Strain Fitness in Escherichia coli BW25113 around b0503

Experiment: .5% DMSO

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntylbI and ylbG are separated by 2 nucleotidesylbG and ybbB are separated by 115 nucleotidesybbB and ybbS are separated by 68 nucleotides b4632: ylbI - no description, at 528,724 to 528,816 ylbI b0502: ylbG - no description, at 528,819 to 529,240 ylbG b0503: ybbB - tRNA 2-selenouridine synthase, selenophosphate-dependent (NCBI), at 529,356 to 530,450 ybbB b0504: ybbS - DNA-binding transcriptional activator of the allD operon (NCBI), at 530,519 to 531,445 ybbS Position (kb) 529 530 531Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 528.377 kb on - strandat 528.384 kb on + strandat 528.446 kb on + strandat 528.454 kb on + strandat 528.502 kb on + strandat 528.502 kb on + strandat 528.512 kb on + strandat 528.750 kb on - strand, within ylbIat 528.757 kb on + strand, within ylbIat 528.757 kb on + strand, within ylbIat 528.759 kb on + strand, within ylbIat 528.765 kb on - strand, within ylbIat 528.765 kb on - strand, within ylbIat 528.765 kb on - strand, within ylbIat 528.765 kb on - strand, within ylbIat 528.765 kb on - strand, within ylbIat 528.767 kb on - strand, within ylbIat 528.770 kb on - strand, within ylbIat 528.770 kb on - strand, within ylbIat 528.770 kb on - strand, within ylbIat 528.777 kb on + strand, within ylbIat 528.777 kb on + strand, within ylbIat 528.786 kb on - strand, within ylbIat 528.786 kb on - strand, within ylbIat 528.786 kb on - strand, within ylbIat 528.786 kb on - strand, within ylbIat 528.793 kb on - strand, within ylbIat 528.799 kb on - strand, within ylbIat 528.803 kb on - strand, within ylbIat 528.803 kb on - strand, within ylbIat 528.834 kb on - strandat 528.834 kb on - strandat 528.880 kb on + strand, within ylbGat 528.897 kb on - strand, within ylbGat 528.897 kb on - strand, within ylbGat 528.969 kb on + strand, within ylbGat 529.243 kb on + strandat 529.243 kb on + strandat 529.243 kb on + strandat 529.249 kb on - strandat 529.269 kb on + strandat 529.312 kb on - strandat 529.437 kb on - strandat 529.728 kb on - strand, within ybbBat 529.728 kb on - strand, within ybbBat 529.906 kb on - strand, within ybbBat 529.911 kb on + strand, within ybbBat 529.911 kb on + strand, within ybbBat 529.959 kb on + strand, within ybbBat 529.959 kb on + strand, within ybbBat 530.005 kb on - strand, within ybbBat 530.209 kb on + strand, within ybbBat 530.583 kb on - strandat 530.583 kb on - strandat 531.058 kb on - strand, within ybbSat 531.077 kb on - strand, within ybbSat 531.113 kb on + strand, within ybbSat 531.134 kb on - strand, within ybbSat 531.229 kb on + strand, within ybbS

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Per-strain Table

Position Strand Gene LocusTag Fraction .5% DMSO
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528,377 - -0.1
528,384 + -0.1
528,446 + -0.1
528,454 + -0.1
528,502 + -0.1
528,502 + -0.1
528,512 + -0.1
528,750 - ylbI b4632 0.28 -0.1
528,757 + ylbI b4632 0.35 -0.1
528,757 + ylbI b4632 0.35 -0.1
528,759 + ylbI b4632 0.38 -0.1
528,765 - ylbI b4632 0.44 +0.5
528,765 - ylbI b4632 0.44 -0.6
528,765 - ylbI b4632 0.44 +0.0
528,765 - ylbI b4632 0.44 +0.6
528,765 - ylbI b4632 0.44 +0.6
528,767 - ylbI b4632 0.46 -1.4
528,770 - ylbI b4632 0.49 -0.3
528,770 - ylbI b4632 0.49 -0.6
528,770 - ylbI b4632 0.49 +0.9
528,777 + ylbI b4632 0.57 +0.7
528,777 + ylbI b4632 0.57 -0.3
528,786 - ylbI b4632 0.67 +2.8
528,786 - ylbI b4632 0.67 -0.1
528,786 - ylbI b4632 0.67 -0.1
528,786 - ylbI b4632 0.67 +0.3
528,793 - ylbI b4632 0.74 +0.3
528,799 - ylbI b4632 0.81 -0.7
528,803 - ylbI b4632 0.85 -1.6
528,803 - ylbI b4632 0.85 -0.3
528,834 - +0.5
528,834 - +0.9
528,880 + ylbG b0502 0.14 -0.4
528,897 - ylbG b0502 0.18 +1.5
528,897 - ylbG b0502 0.18 +1.2
528,969 + ylbG b0502 0.36 +1.4
529,243 + -0.6
529,243 + -0.1
529,243 + -1.1
529,249 - -0.1
529,269 + -0.1
529,312 - +0.4
529,437 - -0.7
529,728 - ybbB b0503 0.34 -0.8
529,728 - ybbB b0503 0.34 -0.8
529,906 - ybbB b0503 0.50 +0.4
529,911 + ybbB b0503 0.51 -1.1
529,911 + ybbB b0503 0.51 +0.9
529,959 + ybbB b0503 0.55 -1.1
529,959 + ybbB b0503 0.55 -0.6
530,005 - ybbB b0503 0.59 +3.9
530,209 + ybbB b0503 0.78 +0.9
530,583 - +0.9
530,583 - -0.3
531,058 - ybbS b0504 0.58 -1.8
531,077 - ybbS b0504 0.60 +0.4
531,113 + ybbS b0504 0.64 -0.3
531,134 - ybbS b0504 0.66 -0.9
531,229 + ybbS b0504 0.77 +1.4

Or see this region's nucleotide sequence