Strain Fitness in Escherichia coli BW25113 around b1025

Experiment: .5% DMSO

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycdS and ycdT are separated by 586 nucleotidesycdT and insF-4 are separated by 40 nucleotidesinsF-4 and insE overlap by 4 nucleotides b1024: ycdS - predicted outer membrane protein (NCBI), at 1,089,089 to 1,091,512 ycdS b1025: ycdT - predicted diguanylate cyclase (NCBI), at 1,092,099 to 1,093,457 ycdT b1026: insF-4 - IS3 element protein InsF (NCBI), at 1,093,498 to 1,094,364 insF-4 b1027: insE - IS3 element protein InsE (RefSeq), at 1,094,361 to 1,094,660 insE Position (kb) 1092 1093 1094Strain fitness (log2 ratio) -2 -1 0 1 2at 1091.116 kb on - strand, within ycdSat 1091.116 kb on - strand, within ycdSat 1091.128 kb on + strand, within ycdSat 1091.136 kb on - strand, within ycdSat 1091.136 kb on - strand, within ycdSat 1091.138 kb on - strand, within ycdSat 1091.138 kb on - strand, within ycdSat 1091.138 kb on - strand, within ycdSat 1091.138 kb on - strand, within ycdSat 1091.138 kb on - strand, within ycdSat 1091.153 kb on - strand, within ycdSat 1091.155 kb on + strand, within ycdSat 1091.155 kb on + strand, within ycdSat 1091.155 kb on + strand, within ycdSat 1091.155 kb on - strand, within ycdSat 1091.155 kb on - strand, within ycdSat 1091.161 kb on - strand, within ycdSat 1091.163 kb on - strand, within ycdSat 1091.163 kb on - strand, within ycdSat 1091.163 kb on - strand, within ycdSat 1091.163 kb on - strand, within ycdSat 1091.163 kb on - strand, within ycdSat 1091.163 kb on - strand, within ycdSat 1091.176 kb on + strand, within ycdSat 1091.176 kb on + strand, within ycdSat 1091.176 kb on + strand, within ycdSat 1091.177 kb on - strand, within ycdSat 1091.182 kb on + strand, within ycdSat 1091.182 kb on + strand, within ycdSat 1091.182 kb on + strand, within ycdSat 1091.182 kb on - strand, within ycdSat 1091.191 kb on - strand, within ycdSat 1091.195 kb on - strand, within ycdSat 1091.206 kb on + strand, within ycdSat 1091.206 kb on + strand, within ycdSat 1091.213 kb on + strand, within ycdSat 1091.213 kb on + strand, within ycdSat 1091.214 kb on - strand, within ycdSat 1091.223 kb on + strand, within ycdSat 1091.223 kb on + strand, within ycdSat 1091.233 kb on + strand, within ycdSat 1091.238 kb on + strand, within ycdSat 1091.238 kb on + strand, within ycdSat 1091.256 kb on + strand, within ycdSat 1091.263 kb on + strand, within ycdSat 1091.264 kb on - strand, within ycdSat 1091.283 kb on - strandat 1091.287 kb on + strandat 1091.290 kb on - strandat 1091.293 kb on + strandat 1091.301 kb on - strandat 1091.301 kb on - strandat 1091.301 kb on - strandat 1091.343 kb on + strandat 1091.353 kb on - strandat 1091.364 kb on + strandat 1091.364 kb on + strandat 1091.372 kb on - strandat 1091.405 kb on - strandat 1091.410 kb on + strandat 1091.413 kb on - strandat 1091.423 kb on - strandat 1091.425 kb on + strandat 1091.428 kb on - strandat 1091.432 kb on + strandat 1091.447 kb on - strandat 1091.511 kb on + strandat 1091.516 kb on + strandat 1091.519 kb on - strandat 1091.524 kb on - strandat 1091.528 kb on - strandat 1091.529 kb on - strandat 1091.529 kb on - strandat 1091.661 kb on + strandat 1091.661 kb on + strandat 1091.715 kb on - strandat 1091.716 kb on + strandat 1091.824 kb on + strandat 1091.832 kb on - strandat 1091.961 kb on - strandat 1091.998 kb on + strandat 1092.001 kb on + strandat 1092.004 kb on + strandat 1092.033 kb on + strandat 1092.071 kb on + strandat 1092.071 kb on + strandat 1092.074 kb on + strandat 1092.079 kb on + strandat 1092.079 kb on + strandat 1092.084 kb on + strandat 1092.086 kb on + strandat 1092.088 kb on + strandat 1092.089 kb on + strandat 1092.089 kb on + strandat 1092.089 kb on + strandat 1092.089 kb on + strandat 1092.089 kb on + strandat 1092.089 kb on + strandat 1092.097 kb on - strandat 1092.132 kb on + strandat 1092.141 kb on + strandat 1092.159 kb on + strandat 1092.222 kb on + strandat 1092.226 kb on + strandat 1093.014 kb on - strand, within ycdTat 1093.052 kb on - strand, within ycdTat 1093.087 kb on - strand, within ycdTat 1093.107 kb on - strand, within ycdTat 1093.168 kb on - strand, within ycdTat 1093.210 kb on + strand, within ycdTat 1093.210 kb on + strand, within ycdTat 1093.232 kb on + strand, within ycdTat 1093.312 kb on + strand, within ycdTat 1093.323 kb on + strandat 1093.323 kb on + strandat 1093.323 kb on + strandat 1093.331 kb on - strandat 1093.375 kb on + strandat 1093.375 kb on + strandat 1093.379 kb on - strandat 1093.416 kb on - strandat 1093.417 kb on - strandat 1093.417 kb on - strandat 1093.419 kb on + strandat 1093.419 kb on + strandat 1093.422 kb on + strandat 1093.426 kb on - strandat 1093.427 kb on - strandat 1093.493 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction .5% DMSO
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1,091,116 - ycdS b1024 0.84 -0.3
1,091,116 - ycdS b1024 0.84 -0.9
1,091,128 + ycdS b1024 0.84 -0.3
1,091,136 - ycdS b1024 0.84 -0.3
1,091,136 - ycdS b1024 0.84 -0.5
1,091,138 - ycdS b1024 0.85 +0.7
1,091,138 - ycdS b1024 0.85 -0.3
1,091,138 - ycdS b1024 0.85 -0.6
1,091,138 - ycdS b1024 0.85 +2.3
1,091,138 - ycdS b1024 0.85 -0.3
1,091,153 - ycdS b1024 0.85 +1.0
1,091,155 + ycdS b1024 0.85 -0.9
1,091,155 + ycdS b1024 0.85 -0.3
1,091,155 + ycdS b1024 0.85 +0.7
1,091,155 - ycdS b1024 0.85 +0.3
1,091,155 - ycdS b1024 0.85 -0.7
1,091,161 - ycdS b1024 0.85 +1.6
1,091,163 - ycdS b1024 0.86 +0.3
1,091,163 - ycdS b1024 0.86 +0.4
1,091,163 - ycdS b1024 0.86 -0.1
1,091,163 - ycdS b1024 0.86 -1.9
1,091,163 - ycdS b1024 0.86 -1.0
1,091,163 - ycdS b1024 0.86 -2.6
1,091,176 + ycdS b1024 0.86 -0.3
1,091,176 + ycdS b1024 0.86 -0.3
1,091,176 + ycdS b1024 0.86 -1.3
1,091,177 - ycdS b1024 0.86 -1.3
1,091,182 + ycdS b1024 0.86 -0.3
1,091,182 + ycdS b1024 0.86 -0.3
1,091,182 + ycdS b1024 0.86 +1.3
1,091,182 - ycdS b1024 0.86 +1.3
1,091,191 - ycdS b1024 0.87 -0.9
1,091,195 - ycdS b1024 0.87 +1.0
1,091,206 + ycdS b1024 0.87 -0.3
1,091,206 + ycdS b1024 0.87 -1.3
1,091,213 + ycdS b1024 0.88 -0.3
1,091,213 + ycdS b1024 0.88 +1.0
1,091,214 - ycdS b1024 0.88 -0.9
1,091,223 + ycdS b1024 0.88 -0.3
1,091,223 + ycdS b1024 0.88 -0.3
1,091,233 + ycdS b1024 0.88 +1.3
1,091,238 + ycdS b1024 0.89 -0.3
1,091,238 + ycdS b1024 0.89 +0.7
1,091,256 + ycdS b1024 0.89 +0.7
1,091,263 + ycdS b1024 0.90 -0.3
1,091,264 - ycdS b1024 0.90 -0.3
1,091,283 - +1.3
1,091,287 + -0.3
1,091,290 - -0.3
1,091,293 + +1.3
1,091,301 - -1.3
1,091,301 - +0.7
1,091,301 - -0.3
1,091,343 + +0.1
1,091,353 - +0.7
1,091,364 + -1.3
1,091,364 + -1.0
1,091,372 - -0.3
1,091,405 - -0.3
1,091,410 + -0.3
1,091,413 - -0.3
1,091,423 - -0.3
1,091,425 + +0.7
1,091,428 - -0.3
1,091,432 + -1.3
1,091,447 - -1.5
1,091,511 + -0.3
1,091,516 + -0.3
1,091,519 - +0.3
1,091,524 - -1.3
1,091,528 - +0.3
1,091,529 - -0.3
1,091,529 - +1.3
1,091,661 + -0.3
1,091,661 + -0.9
1,091,715 - -0.3
1,091,716 + -0.3
1,091,824 + -0.3
1,091,832 - -0.3
1,091,961 - -0.3
1,091,998 + -0.3
1,092,001 + -1.3
1,092,004 + -1.9
1,092,033 + +0.7
1,092,071 + +0.8
1,092,071 + -0.5
1,092,074 + +0.9
1,092,079 + -1.3
1,092,079 + -0.3
1,092,084 + -0.6
1,092,086 + -0.3
1,092,088 + +0.4
1,092,089 + -1.3
1,092,089 + -1.3
1,092,089 + +1.1
1,092,089 + +0.3
1,092,089 + -0.3
1,092,089 + -0.3
1,092,097 - -0.3
1,092,132 + -0.3
1,092,141 + -0.3
1,092,159 + -0.3
1,092,222 + -0.3
1,092,226 + -0.3
1,093,014 - ycdT b1025 0.67 +0.7
1,093,052 - ycdT b1025 0.70 -0.3
1,093,087 - ycdT b1025 0.73 +1.0
1,093,107 - ycdT b1025 0.74 -0.3
1,093,168 - ycdT b1025 0.79 -0.3
1,093,210 + ycdT b1025 0.82 +1.3
1,093,210 + ycdT b1025 0.82 -0.3
1,093,232 + ycdT b1025 0.83 +0.3
1,093,312 + ycdT b1025 0.89 +1.7
1,093,323 + -0.3
1,093,323 + -0.3
1,093,323 + -0.9
1,093,331 - -0.3
1,093,375 + -0.3
1,093,375 + -1.3
1,093,379 - -1.3
1,093,416 - -0.3
1,093,417 - -0.3
1,093,417 - -0.3
1,093,419 + -1.3
1,093,419 + +2.3
1,093,422 + -0.3
1,093,426 - -2.6
1,093,427 - -0.3
1,093,493 - -0.3

Or see this region's nucleotide sequence