Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dcp and ydfG are separated by 136 nucleotides ydfG and ydfH are separated by 88 nucleotides ydfH and ydfZ are separated by 176 nucleotides ydfZ and ydfI are separated by 34 nucleotides
b1538: dcp - dipeptidyl carboxypeptidase II (NCBI), at 1,623,359 to 1,625,404
dcp
b1539: ydfG - L-allo-threonine dehydrogenase, NAD(P)-binding (NCBI), at 1,625,541 to 1,626,287
ydfG
b1540: ydfH - predicted DNA-binding transcriptional regulator (NCBI), at 1,626,376 to 1,627,062
ydfH
b1541: ydfZ - hypothetical protein (NCBI), at 1,627,239 to 1,627,442
ydfZ
b1542: ydfI - predicted mannonate dehydrogenase (NCBI), at 1,627,477 to 1,628,937
ydfI
Position (kb)
1626
1627
1628 Strain fitness (log2 ratio)
-2
-1
0
1 at 1625.380 kb on + strand at 1625.442 kb on - strand at 1625.443 kb on - strand at 1625.443 kb on - strand at 1625.681 kb on + strand, within ydfG at 1625.871 kb on + strand, within ydfG at 1625.871 kb on + strand, within ydfG at 1626.043 kb on - strand, within ydfG at 1626.461 kb on + strand, within ydfH at 1626.955 kb on + strand, within ydfH at 1627.253 kb on - strand at 1627.424 kb on - strand at 1627.446 kb on + strand at 1627.454 kb on - strand at 1627.556 kb on - strand at 1627.675 kb on - strand, within ydfI at 1627.873 kb on + strand, within ydfI
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,625,380 + +0.3 1,625,442 - -0.4 1,625,443 - -0.8 1,625,443 - -0.0 1,625,681 + ydfG b1539 0.19 +0.5 1,625,871 + ydfG b1539 0.44 +0.2 1,625,871 + ydfG b1539 0.44 +0.4 1,626,043 - ydfG b1539 0.67 -0.1 1,626,461 + ydfH b1540 0.12 -1.9 1,626,955 + ydfH b1540 0.84 -0.9 1,627,253 - +0.1 1,627,424 - +0.4 1,627,446 + +0.4 1,627,454 - -1.8 1,627,556 - -0.6 1,627,675 - ydfI b1542 0.14 +1.5 1,627,873 + ydfI b1542 0.27 +1.0
Or see this region's nucleotide sequence