Experiment: 2-nitrobenzenesulfonyl fluoride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ykfI and yafW are separated by 20 nucleotides yafW and ykfH are separated by 18 nucleotides ykfH and ykfG are separated by 8 nucleotides ykfG and yafX are separated by 15 nucleotides yafX and ykfF are separated by 97 nucleotides ykfF and ykfB are separated by 76 nucleotides
b0245: ykfI - CP4-6 prophage; toxin of the YkfI-YafW toxin-antitoxin system (NCBI), at 262,552 to 262,893
ykfI
b0246: yafW - CP4-6 prophage; antitoxin of the YkfI-YafW toxin-antitoxin system (NCBI), at 262,914 to 263,231
yafW
b4504: ykfH - hypothetical protein (NCBI), at 263,250 to 263,471
ykfH
b0247: ykfG - CP4-6 prophage; predicted DNA repair protein (NCBI), at 263,480 to 263,956
ykfG
b0248: yafX - CP4-6 prophage; predicted protein (NCBI), at 263,972 to 264,430
yafX
b0249: ykfF - CP4-6 prophage; predicted protein (NCBI), at 264,528 to 264,767
ykfF
b0250: ykfB - CP4-6 prophage; predicted protein (NCBI), at 264,844 to 265,311
ykfB
Position (kb) 263
264 Strain fitness (log2 ratio) -2
-1
0
1
2 at 262.546 kb on - strand at 262.548 kb on + strand at 262.565 kb on + strand at 262.644 kb on - strand, within ykfI at 262.717 kb on - strand, within ykfI at 262.719 kb on + strand, within ykfI at 262.727 kb on - strand, within ykfI at 262.727 kb on - strand, within ykfI at 262.727 kb on - strand, within ykfI at 262.730 kb on + strand, within ykfI at 262.730 kb on + strand, within ykfI at 262.738 kb on - strand, within ykfI at 262.760 kb on + strand, within ykfI at 262.786 kb on - strand, within ykfI at 262.909 kb on - strand at 262.909 kb on - strand at 262.917 kb on + strand at 262.917 kb on + strand at 262.917 kb on + strand at 262.935 kb on - strand at 262.943 kb on + strand at 262.943 kb on + strand at 262.980 kb on + strand, within yafW at 262.980 kb on + strand, within yafW at 262.994 kb on + strand, within yafW at 262.998 kb on - strand, within yafW at 263.008 kb on - strand, within yafW at 263.008 kb on - strand, within yafW at 263.078 kb on - strand, within yafW at 263.151 kb on - strand, within yafW at 263.169 kb on + strand, within yafW at 263.192 kb on + strand, within yafW at 263.226 kb on + strand at 263.234 kb on - strand at 263.234 kb on - strand at 263.234 kb on - strand at 263.273 kb on + strand, within ykfH at 263.278 kb on + strand, within ykfH at 263.365 kb on - strand, within ykfH at 263.393 kb on - strand, within ykfH at 263.486 kb on - strand at 263.567 kb on + strand, within ykfG at 263.994 kb on + strand at 263.994 kb on + strand at 264.009 kb on - strand at 264.039 kb on - strand, within yafX at 264.039 kb on - strand, within yafX at 264.097 kb on + strand, within yafX at 264.118 kb on + strand, within yafX at 264.232 kb on + strand, within yafX at 264.256 kb on + strand, within yafX at 264.291 kb on + strand, within yafX at 264.426 kb on + strand at 264.483 kb on + strand at 264.539 kb on - strand at 264.539 kb on - strand at 264.542 kb on + strand at 264.550 kb on - strand at 264.554 kb on + strand, within ykfF at 264.674 kb on - strand, within ykfF at 264.701 kb on - strand, within ykfF at 264.752 kb on + strand at 264.752 kb on + strand at 264.953 kb on + strand, within ykfB
Per-strain Table
Position Strand Gene LocusTag Fraction 2-nitrobenzenesulfonyl fluoride remove 262,546 - +0.3 262,548 + +0.3 262,565 + -0.0 262,644 - ykfI b0245 0.27 +0.2 262,717 - ykfI b0245 0.48 +0.8 262,719 + ykfI b0245 0.49 -1.0 262,727 - ykfI b0245 0.51 -0.7 262,727 - ykfI b0245 0.51 +0.3 262,727 - ykfI b0245 0.51 +0.6 262,730 + ykfI b0245 0.52 -1.2 262,730 + ykfI b0245 0.52 +1.0 262,738 - ykfI b0245 0.54 -1.8 262,760 + ykfI b0245 0.61 -0.3 262,786 - ykfI b0245 0.68 +1.4 262,909 - -0.0 262,909 - -0.4 262,917 + +0.6 262,917 + -0.1 262,917 + -0.5 262,935 - -0.3 262,943 + +0.4 262,943 + +1.3 262,980 + yafW b0246 0.21 -0.0 262,980 + yafW b0246 0.21 +1.6 262,994 + yafW b0246 0.25 +0.1 262,998 - yafW b0246 0.26 +0.2 263,008 - yafW b0246 0.30 -0.1 263,008 - yafW b0246 0.30 +0.7 263,078 - yafW b0246 0.52 +0.9 263,151 - yafW b0246 0.75 +0.6 263,169 + yafW b0246 0.80 -0.3 263,192 + yafW b0246 0.87 -1.6 263,226 + +0.1 263,234 - +1.0 263,234 - +1.6 263,234 - -1.0 263,273 + ykfH b4504 0.10 -1.6 263,278 + ykfH b4504 0.13 +0.4 263,365 - ykfH b4504 0.52 +0.7 263,393 - ykfH b4504 0.64 -0.1 263,486 - -0.5 263,567 + ykfG b0247 0.18 +1.7 263,994 + +1.9 263,994 + +1.0 264,009 - -0.1 264,039 - yafX b0248 0.15 -0.2 264,039 - yafX b0248 0.15 +0.3 264,097 + yafX b0248 0.27 +1.1 264,118 + yafX b0248 0.32 -0.7 264,232 + yafX b0248 0.57 -0.0 264,256 + yafX b0248 0.62 +1.0 264,291 + yafX b0248 0.69 +0.6 264,426 + -0.0 264,483 + -0.2 264,539 - +0.5 264,539 - -0.0 264,542 + +0.2 264,550 - -0.4 264,554 + ykfF b0249 0.11 +1.2 264,674 - ykfF b0249 0.61 +0.3 264,701 - ykfF b0249 0.72 -0.3 264,752 + -0.4 264,752 + +0.5 264,953 + ykfB b0250 0.23 +0.3
Or see this region's nucleotide sequence