Experiment: Wcs_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11545 and PS417_11550 are separated by 14 nucleotides PS417_11550 and PS417_11555 overlap by 4 nucleotides
PS417_11545: PS417_11545 - spermidine/putrescine ABC transporter substrate-binding protein, at 2,482,098 to 2,483,129
_11545
PS417_11550: PS417_11550 - FAD-binding oxidoreductase, at 2,483,144 to 2,484,241
_11550
PS417_11555: PS417_11555 - (2Fe-2S)-binding protein, at 2,484,238 to 2,485,581
_11555
Position (kb)
2483
2484
2485 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2482.402 kb on + strand, within PS417_11545 at 2482.741 kb on + strand, within PS417_11545 at 2482.742 kb on - strand, within PS417_11545 at 2482.867 kb on + strand, within PS417_11545 at 2483.117 kb on + strand at 2483.511 kb on + strand, within PS417_11550 at 2483.511 kb on + strand, within PS417_11550 at 2483.599 kb on + strand, within PS417_11550 at 2484.003 kb on + strand, within PS417_11550 at 2484.071 kb on + strand at 2484.071 kb on + strand, within PS417_11550 at 2484.072 kb on - strand, within PS417_11550 at 2484.111 kb on + strand, within PS417_11550 at 2484.224 kb on + strand at 2484.708 kb on - strand, within PS417_11555 at 2484.968 kb on + strand, within PS417_11555 at 2484.969 kb on - strand, within PS417_11555 at 2485.100 kb on + strand, within PS417_11555 at 2485.190 kb on + strand, within PS417_11555
Per-strain Table
Position Strand Gene LocusTag Fraction Wcs_1 remove 2,482,402 + PS417_11545 0.29 +0.1 2,482,741 + PS417_11545 0.62 -0.5 2,482,742 - PS417_11545 0.62 +1.4 2,482,867 + PS417_11545 0.75 -1.7 2,483,117 + +1.6 2,483,511 + PS417_11550 0.33 +0.1 2,483,511 + PS417_11550 0.33 -0.2 2,483,599 + PS417_11550 0.41 -1.6 2,484,003 + PS417_11550 0.78 -0.1 2,484,071 + -0.7 2,484,071 + PS417_11550 0.84 -1.6 2,484,072 - PS417_11550 0.85 -1.3 2,484,111 + PS417_11550 0.88 -0.1 2,484,224 + -0.4 2,484,708 - PS417_11555 0.35 +0.9 2,484,968 + PS417_11555 0.54 +0.0 2,484,969 - PS417_11555 0.54 -1.0 2,485,100 + PS417_11555 0.64 -2.9 2,485,190 + PS417_11555 0.71 -2.5
Or see this region's nucleotide sequence