Strain Fitness in Pseudomonas putida KT2440 around PP_2771

Experiment: Supernatant; Burkholderia bryophila 376MFSha3.1 spent media 0.5x and Ferric chloride 1 uM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Supernatant; Burkholderia bryophila 376MFSha3.1 spent media 0.5x and Ferric chloride 1 uM
3,154,223	+		PP_2770	0.32	-2.2
3,154,224	-		PP_2770	0.32	+0.5
3,154,224	-		PP_2770	0.32	-0.0
3,154,261	-		PP_2770	0.35	-0.8
3,154,398	-		PP_2770	0.45	-0.2
3,154,398	-		PP_2770	0.45	-0.3
3,154,754	+		PP_2770	0.72	+0.8
3,154,757	+		PP_2770	0.72	+0.1
3,154,758	-		PP_2770	0.72	+0.7
3,154,758	-		PP_2770	0.72	-0.1
3,154,758	-		PP_2770	0.72	+0.1
3,154,935	+		PP_2770	0.86	+0.2
3,155,072	+				+0.0
3,155,072	+				-0.9
3,155,072	+				-0.0
3,155,090	-				-0.1
3,155,093	-				-1.4
3,155,168	+				-0.4
3,155,169	-				+0.3
3,155,169	-				+1.2
3,155,331	-		PP_2771	0.16	+0.4
3,155,569	-		PP_2771	0.37	-0.6
3,155,569	-		PP_2771	0.37	-0.3
3,155,607	-		PP_2771	0.41	-1.4
3,155,617	-		PP_2771	0.41	-0.6
3,155,695	-		PP_2771	0.49	-0.6
3,155,725	-		PP_2771	0.51	-0.5
3,155,727	+		PP_2771	0.51	+0.4
3,155,727	+		PP_2771	0.51	-0.6
3,155,728	-		PP_2771	0.52	-1.5
3,155,728	-		PP_2771	0.52	-0.1
3,156,077	+		PP_2771	0.83	+1.1
3,156,077	+		PP_2771	0.83	-0.5
3,156,077	+		PP_2771	0.83	-0.3
3,156,078	-		PP_2771	0.83	-1.5
3,156,272	+				+0.3
3,156,272	+				+0.9
3,156,279	-				+0.5
3,156,384	-				-2.4
3,156,621	-		PP_2772	0.29	+0.6
3,156,635	+		PP_2772	0.31	+0.1
3,156,636	-		PP_2772	0.31	-0.6
3,156,636	-		PP_2772	0.31	-0.3
3,156,636	-		PP_2772	0.31	+0.4
3,156,636	-		PP_2772	0.31	+2.7
3,156,733	-		PP_2772	0.39	+0.2
3,157,066	+		PP_2772	0.67	-0.2
3,157,067	-		PP_2772	0.67	-0.3
3,157,171	+		PP_2772	0.76	-0.2