Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppk and ppx are separated by 4 nucleotides ppx and yfgF are separated by 38 nucleotides
b2501: ppk - polyphosphate kinase (NCBI), at 2,621,066 to 2,623,132
ppk
b2502: ppx - exopolyphosphatase (NCBI), at 2,623,137 to 2,624,678
ppx
b2503: yfgF - predicted inner membrane protein (NCBI), at 2,624,717 to 2,626,960
yfgF
Position (kb)
2623
2624
2625 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2622.137 kb on - strand, within ppk at 2622.151 kb on - strand, within ppk at 2622.151 kb on - strand, within ppk at 2622.248 kb on - strand, within ppk at 2622.268 kb on - strand, within ppk at 2622.324 kb on + strand, within ppk at 2622.329 kb on + strand, within ppk at 2622.345 kb on + strand, within ppk at 2622.388 kb on - strand, within ppk at 2622.461 kb on + strand, within ppk at 2622.461 kb on + strand, within ppk at 2622.471 kb on - strand, within ppk at 2622.550 kb on - strand, within ppk at 2622.628 kb on - strand, within ppk at 2622.647 kb on + strand, within ppk at 2622.647 kb on + strand, within ppk at 2622.667 kb on - strand, within ppk at 2622.667 kb on - strand, within ppk at 2622.702 kb on - strand, within ppk at 2622.702 kb on - strand, within ppk at 2622.720 kb on - strand, within ppk at 2622.729 kb on - strand, within ppk at 2622.782 kb on - strand, within ppk at 2622.839 kb on - strand, within ppk at 2622.897 kb on - strand, within ppk at 2622.923 kb on - strand, within ppk at 2623.010 kb on + strand at 2623.037 kb on - strand at 2623.112 kb on - strand at 2623.112 kb on - strand at 2623.135 kb on - strand at 2623.171 kb on + strand at 2623.220 kb on - strand at 2623.220 kb on - strand at 2623.304 kb on - strand, within ppx at 2623.395 kb on - strand, within ppx at 2623.398 kb on - strand, within ppx at 2623.429 kb on + strand, within ppx at 2623.532 kb on - strand, within ppx at 2623.677 kb on + strand, within ppx at 2623.677 kb on + strand, within ppx at 2623.720 kb on + strand, within ppx at 2623.720 kb on + strand, within ppx at 2623.755 kb on + strand, within ppx at 2623.755 kb on + strand, within ppx at 2623.756 kb on - strand, within ppx at 2623.769 kb on - strand, within ppx at 2623.769 kb on - strand, within ppx at 2623.941 kb on + strand, within ppx at 2623.941 kb on + strand, within ppx at 2623.942 kb on - strand, within ppx at 2623.996 kb on + strand, within ppx at 2624.019 kb on - strand, within ppx at 2624.040 kb on - strand at 2624.040 kb on - strand, within ppx at 2624.119 kb on - strand, within ppx at 2624.127 kb on + strand, within ppx at 2624.147 kb on + strand, within ppx at 2624.147 kb on + strand, within ppx at 2624.155 kb on - strand, within ppx at 2624.248 kb on + strand, within ppx at 2624.431 kb on + strand, within ppx at 2624.441 kb on + strand, within ppx at 2624.491 kb on + strand, within ppx at 2624.539 kb on - strand at 2624.539 kb on - strand at 2624.578 kb on - strand at 2624.579 kb on + strand at 2624.579 kb on + strand at 2624.601 kb on + strand at 2624.601 kb on + strand at 2624.797 kb on - strand at 2624.821 kb on - strand at 2624.821 kb on - strand at 2624.860 kb on - strand at 2625.005 kb on - strand, within yfgF at 2625.087 kb on - strand, within yfgF at 2625.092 kb on - strand, within yfgF at 2625.197 kb on + strand, within yfgF at 2625.206 kb on - strand, within yfgF at 2625.235 kb on + strand, within yfgF at 2625.412 kb on - strand, within yfgF at 2625.412 kb on - strand, within yfgF at 2625.545 kb on + strand, within yfgF at 2625.603 kb on - strand, within yfgF
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,622,137 - ppk b2501 0.52 -3.6 2,622,151 - ppk b2501 0.52 -2.3 2,622,151 - ppk b2501 0.52 -1.7 2,622,248 - ppk b2501 0.57 -3.6 2,622,268 - ppk b2501 0.58 -2.3 2,622,324 + ppk b2501 0.61 -3.8 2,622,329 + ppk b2501 0.61 -1.8 2,622,345 + ppk b2501 0.62 -3.8 2,622,388 - ppk b2501 0.64 -1.8 2,622,461 + ppk b2501 0.67 -2.5 2,622,461 + ppk b2501 0.67 -2.2 2,622,471 - ppk b2501 0.68 -4.1 2,622,550 - ppk b2501 0.72 -3.1 2,622,628 - ppk b2501 0.76 -2.2 2,622,647 + ppk b2501 0.76 -2.7 2,622,647 + ppk b2501 0.76 -1.4 2,622,667 - ppk b2501 0.77 -2.4 2,622,667 - ppk b2501 0.77 -2.5 2,622,702 - ppk b2501 0.79 -1.6 2,622,702 - ppk b2501 0.79 -2.5 2,622,720 - ppk b2501 0.80 -2.4 2,622,729 - ppk b2501 0.80 -3.4 2,622,782 - ppk b2501 0.83 -3.9 2,622,839 - ppk b2501 0.86 -2.5 2,622,897 - ppk b2501 0.89 -2.8 2,622,923 - ppk b2501 0.90 -4.0 2,623,010 + -4.2 2,623,037 - -3.3 2,623,112 - -0.4 2,623,112 - -0.2 2,623,135 - +0.0 2,623,171 + +0.7 2,623,220 - +0.3 2,623,220 - +2.4 2,623,304 - ppx b2502 0.11 +0.9 2,623,395 - ppx b2502 0.17 +1.8 2,623,398 - ppx b2502 0.17 +0.6 2,623,429 + ppx b2502 0.19 +1.0 2,623,532 - ppx b2502 0.26 +1.1 2,623,677 + ppx b2502 0.35 +2.4 2,623,677 + ppx b2502 0.35 +0.7 2,623,720 + ppx b2502 0.38 +1.0 2,623,720 + ppx b2502 0.38 +1.1 2,623,755 + ppx b2502 0.40 +1.3 2,623,755 + ppx b2502 0.40 +1.1 2,623,756 - ppx b2502 0.40 +2.4 2,623,769 - ppx b2502 0.41 +2.2 2,623,769 - ppx b2502 0.41 +0.2 2,623,941 + ppx b2502 0.52 +1.4 2,623,941 + ppx b2502 0.52 +1.3 2,623,942 - ppx b2502 0.52 +1.5 2,623,996 + ppx b2502 0.56 +1.7 2,624,019 - ppx b2502 0.57 -0.0 2,624,040 - +2.6 2,624,040 - ppx b2502 0.59 +2.4 2,624,119 - ppx b2502 0.64 +1.3 2,624,127 + ppx b2502 0.64 +1.5 2,624,147 + ppx b2502 0.65 -1.0 2,624,147 + ppx b2502 0.65 +0.1 2,624,155 - ppx b2502 0.66 +1.1 2,624,248 + ppx b2502 0.72 +0.9 2,624,431 + ppx b2502 0.84 +1.7 2,624,441 + ppx b2502 0.85 +0.5 2,624,491 + ppx b2502 0.88 +1.3 2,624,539 - +1.2 2,624,539 - +2.0 2,624,578 - +1.1 2,624,579 + +0.8 2,624,579 + +1.3 2,624,601 + +1.6 2,624,601 + +1.5 2,624,797 - -0.3 2,624,821 - +0.7 2,624,821 - -0.4 2,624,860 - +0.3 2,625,005 - yfgF b2503 0.13 +0.3 2,625,087 - yfgF b2503 0.16 -0.1 2,625,092 - yfgF b2503 0.17 -0.3 2,625,197 + yfgF b2503 0.21 +1.0 2,625,206 - yfgF b2503 0.22 -1.8 2,625,235 + yfgF b2503 0.23 -0.1 2,625,412 - yfgF b2503 0.31 -1.0 2,625,412 - yfgF b2503 0.31 +0.4 2,625,545 + yfgF b2503 0.37 +0.4 2,625,603 - yfgF b2503 0.39 -0.5
Or see this region's nucleotide sequence