Strain Fitness in Escherichia coli BW25113 around b2439

Experiment: .5% DMSO

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfeG and b2438 are separated by 45 nucleotidesb2438 and eutL are separated by 12 nucleotideseutL and eutC are separated by 9 nucleotideseutC and eutB are separated by 20 nucleotides b2437: yfeG - predicted DNA-binding transcriptional regulator (NCBI), at 2,552,152 to 2,553,204 yfeG b2438: b2438 - orf, hypothetical protein (VIMSS), at 2,553,250 to 2,553,750 b2438 b2439: eutL - predicted carboxysome structural protein with predicted role in ethanolamine utilization (NCBI), at 2,553,763 to 2,554,422 eutL b2440: eutC - ethanolamine ammonia-lyase small subunit (NCBI), at 2,554,432 to 2,555,319 eutC b2441: eutB - ethanolamine ammonia-lyase, heavy chain (VIMSS), at 2,555,340 to 2,556,701 eutB Position (kb) 2553 2554 2555Strain fitness (log2 ratio) -2 -1 0 1 2at 2552.790 kb on - strand, within yfeGat 2552.790 kb on - strand, within yfeGat 2552.812 kb on - strand, within yfeGat 2552.812 kb on - strand, within yfeGat 2552.881 kb on + strand, within yfeGat 2552.881 kb on + strand, within yfeGat 2552.917 kb on + strand, within yfeGat 2552.917 kb on + strand, within yfeGat 2552.932 kb on + strand, within yfeGat 2552.992 kb on - strand, within yfeGat 2553.227 kb on + strandat 2553.256 kb on - strandat 2553.292 kb on + strandat 2553.469 kb on - strand, within b2438at 2553.469 kb on - strand, within b2438at 2553.477 kb on + strand, within b2438at 2553.478 kb on + strand, within b2438at 2553.478 kb on + strand, within b2438at 2553.478 kb on + strand, within b2438at 2553.478 kb on + strand, within b2438at 2553.513 kb on - strand, within b2438at 2553.554 kb on - strand, within b2438at 2553.598 kb on - strand, within b2438at 2553.732 kb on + strandat 2553.922 kb on + strand, within eutLat 2554.133 kb on - strand, within eutLat 2554.133 kb on - strand, within eutLat 2554.218 kb on - strand, within eutLat 2554.225 kb on + strand, within eutLat 2554.260 kb on + strand, within eutLat 2554.325 kb on - strand, within eutLat 2554.436 kb on - strandat 2554.533 kb on - strand, within eutCat 2554.718 kb on + strand, within eutCat 2554.720 kb on + strand, within eutCat 2554.723 kb on - strand, within eutCat 2554.723 kb on - strand, within eutCat 2554.757 kb on - strand, within eutCat 2554.805 kb on + strand, within eutCat 2554.883 kb on + strand, within eutCat 2554.901 kb on - strand, within eutCat 2554.901 kb on - strand, within eutCat 2554.970 kb on + strand, within eutCat 2555.001 kb on + strand, within eutCat 2555.038 kb on - strand, within eutCat 2555.038 kb on - strand, within eutCat 2555.097 kb on + strand, within eutCat 2555.097 kb on + strand, within eutCat 2555.176 kb on + strand, within eutCat 2555.270 kb on + strandat 2555.270 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction .5% DMSO
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2,552,790 - yfeG b2437 0.61 -0.7
2,552,790 - yfeG b2437 0.61 -0.2
2,552,812 - yfeG b2437 0.63 +0.2
2,552,812 - yfeG b2437 0.63 +0.1
2,552,881 + yfeG b2437 0.69 +2.2
2,552,881 + yfeG b2437 0.69 +0.4
2,552,917 + yfeG b2437 0.73 +0.5
2,552,917 + yfeG b2437 0.73 +0.3
2,552,932 + yfeG b2437 0.74 +0.3
2,552,992 - yfeG b2437 0.80 -0.7
2,553,227 + -1.2
2,553,256 - +0.5
2,553,292 + -0.2
2,553,469 - b2438 b2438 0.44 -0.6
2,553,469 - b2438 b2438 0.44 +1.8
2,553,477 + b2438 b2438 0.45 -0.3
2,553,478 + b2438 b2438 0.46 -0.3
2,553,478 + b2438 b2438 0.46 +0.2
2,553,478 + b2438 b2438 0.46 +0.1
2,553,478 + b2438 b2438 0.46 -0.7
2,553,513 - b2438 b2438 0.52 -0.7
2,553,554 - b2438 b2438 0.61 -0.6
2,553,598 - b2438 b2438 0.69 +0.2
2,553,732 + +0.1
2,553,922 + eutL b2439 0.24 +0.5
2,554,133 - eutL b2439 0.56 -1.4
2,554,133 - eutL b2439 0.56 +0.2
2,554,218 - eutL b2439 0.69 -1.2
2,554,225 + eutL b2439 0.70 +0.9
2,554,260 + eutL b2439 0.75 +0.4
2,554,325 - eutL b2439 0.85 +1.0
2,554,436 - +0.1
2,554,533 - eutC b2440 0.11 +0.4
2,554,718 + eutC b2440 0.32 -0.5
2,554,720 + eutC b2440 0.32 +0.4
2,554,723 - eutC b2440 0.33 -0.0
2,554,723 - eutC b2440 0.33 -0.5
2,554,757 - eutC b2440 0.37 -0.4
2,554,805 + eutC b2440 0.42 +0.1
2,554,883 + eutC b2440 0.51 -0.5
2,554,901 - eutC b2440 0.53 +0.8
2,554,901 - eutC b2440 0.53 -1.8
2,554,970 + eutC b2440 0.61 -1.2
2,555,001 + eutC b2440 0.64 +0.1
2,555,038 - eutC b2440 0.68 -0.5
2,555,038 - eutC b2440 0.68 -0.4
2,555,097 + eutC b2440 0.75 +0.2
2,555,097 + eutC b2440 0.75 +0.5
2,555,176 + eutC b2440 0.84 -0.0
2,555,270 + -0.6
2,555,270 + +0.8

Or see this region's nucleotide sequence