Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygdE and ygdD overlap by 8 nucleotides ygdD and gcvA are separated by 18 nucleotides gcvA and gcvB are separated by 128 nucleotides gcvB and b2809 are separated by 16 nucleotides b2809 and csdA are separated by 191 nucleotides
b2806: ygdE - predicted methyltransferase (NCBI), at 2,938,165 to 2,939,265
ygdE
b2807: ygdD - conserved inner membrane protein (NCBI), at 2,939,258 to 2,939,653
ygdD
b2808: gcvA - DNA-binding transcriptional dual regulator (NCBI), at 2,939,672 to 2,940,589
gcvA
b4443: gcvB - GcvB sRNA gene divergent from gcvA (RefSeq), at 2,940,718 to 2,940,923
gcvB
b2809: b2809 - orf, hypothetical protein (VIMSS), at 2,940,940 to 2,941,167
b2809
b2810: csdA - cysteine sulfinate desulfinase (NCBI), at 2,941,359 to 2,942,564
csdA
Position (kb)
2939
2940
2941 Strain fitness (log2 ratio)
-2
-1
0
1 at 2938.698 kb on + strand, within ygdE at 2938.711 kb on - strand, within ygdE at 2938.711 kb on - strand, within ygdE at 2938.750 kb on - strand, within ygdE at 2938.758 kb on + strand, within ygdE at 2938.780 kb on - strand, within ygdE at 2938.780 kb on - strand, within ygdE at 2938.795 kb on - strand, within ygdE at 2938.795 kb on - strand, within ygdE at 2938.810 kb on + strand, within ygdE at 2938.810 kb on + strand, within ygdE at 2938.868 kb on - strand, within ygdE at 2938.883 kb on - strand, within ygdE at 2938.969 kb on + strand, within ygdE at 2938.974 kb on + strand, within ygdE at 2938.974 kb on + strand, within ygdE at 2938.986 kb on + strand, within ygdE at 2939.038 kb on - strand, within ygdE at 2939.298 kb on - strand, within ygdD at 2939.347 kb on + strand, within ygdD at 2939.405 kb on + strand, within ygdD at 2939.442 kb on - strand, within ygdD at 2939.465 kb on - strand, within ygdD at 2939.470 kb on + strand, within ygdD at 2939.470 kb on + strand, within ygdD at 2939.545 kb on + strand, within ygdD at 2939.553 kb on - strand, within ygdD at 2939.648 kb on + strand at 2939.648 kb on + strand at 2939.655 kb on + strand at 2939.663 kb on - strand at 2939.663 kb on - strand at 2939.778 kb on - strand, within gcvA at 2939.778 kb on - strand, within gcvA at 2939.804 kb on + strand, within gcvA at 2939.912 kb on - strand, within gcvA at 2939.914 kb on - strand, within gcvA at 2939.982 kb on - strand, within gcvA at 2939.982 kb on - strand, within gcvA at 2939.985 kb on - strand, within gcvA at 2939.987 kb on - strand, within gcvA at 2940.001 kb on + strand, within gcvA at 2940.068 kb on + strand, within gcvA at 2940.171 kb on - strand, within gcvA at 2940.171 kb on - strand, within gcvA at 2940.289 kb on + strand, within gcvA at 2940.331 kb on + strand, within gcvA at 2940.399 kb on - strand, within gcvA at 2940.417 kb on - strand, within gcvA at 2940.477 kb on - strand, within gcvA at 2940.522 kb on + strand at 2940.587 kb on - strand at 2940.719 kb on + strand at 2940.838 kb on - strand, within gcvB at 2940.909 kb on - strand at 2941.082 kb on - strand, within b2809 at 2941.082 kb on - strand, within b2809 at 2941.096 kb on - strand, within b2809 at 2941.096 kb on - strand, within b2809 at 2941.157 kb on + strand at 2941.157 kb on + strand at 2941.203 kb on + strand at 2941.203 kb on + strand at 2941.239 kb on + strand at 2941.254 kb on - strand at 2941.254 kb on - strand at 2941.422 kb on + strand at 2941.422 kb on + strand at 2941.425 kb on + strand at 2941.430 kb on - strand at 2941.430 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,938,698 + ygdE b2806 0.48 -0.5 2,938,711 - ygdE b2806 0.50 +1.1 2,938,711 - ygdE b2806 0.50 +0.0 2,938,750 - ygdE b2806 0.53 -0.2 2,938,758 + ygdE b2806 0.54 -0.3 2,938,780 - ygdE b2806 0.56 +1.0 2,938,780 - ygdE b2806 0.56 -0.2 2,938,795 - ygdE b2806 0.57 +0.4 2,938,795 - ygdE b2806 0.57 -0.7 2,938,810 + ygdE b2806 0.59 -1.6 2,938,810 + ygdE b2806 0.59 +0.8 2,938,868 - ygdE b2806 0.64 -0.6 2,938,883 - ygdE b2806 0.65 +0.3 2,938,969 + ygdE b2806 0.73 +0.6 2,938,974 + ygdE b2806 0.73 -1.3 2,938,974 + ygdE b2806 0.73 -0.5 2,938,986 + ygdE b2806 0.75 +0.3 2,939,038 - ygdE b2806 0.79 +0.0 2,939,298 - ygdD b2807 0.10 -0.2 2,939,347 + ygdD b2807 0.22 -2.0 2,939,405 + ygdD b2807 0.37 -0.3 2,939,442 - ygdD b2807 0.46 +0.3 2,939,465 - ygdD b2807 0.52 -1.0 2,939,470 + ygdD b2807 0.54 -0.2 2,939,470 + ygdD b2807 0.54 -1.3 2,939,545 + ygdD b2807 0.72 +0.0 2,939,553 - ygdD b2807 0.74 -1.0 2,939,648 + -0.4 2,939,648 + -0.6 2,939,655 + -1.2 2,939,663 - -0.3 2,939,663 - +1.0 2,939,778 - gcvA b2808 0.12 -1.6 2,939,778 - gcvA b2808 0.12 -0.3 2,939,804 + gcvA b2808 0.14 -1.6 2,939,912 - gcvA b2808 0.26 -1.6 2,939,914 - gcvA b2808 0.26 -1.0 2,939,982 - gcvA b2808 0.34 -1.6 2,939,982 - gcvA b2808 0.34 -1.3 2,939,985 - gcvA b2808 0.34 -1.6 2,939,987 - gcvA b2808 0.34 -1.0 2,940,001 + gcvA b2808 0.36 +1.0 2,940,068 + gcvA b2808 0.43 +0.0 2,940,171 - gcvA b2808 0.54 +0.0 2,940,171 - gcvA b2808 0.54 +0.0 2,940,289 + gcvA b2808 0.67 -0.7 2,940,331 + gcvA b2808 0.72 -1.0 2,940,399 - gcvA b2808 0.79 -1.0 2,940,417 - gcvA b2808 0.81 -1.0 2,940,477 - gcvA b2808 0.88 -1.5 2,940,522 + +0.0 2,940,587 - -0.3 2,940,719 + -1.4 2,940,838 - gcvB b4443 0.58 +0.0 2,940,909 - +0.0 2,941,082 - b2809 b2809 0.62 -0.5 2,941,082 - b2809 b2809 0.62 +0.0 2,941,096 - b2809 b2809 0.68 +0.0 2,941,096 - b2809 b2809 0.68 +1.0 2,941,157 + +0.8 2,941,157 + -1.0 2,941,203 + -1.3 2,941,203 + -0.5 2,941,239 + -0.9 2,941,254 - +1.0 2,941,254 - +1.6 2,941,422 + -1.0 2,941,422 + +1.0 2,941,425 + -0.7 2,941,430 - -1.2 2,941,430 - -0.7
Or see this region's nucleotide sequence