 +0.6
Or see this region's nucleotide sequence