Strain Fitness in Escherichia coli BW25113 around b1672

Experiment: .5% DMSO

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydhU and ydhX overlap by 4 nucleotidesydhX and ydhW are separated by 63 nucleotidesydhW and ydhV are separated by 3 nucleotides b1670: ydhU - predicted cytochrome (NCBI), at 1,747,587 to 1,748,372 ydhU b1671: ydhX - predicted 4Fe-4S ferridoxin-type protein (RefSeq), at 1,748,369 to 1,749,037 ydhX b1672: ydhW - hypothetical protein (NCBI), at 1,749,101 to 1,749,748 ydhW b1673: ydhV - predicted oxidoreductase (NCBI), at 1,749,752 to 1,751,854 ydhV Position (kb) 1749 1750Strain fitness (log2 ratio) -1 0 1 2at 1748.231 kb on - strand, within ydhUat 1748.231 kb on - strand, within ydhUat 1748.764 kb on - strand, within ydhXat 1748.764 kb on - strand, within ydhXat 1748.847 kb on - strand, within ydhXat 1749.057 kb on - strandat 1749.057 kb on - strandat 1749.123 kb on + strandat 1749.144 kb on + strandat 1749.151 kb on - strandat 1749.425 kb on + strand, within ydhWat 1749.504 kb on - strand, within ydhWat 1749.504 kb on - strand, within ydhWat 1749.504 kb on - strand, within ydhWat 1749.607 kb on + strand, within ydhWat 1749.666 kb on - strand, within ydhWat 1749.802 kb on + strandat 1749.802 kb on + strandat 1750.415 kb on - strand, within ydhVat 1750.525 kb on + strand, within ydhVat 1750.525 kb on + strand, within ydhVat 1750.643 kb on - strand, within ydhVat 1750.662 kb on + strand, within ydhV

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Per-strain Table

Position Strand Gene LocusTag Fraction .5% DMSO
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1,748,231 - ydhU b1670 0.82 +0.5
1,748,231 - ydhU b1670 0.82 -1.0
1,748,764 - ydhX b1671 0.59 +0.5
1,748,764 - ydhX b1671 0.59 -0.2
1,748,847 - ydhX b1671 0.71 -0.5
1,749,057 - -1.3
1,749,057 - -0.5
1,749,123 + -0.3
1,749,144 + +0.5
1,749,151 - +1.0
1,749,425 + ydhW b1672 0.50 +1.8
1,749,504 - ydhW b1672 0.62 +0.3
1,749,504 - ydhW b1672 0.62 +0.2
1,749,504 - ydhW b1672 0.62 +0.2
1,749,607 + ydhW b1672 0.78 +1.3
1,749,666 - ydhW b1672 0.87 +0.2
1,749,802 + +0.8
1,749,802 + -0.1
1,750,415 - ydhV b1673 0.32 -0.1
1,750,525 + ydhV b1673 0.37 +0.5
1,750,525 + ydhV b1673 0.37 +0.5
1,750,643 - ydhV b1673 0.42 -0.9
1,750,662 + ydhV b1673 0.43 +0.5

Or see this region's nucleotide sequence