Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b1355 and racR are separated by 308 nucleotides racR and ydaS are separated by 123 nucleotides ydaS and ydaT are separated by 22 nucleotides ydaT and ydaU are separated by 12 nucleotides
b1355: b1355 - orf, hypothetical protein (VIMSS), at 1,417,346 to 1,417,480
b1355
b1356: racR - Rac prophage; predicted DNA-binding transcriptional regulator (NCBI), at 1,417,789 to 1,418,265
racR
b1357: ydaS - Rac prophage; predicted DNA-binding transcriptional regulator (NCBI), at 1,418,389 to 1,418,685
ydaS
b1358: ydaT - Rac prophage; predicted protein (NCBI), at 1,418,708 to 1,419,130
ydaT
b1359: ydaU - Rac prophage; conserved protein (NCBI), at 1,419,143 to 1,420,000
ydaU
Position (kb)
1418
1419 Strain fitness (log2 ratio)
-1
0
1
2 at 1417.427 kb on + strand, within b1355 at 1417.431 kb on - strand, within b1355 at 1417.459 kb on + strand, within b1355 at 1417.518 kb on - strand at 1417.574 kb on + strand at 1418.573 kb on - strand, within ydaS at 1418.619 kb on - strand, within ydaS at 1418.746 kb on - strand at 1418.848 kb on - strand, within ydaT at 1418.848 kb on - strand, within ydaT at 1419.186 kb on + strand at 1419.186 kb on + strand at 1419.235 kb on - strand, within ydaU at 1419.360 kb on - strand, within ydaU at 1419.393 kb on - strand, within ydaU at 1419.393 kb on - strand, within ydaU
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,417,427 + b1355 b1355 0.60 +0.3 1,417,431 - b1355 b1355 0.63 -0.5 1,417,459 + b1355 b1355 0.84 -0.8 1,417,518 - -0.5 1,417,574 + +0.7 1,418,573 - ydaS b1357 0.62 -1.1 1,418,619 - ydaS b1357 0.77 -0.5 1,418,746 - +1.6 1,418,848 - ydaT b1358 0.33 -0.5 1,418,848 - ydaT b1358 0.33 -0.5 1,419,186 + +1.8 1,419,186 + +0.7 1,419,235 - ydaU b1359 0.11 +0.0 1,419,360 - ydaU b1359 0.25 -0.3 1,419,393 - ydaU b1359 0.29 -0.5 1,419,393 - ydaU b1359 0.29 -0.5
Or see this region's nucleotide sequence