Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fusA and rpsG are separated by 27 nucleotides rpsG and rpsL are separated by 96 nucleotides rpsL and yheL are separated by 125 nucleotides yheL and yheM are separated by 7 nucleotides
b3340: fusA - elongation factor EF-2 (NCBI), at 3,469,422 to 3,471,536
fusA
b3341: rpsG - 30S ribosomal protein S7 (NCBI), at 3,471,564 to 3,472,103
rpsG
b3342: rpsL - 30S ribosomal protein S12 (NCBI), at 3,472,200 to 3,472,574
rpsL
b3343: yheL - predicted intracellular sulfur oxidation protein (NCBI), at 3,472,700 to 3,472,987
yheL
b3344: yheM - hypothetical protein (NCBI), at 3,472,995 to 3,473,354
yheM
Position (kb)
3471
3472
3473 Strain fitness (log2 ratio)
-2
-1
0
1 at 3471.616 kb on - strand at 3471.643 kb on - strand, within rpsG at 3471.643 kb on - strand, within rpsG at 3471.645 kb on - strand at 3471.645 kb on - strand, within rpsG at 3472.212 kb on - strand at 3472.640 kb on - strand at 3472.640 kb on - strand at 3472.681 kb on + strand at 3472.681 kb on - strand at 3472.987 kb on - strand at 3472.987 kb on - strand at 3472.999 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,471,616 - -1.7 3,471,643 - rpsG b3341 0.15 -0.8 3,471,643 - rpsG b3341 0.15 -0.8 3,471,645 - +0.2 3,471,645 - rpsG b3341 0.15 +1.2 3,472,212 - +0.2 3,472,640 - +0.2 3,472,640 - -1.1 3,472,681 + -2.6 3,472,681 - -1.3 3,472,987 - -0.8 3,472,987 - +0.2 3,472,999 - +0.7
Or see this region's nucleotide sequence