Experiment: 2-nitrobenzenesulfonyl fluoride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaeI and dapD are separated by 53 nucleotides dapD and glnD are separated by 30 nucleotides glnD and map are separated by 61 nucleotides
b0164: yaeI - orf, hypothetical protein (VIMSS), at 184,257 to 185,069
yaeI
b0166: dapD - 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase (NCBI), at 185,123 to 185,947
dapD
b0167: glnD - PII uridylyl-transferase (NCBI), at 185,978 to 188,650
glnD
b0168: map - methionine aminopeptidase (NCBI), at 188,712 to 189,506
map
Position (kb) 185
186
187
188
189 Strain fitness (log2 ratio) -1
0
1
2 at 184.993 kb on - strand at 184.993 kb on - strand at 185.102 kb on + strand at 185.102 kb on + strand at 185.105 kb on - strand at 185.107 kb on + strand at 185.949 kb on + strand at 185.969 kb on + strand at 185.969 kb on + strand at 185.976 kb on - strand at 187.184 kb on - strand, within glnD at 187.189 kb on - strand, within glnD at 187.234 kb on - strand, within glnD at 187.234 kb on - strand, within glnD at 187.571 kb on - strand, within glnD at 187.605 kb on - strand, within glnD at 187.924 kb on - strand, within glnD at 188.015 kb on - strand, within glnD at 188.031 kb on + strand, within glnD at 188.129 kb on - strand, within glnD at 188.516 kb on + strand at 188.614 kb on - strand at 188.643 kb on + strand at 188.643 kb on + strand at 188.648 kb on + strand at 188.717 kb on - strand at 188.751 kb on - strand at 189.542 kb on + strand at 189.542 kb on + strand at 189.548 kb on - strand at 189.572 kb on - strand at 189.573 kb on - strand at 189.580 kb on + strand at 189.580 kb on + strand at 189.614 kb on + strand at 189.614 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-nitrobenzenesulfonyl fluoride remove 184,993 - -0.4 184,993 - +1.2 185,102 + -0.2 185,102 + +0.6 185,105 - +1.2 185,107 + +0.3 185,949 + -0.1 185,969 + +0.0 185,969 + +0.0 185,976 - -1.0 187,184 - glnD b0167 0.45 +0.0 187,189 - glnD b0167 0.45 -1.0 187,234 - glnD b0167 0.47 +1.0 187,234 - glnD b0167 0.47 -1.0 187,571 - glnD b0167 0.60 +2.0 187,605 - glnD b0167 0.61 +0.6 187,924 - glnD b0167 0.73 +1.0 188,015 - glnD b0167 0.76 +1.5 188,031 + glnD b0167 0.77 +0.9 188,129 - glnD b0167 0.80 +1.0 188,516 + +0.0 188,614 - -0.1 188,643 + +1.2 188,643 + +0.0 188,648 + -0.2 188,717 - +0.1 188,751 - +0.3 189,542 + +2.0 189,542 + +0.4 189,548 - -0.5 189,572 - +1.0 189,573 - +0.4 189,580 + +0.4 189,580 + +0.0 189,614 + +0.0 189,614 + +0.8
Or see this region's nucleotide sequence