Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14520 and PS417_14525 overlap by 4 nucleotides PS417_14525 and PS417_14530 are separated by 178 nucleotides PS417_14530 and PS417_14535 are separated by 138 nucleotides
PS417_14520: PS417_14520 - methyltransferase, at 3,175,309 to 3,175,908
_14520
PS417_14525: PS417_14525 - glycosyl transferase, at 3,175,905 to 3,176,564
_14525
PS417_14530: PS417_14530 - acetylglucosaminylphosphatidylinositol deacetylase, at 3,176,743 to 3,177,504
_14530
PS417_14535: PS417_14535 - alpha/beta hydrolase, at 3,177,643 to 3,178,677
_14535
Position (kb)
3176
3177
3178 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3176.376 kb on + strand, within PS417_14525 at 3176.577 kb on + strand at 3176.578 kb on - strand at 3176.626 kb on + strand at 3176.827 kb on - strand, within PS417_14530 at 3177.274 kb on + strand, within PS417_14530 at 3177.274 kb on + strand, within PS417_14530 at 3177.275 kb on - strand, within PS417_14530 at 3177.516 kb on + strand at 3177.545 kb on + strand at 3177.546 kb on - strand at 3177.556 kb on + strand at 3177.587 kb on + strand at 3177.902 kb on - strand, within PS417_14535 at 3177.902 kb on - strand, within PS417_14535 at 3177.902 kb on - strand, within PS417_14535 at 3177.902 kb on - strand, within PS417_14535
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB remove 3,176,376 + PS417_14525 0.71 -0.2 3,176,577 + +0.2 3,176,578 - +0.1 3,176,626 + -2.3 3,176,827 - PS417_14530 0.11 -0.4 3,177,274 + PS417_14530 0.70 -1.3 3,177,274 + PS417_14530 0.70 -1.4 3,177,275 - PS417_14530 0.70 -0.3 3,177,516 + +0.7 3,177,545 + +0.1 3,177,546 - +0.2 3,177,556 + +0.5 3,177,587 + +0.4 3,177,902 - PS417_14535 0.25 -1.2 3,177,902 - PS417_14535 0.25 -2.8 3,177,902 - PS417_14535 0.25 -0.3 3,177,902 - PS417_14535 0.25 +0.1
Or see this region's nucleotide sequence