Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sapF and sapD are separated by 1 nucleotides sapD and sapC overlap by 1 nucleotides sapC and sapB overlap by 14 nucleotides sapB and sapA overlap by 4 nucleotides
b1290: sapF - predicted antimicrobial peptide transporter subunit (NCBI), at 1,349,852 to 1,350,658
sapF
b1291: sapD - predicted antimicrobial peptide transporter subunit (NCBI), at 1,350,660 to 1,351,652
sapD
b1292: sapC - predicted antimicrobial peptide transporter subunit (NCBI), at 1,351,652 to 1,352,542
sapC
b1293: sapB - predicted antimicrobial peptide transporter subunit (NCBI), at 1,352,529 to 1,353,494
sapB
b1294: sapA - predicted antimicrobial peptide transporter subunit (NCBI), at 1,353,491 to 1,355,134
sapA
Position (kb)
1351
1352
1353 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1350.656 kb on - strand at 1350.749 kb on + strand at 1350.855 kb on + strand, within sapD at 1350.861 kb on + strand, within sapD at 1350.997 kb on - strand, within sapD at 1351.021 kb on - strand, within sapD at 1351.342 kb on - strand, within sapD at 1351.739 kb on - strand at 1352.074 kb on - strand, within sapC at 1352.113 kb on + strand, within sapC at 1352.173 kb on + strand, within sapC at 1352.173 kb on + strand, within sapC at 1352.254 kb on + strand, within sapC at 1352.290 kb on - strand, within sapC at 1352.351 kb on - strand, within sapC at 1352.438 kb on + strand, within sapC at 1352.770 kb on - strand, within sapB at 1352.770 kb on - strand, within sapB at 1352.790 kb on + strand, within sapB at 1353.131 kb on + strand, within sapB at 1353.522 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,350,656 - -0.4 1,350,749 + -1.4 1,350,855 + sapD b1291 0.20 +0.6 1,350,861 + sapD b1291 0.20 -0.4 1,350,997 - sapD b1291 0.34 +0.6 1,351,021 - sapD b1291 0.36 -0.4 1,351,342 - sapD b1291 0.69 -0.4 1,351,739 - -2.0 1,352,074 - sapC b1292 0.47 -0.4 1,352,113 + sapC b1292 0.52 -2.8 1,352,173 + sapC b1292 0.58 +0.6 1,352,173 + sapC b1292 0.58 -0.4 1,352,254 + sapC b1292 0.68 -2.0 1,352,290 - sapC b1292 0.72 -1.4 1,352,351 - sapC b1292 0.78 +0.1 1,352,438 + sapC b1292 0.88 -1.4 1,352,770 - sapB b1293 0.25 -1.0 1,352,770 - sapB b1293 0.25 -1.0 1,352,790 + sapB b1293 0.27 -1.5 1,353,131 + sapB b1293 0.62 +0.5 1,353,522 + -1.5
Or see this region's nucleotide sequence