Experiment: Methyl beta-D-glucopyranoside (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu6127 and Atu6128 overlap by 4 nucleotides Atu6128 and Atu6129 are separated by 88 nucleotides
Atu6127: Atu6127 - OriT nicking enzyme, Dtr system, at 143,987 to 147,289
Atu6127
Atu6128: Atu6128 - Conjugative pilin processing protease, at 147,286 to 147,816
Atu6128
Atu6129: Atu6129 - conjugal transfer protein, at 147,905 to 149,002
Atu6129
Position (kb)
147
148 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 146.376 kb on + strand, within Atu6127 at 146.382 kb on - strand, within Atu6127 at 146.421 kb on + strand, within Atu6127 at 146.429 kb on - strand, within Atu6127 at 146.456 kb on - strand, within Atu6127 at 146.531 kb on - strand, within Atu6127 at 146.677 kb on - strand, within Atu6127 at 146.677 kb on - strand, within Atu6127 at 146.716 kb on + strand, within Atu6127 at 146.755 kb on + strand, within Atu6127 at 146.794 kb on + strand, within Atu6127 at 146.794 kb on + strand, within Atu6127 at 146.794 kb on + strand, within Atu6127 at 146.794 kb on + strand, within Atu6127 at 146.941 kb on + strand, within Atu6127 at 146.941 kb on + strand, within Atu6127 at 146.947 kb on - strand, within Atu6127 at 147.050 kb on - strand at 147.077 kb on + strand at 147.077 kb on + strand at 147.082 kb on + strand at 147.141 kb on + strand at 147.164 kb on + strand at 147.212 kb on - strand at 147.236 kb on - strand at 147.288 kb on + strand at 147.301 kb on + strand at 147.301 kb on + strand at 147.331 kb on + strand at 147.339 kb on - strand, within Atu6128 at 147.339 kb on - strand, within Atu6128 at 147.469 kb on + strand, within Atu6128 at 147.515 kb on + strand, within Atu6128 at 147.525 kb on + strand, within Atu6128 at 147.535 kb on - strand, within Atu6128 at 147.535 kb on - strand, within Atu6128 at 147.536 kb on + strand, within Atu6128 at 147.627 kb on + strand, within Atu6128 at 147.651 kb on + strand, within Atu6128 at 147.673 kb on + strand, within Atu6128 at 147.749 kb on + strand, within Atu6128 at 147.757 kb on - strand, within Atu6128 at 147.768 kb on - strand at 147.768 kb on - strand at 147.783 kb on - strand at 147.796 kb on + strand at 147.803 kb on + strand at 147.804 kb on - strand at 147.806 kb on - strand at 147.806 kb on - strand at 147.835 kb on + strand at 147.902 kb on + strand at 148.005 kb on - strand at 148.023 kb on - strand, within Atu6129 at 148.049 kb on - strand, within Atu6129 at 148.101 kb on - strand, within Atu6129 at 148.101 kb on - strand, within Atu6129 at 148.141 kb on + strand, within Atu6129 at 148.141 kb on + strand, within Atu6129 at 148.149 kb on - strand, within Atu6129 at 148.198 kb on - strand, within Atu6129 at 148.223 kb on + strand, within Atu6129 at 148.274 kb on + strand, within Atu6129 at 148.372 kb on + strand, within Atu6129 at 148.380 kb on - strand, within Atu6129 at 148.403 kb on + strand, within Atu6129 at 148.411 kb on - strand, within Atu6129 at 148.420 kb on + strand, within Atu6129 at 148.420 kb on + strand, within Atu6129 at 148.428 kb on - strand, within Atu6129 at 148.458 kb on + strand, within Atu6129 at 148.470 kb on + strand, within Atu6129 at 148.650 kb on - strand, within Atu6129 at 148.655 kb on + strand, within Atu6129 at 148.655 kb on + strand, within Atu6129 at 148.663 kb on - strand, within Atu6129 at 148.678 kb on + strand, within Atu6129 at 148.752 kb on - strand, within Atu6129 at 148.787 kb on + strand, within Atu6129
Per-strain Table
Position Strand Gene LocusTag Fraction Methyl beta-D-glucopyranoside (C) remove 146,376 + Atu6127 0.72 -0.7 146,382 - Atu6127 0.73 +0.2 146,421 + Atu6127 0.74 +0.0 146,429 - Atu6127 0.74 -0.7 146,456 - Atu6127 0.75 +0.4 146,531 - Atu6127 0.77 -0.8 146,677 - Atu6127 0.81 -0.7 146,677 - Atu6127 0.81 -0.0 146,716 + Atu6127 0.83 +0.4 146,755 + Atu6127 0.84 +0.6 146,794 + Atu6127 0.85 +0.5 146,794 + Atu6127 0.85 +0.8 146,794 + Atu6127 0.85 +0.8 146,794 + Atu6127 0.85 +0.1 146,941 + Atu6127 0.89 +1.9 146,941 + Atu6127 0.89 +0.3 146,947 - Atu6127 0.90 -0.2 147,050 - -0.4 147,077 + -0.6 147,077 + +0.8 147,082 + +0.6 147,141 + +0.3 147,164 + -1.8 147,212 - -0.3 147,236 - +2.5 147,288 + -0.0 147,301 + +1.4 147,301 + -0.7 147,331 + +0.2 147,339 - Atu6128 0.10 +2.0 147,339 - Atu6128 0.10 +0.5 147,469 + Atu6128 0.34 +0.3 147,515 + Atu6128 0.43 +0.7 147,525 + Atu6128 0.45 +0.1 147,535 - Atu6128 0.47 +0.6 147,535 - Atu6128 0.47 -0.1 147,536 + Atu6128 0.47 -0.3 147,627 + Atu6128 0.64 +0.4 147,651 + Atu6128 0.69 +3.1 147,673 + Atu6128 0.73 +0.2 147,749 + Atu6128 0.87 -0.5 147,757 - Atu6128 0.89 +0.7 147,768 - -1.1 147,768 - -0.2 147,783 - +0.9 147,796 + -0.2 147,803 + -0.0 147,804 - +1.3 147,806 - +0.1 147,806 - -0.7 147,835 + +0.1 147,902 + -0.3 148,005 - -1.4 148,023 - Atu6129 0.11 -0.6 148,049 - Atu6129 0.13 +0.3 148,101 - Atu6129 0.18 +1.7 148,101 - Atu6129 0.18 +2.1 148,141 + Atu6129 0.21 +0.7 148,141 + Atu6129 0.21 -0.9 148,149 - Atu6129 0.22 -1.0 148,198 - Atu6129 0.27 +0.4 148,223 + Atu6129 0.29 -2.6 148,274 + Atu6129 0.34 -0.6 148,372 + Atu6129 0.43 -0.2 148,380 - Atu6129 0.43 +0.0 148,403 + Atu6129 0.45 -0.4 148,411 - Atu6129 0.46 -0.4 148,420 + Atu6129 0.47 +0.1 148,420 + Atu6129 0.47 -0.3 148,428 - Atu6129 0.48 +1.4 148,458 + Atu6129 0.50 -1.4 148,470 + Atu6129 0.51 -1.3 148,650 - Atu6129 0.68 +2.1 148,655 + Atu6129 0.68 -0.1 148,655 + Atu6129 0.68 +0.2 148,663 - Atu6129 0.69 +0.3 148,678 + Atu6129 0.70 +1.1 148,752 - Atu6129 0.77 +0.4 148,787 + Atu6129 0.80 -0.6
Or see this region's nucleotide sequence