Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafD and yafE are separated by 3 nucleotides yafE and mltD are separated by 47 nucleotides mltD and gloB are separated by 71 nucleotides gloB and yafS are separated by 33 nucleotides
b0209: yafD - hypothetical protein (NCBI), at 231,122 to 231,922
yafD
b0210: yafE - predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 231,926 to 232,549
yafE
b0211: mltD - predicted membrane-bound lytic murein transglycosylase D (NCBI), at 232,597 to 233,955
mltD
b0212: gloB - predicted hydroxyacylglutathione hydrolase (NCBI), at 234,027 to 234,782
gloB
b0213: yafS - orf, hypothetical protein (VIMSS), at 234,816 to 235,538
yafS
Position (kb) 232
233
234 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 231.601 kb on + strand at 231.621 kb on + strand, within yafD at 231.627 kb on - strand, within yafD at 231.627 kb on - strand, within yafD at 231.659 kb on + strand, within yafD at 231.659 kb on + strand, within yafD at 231.664 kb on - strand, within yafD at 231.664 kb on - strand, within yafD at 231.667 kb on - strand, within yafD at 231.755 kb on + strand, within yafD at 231.828 kb on + strand, within yafD at 231.828 kb on + strand, within yafD at 231.837 kb on + strand, within yafD at 231.851 kb on + strand at 231.851 kb on + strand at 231.935 kb on - strand at 231.935 kb on - strand at 231.938 kb on + strand at 231.946 kb on - strand at 231.970 kb on + strand at 231.983 kb on + strand at 231.983 kb on + strand at 232.010 kb on + strand, within yafE at 232.254 kb on + strand, within yafE at 232.254 kb on + strand, within yafE at 232.254 kb on + strand, within yafE at 232.303 kb on + strand, within yafE at 232.469 kb on + strand, within yafE at 232.656 kb on + strand at 232.678 kb on - strand at 232.728 kb on - strand at 232.728 kb on - strand at 232.760 kb on - strand, within mltD at 232.830 kb on + strand, within mltD at 232.830 kb on + strand, within mltD at 232.839 kb on + strand, within mltD at 232.839 kb on + strand, within mltD at 232.923 kb on - strand, within mltD at 232.923 kb on - strand, within mltD at 233.026 kb on - strand, within mltD at 233.047 kb on - strand, within mltD at 233.065 kb on - strand, within mltD at 233.075 kb on - strand, within mltD at 233.075 kb on - strand, within mltD at 233.156 kb on + strand, within mltD at 233.376 kb on - strand, within mltD at 233.376 kb on - strand, within mltD at 233.472 kb on + strand, within mltD at 233.472 kb on + strand, within mltD at 233.498 kb on - strand, within mltD at 233.697 kb on - strand, within mltD at 233.698 kb on - strand, within mltD at 233.760 kb on - strand, within mltD at 233.760 kb on - strand, within mltD at 233.774 kb on + strand, within mltD at 233.774 kb on + strand, within mltD at 233.875 kb on + strand at 233.914 kb on + strand at 234.054 kb on - strand at 234.253 kb on - strand, within gloB at 234.292 kb on + strand, within gloB at 234.292 kb on + strand, within gloB at 234.386 kb on + strand, within gloB at 234.386 kb on - strand, within gloB at 234.556 kb on - strand, within gloB at 234.737 kb on - strand at 234.737 kb on - strand at 234.796 kb on + strand at 234.796 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 231,601 + +0.0 231,621 + yafD b0209 0.62 -0.7 231,627 - yafD b0209 0.63 -0.2 231,627 - yafD b0209 0.63 +0.5 231,659 + yafD b0209 0.67 -1.8 231,659 + yafD b0209 0.67 +0.3 231,664 - yafD b0209 0.68 +0.0 231,664 - yafD b0209 0.68 +1.2 231,667 - yafD b0209 0.68 -1.4 231,755 + yafD b0209 0.79 -0.4 231,828 + yafD b0209 0.88 +0.0 231,828 + yafD b0209 0.88 +1.0 231,837 + yafD b0209 0.89 -0.9 231,851 + +0.4 231,851 + +0.4 231,935 - +0.3 231,935 - +0.3 231,938 + +1.7 231,946 - -0.2 231,970 + +0.6 231,983 + -1.4 231,983 + +1.0 232,010 + yafE b0210 0.13 -0.9 232,254 + yafE b0210 0.53 +0.6 232,254 + yafE b0210 0.53 +1.3 232,254 + yafE b0210 0.53 -1.0 232,303 + yafE b0210 0.60 +2.8 232,469 + yafE b0210 0.87 +0.4 232,656 + +0.1 232,678 - -0.1 232,728 - -0.4 232,728 - -0.5 232,760 - mltD b0211 0.12 -0.6 232,830 + mltD b0211 0.17 -1.2 232,830 + mltD b0211 0.17 -0.1 232,839 + mltD b0211 0.18 +0.2 232,839 + mltD b0211 0.18 -1.2 232,923 - mltD b0211 0.24 +0.6 232,923 - mltD b0211 0.24 +1.0 233,026 - mltD b0211 0.32 -0.6 233,047 - mltD b0211 0.33 +0.8 233,065 - mltD b0211 0.34 -0.2 233,075 - mltD b0211 0.35 -0.4 233,075 - mltD b0211 0.35 -3.6 233,156 + mltD b0211 0.41 -0.6 233,376 - mltD b0211 0.57 +0.8 233,376 - mltD b0211 0.57 +0.6 233,472 + mltD b0211 0.64 +0.2 233,472 + mltD b0211 0.64 +0.6 233,498 - mltD b0211 0.66 +0.2 233,697 - mltD b0211 0.81 +0.5 233,698 - mltD b0211 0.81 -0.1 233,760 - mltD b0211 0.86 +0.6 233,760 - mltD b0211 0.86 +0.4 233,774 + mltD b0211 0.87 +0.0 233,774 + mltD b0211 0.87 +2.0 233,875 + -1.0 233,914 + -1.2 234,054 - +0.3 234,253 - gloB b0212 0.30 +0.0 234,292 + gloB b0212 0.35 +0.0 234,292 + gloB b0212 0.35 +1.3 234,386 + gloB b0212 0.47 -1.6 234,386 - gloB b0212 0.47 -0.3 234,556 - gloB b0212 0.70 -1.4 234,737 - +1.0 234,737 - +2.3 234,796 + -0.4 234,796 + +2.3
Or see this region's nucleotide sequence