Experiment: .5% DMSO
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yejF and yejG are separated by 3 nucleotides yejG and bcr are separated by 332 nucleotides bcr and rsuA are separated by 27 nucleotides rsuA and yejH are separated by 148 nucleotides
b2180: yejF - fused predicted oligopeptide transporter subunits of ABC superfamilly: ATP-binding components (NCBI), at 2,274,322 to 2,275,911
yejF
b2181: yejG - hypothetical protein (NCBI), at 2,275,915 to 2,276,259
yejG
b2182: bcr - bicyclomycin/multidrug efflux system (NCBI), at 2,276,592 to 2,277,782
bcr
b2183: rsuA - 16S rRNA pseudouridylate 516 synthase (NCBI), at 2,277,810 to 2,278,505
rsuA
b2184: yejH - DNA damage response helicase (yejH or radD) (from data) , at 2,278,654 to 2,280,414
yejH
Position (kb)
2276
2277
2278 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 2275.673 kb on + strand, within yejF at 2275.814 kb on - strand at 2275.816 kb on - strand at 2275.870 kb on + strand at 2275.870 kb on + strand at 2276.009 kb on + strand, within yejG at 2276.027 kb on - strand, within yejG at 2276.032 kb on + strand, within yejG at 2276.032 kb on + strand, within yejG at 2276.032 kb on + strand, within yejG at 2276.032 kb on - strand, within yejG at 2276.078 kb on + strand, within yejG at 2276.099 kb on + strand, within yejG at 2276.108 kb on - strand, within yejG at 2276.245 kb on - strand at 2276.332 kb on + strand at 2276.374 kb on + strand at 2276.406 kb on - strand at 2276.411 kb on - strand at 2276.411 kb on - strand at 2276.429 kb on - strand at 2276.885 kb on - strand, within bcr at 2276.892 kb on - strand, within bcr at 2276.892 kb on - strand, within bcr at 2276.950 kb on - strand, within bcr at 2276.950 kb on - strand, within bcr at 2277.132 kb on + strand, within bcr at 2277.137 kb on + strand, within bcr at 2277.197 kb on + strand, within bcr at 2277.287 kb on + strand, within bcr at 2277.287 kb on + strand, within bcr at 2277.352 kb on - strand, within bcr at 2277.352 kb on - strand, within bcr at 2277.352 kb on - strand, within bcr at 2277.422 kb on - strand, within bcr at 2277.445 kb on + strand, within bcr at 2277.573 kb on + strand, within bcr at 2277.625 kb on - strand, within bcr at 2277.628 kb on - strand, within bcr at 2277.641 kb on + strand, within bcr at 2277.695 kb on + strand at 2277.695 kb on + strand at 2277.696 kb on + strand at 2277.759 kb on + strand at 2277.767 kb on + strand at 2278.002 kb on - strand, within rsuA at 2278.018 kb on - strand, within rsuA at 2278.126 kb on - strand, within rsuA at 2278.173 kb on + strand, within rsuA at 2278.266 kb on - strand, within rsuA at 2278.285 kb on - strand, within rsuA at 2278.462 kb on - strand at 2278.560 kb on - strand at 2278.678 kb on - strand at 2278.720 kb on + strand at 2278.720 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction .5% DMSO remove 2,275,673 + yejF b2180 0.85 +1.1 2,275,814 - -0.3 2,275,816 - +0.6 2,275,870 + +0.7 2,275,870 + +0.0 2,276,009 + yejG b2181 0.27 +0.5 2,276,027 - yejG b2181 0.32 +0.1 2,276,032 + yejG b2181 0.34 +0.3 2,276,032 + yejG b2181 0.34 -0.3 2,276,032 + yejG b2181 0.34 -0.3 2,276,032 - yejG b2181 0.34 -0.8 2,276,078 + yejG b2181 0.47 -1.3 2,276,099 + yejG b2181 0.53 -0.9 2,276,108 - yejG b2181 0.56 +0.2 2,276,245 - +1.3 2,276,332 + -0.7 2,276,374 + -1.0 2,276,406 - +0.2 2,276,411 - +0.1 2,276,411 - +0.4 2,276,429 - -1.8 2,276,885 - bcr b2182 0.25 -0.3 2,276,892 - bcr b2182 0.25 -0.3 2,276,892 - bcr b2182 0.25 +0.2 2,276,950 - bcr b2182 0.30 +5.6 2,276,950 - bcr b2182 0.30 +4.9 2,277,132 + bcr b2182 0.45 -0.4 2,277,137 + bcr b2182 0.46 -0.5 2,277,197 + bcr b2182 0.51 +0.5 2,277,287 + bcr b2182 0.58 -0.5 2,277,287 + bcr b2182 0.58 +1.2 2,277,352 - bcr b2182 0.64 +0.0 2,277,352 - bcr b2182 0.64 +0.6 2,277,352 - bcr b2182 0.64 -0.3 2,277,422 - bcr b2182 0.70 +0.3 2,277,445 + bcr b2182 0.72 -0.1 2,277,573 + bcr b2182 0.82 +1.4 2,277,625 - bcr b2182 0.87 -0.9 2,277,628 - bcr b2182 0.87 -0.3 2,277,641 + bcr b2182 0.88 +0.5 2,277,695 + -2.2 2,277,695 + +0.0 2,277,696 + +1.7 2,277,759 + +0.7 2,277,767 + -0.3 2,278,002 - rsuA b2183 0.28 -0.3 2,278,018 - rsuA b2183 0.30 -1.9 2,278,126 - rsuA b2183 0.45 +0.7 2,278,173 + rsuA b2183 0.52 -0.3 2,278,266 - rsuA b2183 0.66 -0.1 2,278,285 - rsuA b2183 0.68 +0.1 2,278,462 - -0.5 2,278,560 - -0.5 2,278,678 - +0.4 2,278,720 + -0.3 2,278,720 + -0.3
Or see this region's nucleotide sequence