Experiment: Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09015 and PS417_09020 are separated by 30 nucleotides PS417_09020 and PS417_09025 are separated by 97 nucleotides PS417_09025 and PS417_09030 overlap by 4 nucleotides
PS417_09015: PS417_09015 - AMP-binding protein, at 1,962,998 to 1,964,647
_09015
PS417_09020: PS417_09020 - esterase, at 1,964,678 to 1,965,121
_09020
PS417_09025: PS417_09025 - alpha/beta hydrolase, at 1,965,219 to 1,966,022
_09025
PS417_09030: PS417_09030 - alpha/beta hydrolase, at 1,966,019 to 1,966,873
_09030
Position (kb)
1964
1965
1966 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1964.000 kb on + strand, within PS417_09015 at 1964.000 kb on + strand, within PS417_09015 at 1964.001 kb on - strand, within PS417_09015 at 1964.154 kb on - strand, within PS417_09015 at 1964.294 kb on + strand, within PS417_09015 at 1964.458 kb on - strand, within PS417_09015 at 1964.588 kb on + strand at 1964.588 kb on + strand at 1964.828 kb on + strand, within PS417_09020 at 1964.891 kb on + strand, within PS417_09020 at 1964.899 kb on + strand, within PS417_09020 at 1964.918 kb on + strand, within PS417_09020 at 1965.153 kb on + strand at 1965.153 kb on + strand at 1965.154 kb on - strand at 1965.154 kb on - strand at 1965.154 kb on - strand at 1965.154 kb on - strand at 1965.271 kb on - strand at 1965.305 kb on + strand, within PS417_09025 at 1965.305 kb on + strand, within PS417_09025 at 1965.306 kb on - strand, within PS417_09025 at 1965.360 kb on - strand, within PS417_09025 at 1965.486 kb on + strand, within PS417_09025 at 1965.646 kb on - strand, within PS417_09025 at 1965.646 kb on - strand, within PS417_09025 at 1965.700 kb on - strand, within PS417_09025 at 1965.700 kb on - strand, within PS417_09025 at 1965.717 kb on + strand, within PS417_09025 at 1965.718 kb on - strand, within PS417_09025 at 1965.775 kb on - strand, within PS417_09025
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB remove 1,964,000 + PS417_09015 0.61 +1.2 1,964,000 + PS417_09015 0.61 +0.2 1,964,001 - PS417_09015 0.61 -0.8 1,964,154 - PS417_09015 0.70 -2.0 1,964,294 + PS417_09015 0.79 +0.8 1,964,458 - PS417_09015 0.88 -0.7 1,964,588 + +0.1 1,964,588 + -0.1 1,964,828 + PS417_09020 0.34 -0.2 1,964,891 + PS417_09020 0.48 -1.0 1,964,899 + PS417_09020 0.50 -1.9 1,964,918 + PS417_09020 0.54 -0.6 1,965,153 + -0.4 1,965,153 + -0.1 1,965,154 - -0.1 1,965,154 - +1.9 1,965,154 - -0.2 1,965,154 - -1.0 1,965,271 - -3.1 1,965,305 + PS417_09025 0.11 -0.3 1,965,305 + PS417_09025 0.11 -0.7 1,965,306 - PS417_09025 0.11 -0.4 1,965,360 - PS417_09025 0.18 +0.1 1,965,486 + PS417_09025 0.33 -0.6 1,965,646 - PS417_09025 0.53 -0.0 1,965,646 - PS417_09025 0.53 +0.1 1,965,700 - PS417_09025 0.60 -0.8 1,965,700 - PS417_09025 0.60 +0.0 1,965,717 + PS417_09025 0.62 +0.5 1,965,718 - PS417_09025 0.62 +0.4 1,965,775 - PS417_09025 0.69 +0.1
Or see this region's nucleotide sequence