Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt emrD and yidF are separated by 7 nucleotides yidF and yidG overlap by 4 nucleotides yidG and yidH overlap by 11 nucleotides yidH and yidI are separated by 107 nucleotides yidI and yidJ are separated by 46 nucleotides
b3673: emrD - 2-module integral membrane pump; multidrug resistance (VIMSS), at 3,851,945 to 3,853,129
emrD
b3674: yidF - predicted DNA-binding transcriptional regulator (NCBI), at 3,853,137 to 3,853,634
yidF
b3675: yidG - predicted inner membrane protein (NCBI), at 3,853,631 to 3,853,993
yidG
b3676: yidH - conserved inner membrane protein (NCBI), at 3,853,983 to 3,854,330
yidH
b3677: yidI - predicted inner membrane protein (NCBI), at 3,854,438 to 3,854,887
yidI
b3678: yidJ - predicted sulfatase/phosphatase (NCBI), at 3,854,934 to 3,856,427
yidJ
Position (kb)
3853
3854
3855 Strain fitness (log2 ratio)
-2
-1
0
1 at 3853.003 kb on - strand, within emrD at 3853.023 kb on - strand at 3853.025 kb on + strand at 3853.035 kb on + strand at 3853.035 kb on + strand at 3853.042 kb on - strand at 3853.043 kb on - strand at 3853.043 kb on - strand at 3853.078 kb on + strand at 3853.078 kb on + strand at 3853.078 kb on + strand at 3853.086 kb on - strand at 3853.114 kb on + strand at 3853.133 kb on + strand at 3853.147 kb on + strand at 3853.147 kb on + strand at 3853.158 kb on - strand at 3853.160 kb on + strand at 3853.160 kb on - strand at 3853.160 kb on - strand at 3853.168 kb on - strand at 3853.178 kb on + strand at 3853.178 kb on + strand at 3853.216 kb on - strand, within yidF at 3853.216 kb on - strand, within yidF at 3853.233 kb on + strand, within yidF at 3853.238 kb on + strand, within yidF at 3853.257 kb on + strand, within yidF at 3853.257 kb on + strand, within yidF at 3853.263 kb on - strand, within yidF at 3853.265 kb on - strand, within yidF at 3853.284 kb on - strand, within yidF at 3853.295 kb on - strand, within yidF at 3853.295 kb on - strand, within yidF at 3853.378 kb on + strand, within yidF at 3853.390 kb on - strand, within yidF at 3853.390 kb on - strand, within yidF at 3853.393 kb on + strand, within yidF at 3853.399 kb on + strand, within yidF at 3853.399 kb on + strand, within yidF at 3853.407 kb on - strand, within yidF at 3853.418 kb on - strand, within yidF at 3853.419 kb on - strand, within yidF at 3853.419 kb on - strand, within yidF at 3853.419 kb on - strand, within yidF at 3853.419 kb on - strand, within yidF at 3853.445 kb on + strand, within yidF at 3853.445 kb on + strand, within yidF at 3853.445 kb on + strand, within yidF at 3853.450 kb on + strand, within yidF at 3853.453 kb on - strand, within yidF at 3853.453 kb on - strand, within yidF at 3853.453 kb on - strand, within yidF at 3853.501 kb on + strand, within yidF at 3853.501 kb on + strand, within yidF at 3853.506 kb on + strand, within yidF at 3853.511 kb on + strand, within yidF at 3853.511 kb on + strand, within yidF at 3853.511 kb on + strand, within yidF at 3853.597 kb on - strand at 3853.597 kb on - strand at 3853.612 kb on + strand at 3853.612 kb on + strand at 3853.666 kb on + strand at 3853.687 kb on + strand, within yidG at 3853.695 kb on + strand, within yidG at 3853.697 kb on + strand, within yidG at 3853.703 kb on - strand, within yidG at 3853.727 kb on + strand, within yidG at 3853.727 kb on + strand, within yidG at 3853.781 kb on - strand, within yidG at 3853.781 kb on - strand, within yidG at 3853.796 kb on - strand, within yidG at 3853.796 kb on - strand, within yidG at 3853.819 kb on - strand, within yidG at 3853.869 kb on - strand, within yidG at 3853.904 kb on + strand, within yidG at 3853.904 kb on + strand, within yidG at 3853.914 kb on + strand, within yidG at 3853.919 kb on + strand, within yidG at 3853.933 kb on - strand, within yidG at 3853.933 kb on - strand, within yidG at 3853.959 kb on - strand at 3853.959 kb on - strand at 3853.993 kb on + strand at 3854.005 kb on - strand at 3854.011 kb on - strand at 3854.011 kb on - strand at 3854.053 kb on - strand, within yidH at 3854.062 kb on + strand, within yidH at 3854.062 kb on + strand, within yidH at 3854.062 kb on + strand, within yidH at 3854.080 kb on + strand, within yidH at 3854.080 kb on + strand, within yidH at 3854.129 kb on - strand, within yidH at 3854.214 kb on - strand, within yidH at 3854.214 kb on - strand, within yidH at 3854.251 kb on - strand, within yidH at 3854.251 kb on - strand, within yidH at 3854.305 kb on + strand at 3854.356 kb on + strand at 3854.357 kb on + strand at 3854.357 kb on + strand at 3854.445 kb on + strand at 3854.445 kb on - strand at 3854.448 kb on - strand at 3854.448 kb on - strand at 3854.450 kb on - strand at 3854.562 kb on + strand, within yidI at 3854.562 kb on + strand, within yidI at 3854.575 kb on - strand, within yidI at 3854.595 kb on - strand, within yidI at 3854.693 kb on - strand, within yidI at 3854.705 kb on - strand, within yidI at 3854.705 kb on - strand, within yidI at 3854.708 kb on - strand, within yidI at 3854.743 kb on - strand, within yidI at 3854.770 kb on + strand, within yidI at 3854.803 kb on - strand, within yidI at 3854.803 kb on - strand, within yidI at 3854.889 kb on - strand at 3854.889 kb on - strand at 3854.908 kb on - strand at 3854.932 kb on + strand at 3854.997 kb on + strand at 3855.018 kb on + strand at 3855.018 kb on + strand at 3855.049 kb on - strand at 3855.053 kb on - strand at 3855.053 kb on - strand at 3855.064 kb on - strand at 3855.064 kb on - strand at 3855.072 kb on - strand at 3855.072 kb on - strand at 3855.132 kb on - strand, within yidJ at 3855.265 kb on - strand, within yidJ at 3855.265 kb on - strand, within yidJ at 3855.307 kb on - strand, within yidJ at 3855.327 kb on - strand, within yidJ
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 3,853,003 - emrD b3673 0.89 +0.9 3,853,023 - -0.2 3,853,025 + -0.1 3,853,035 + +0.1 3,853,035 + -0.4 3,853,042 - -1.2 3,853,043 - +0.7 3,853,043 - -0.5 3,853,078 + -0.6 3,853,078 + +0.9 3,853,078 + -0.7 3,853,086 - +0.7 3,853,114 + -0.5 3,853,133 + -0.5 3,853,147 + -1.9 3,853,147 + -0.4 3,853,158 - -0.1 3,853,160 + -0.5 3,853,160 - +0.4 3,853,160 - -1.1 3,853,168 - -1.2 3,853,178 + +0.1 3,853,178 + -1.1 3,853,216 - yidF b3674 0.16 -0.8 3,853,216 - yidF b3674 0.16 +0.8 3,853,233 + yidF b3674 0.19 +0.1 3,853,238 + yidF b3674 0.20 +0.1 3,853,257 + yidF b3674 0.24 +0.5 3,853,257 + yidF b3674 0.24 -0.2 3,853,263 - yidF b3674 0.25 -0.2 3,853,265 - yidF b3674 0.26 -0.6 3,853,284 - yidF b3674 0.30 +0.5 3,853,295 - yidF b3674 0.32 +0.1 3,853,295 - yidF b3674 0.32 +0.3 3,853,378 + yidF b3674 0.48 -0.3 3,853,390 - yidF b3674 0.51 +0.3 3,853,390 - yidF b3674 0.51 -0.4 3,853,393 + yidF b3674 0.51 -0.1 3,853,399 + yidF b3674 0.53 -0.1 3,853,399 + yidF b3674 0.53 -0.1 3,853,407 - yidF b3674 0.54 -0.5 3,853,418 - yidF b3674 0.56 +0.4 3,853,419 - yidF b3674 0.57 +0.3 3,853,419 - yidF b3674 0.57 -0.2 3,853,419 - yidF b3674 0.57 -0.5 3,853,419 - yidF b3674 0.57 +0.4 3,853,445 + yidF b3674 0.62 -0.2 3,853,445 + yidF b3674 0.62 +0.1 3,853,445 + yidF b3674 0.62 -0.6 3,853,450 + yidF b3674 0.63 +0.2 3,853,453 - yidF b3674 0.63 -0.2 3,853,453 - yidF b3674 0.63 +0.1 3,853,453 - yidF b3674 0.63 -0.6 3,853,501 + yidF b3674 0.73 +0.8 3,853,501 + yidF b3674 0.73 -0.8 3,853,506 + yidF b3674 0.74 -0.3 3,853,511 + yidF b3674 0.75 +1.3 3,853,511 + yidF b3674 0.75 +0.2 3,853,511 + yidF b3674 0.75 -0.1 3,853,597 - -0.3 3,853,597 - +1.3 3,853,612 + +1.2 3,853,612 + -0.3 3,853,666 + -0.3 3,853,687 + yidG b3675 0.15 +0.6 3,853,695 + yidG b3675 0.18 -0.3 3,853,697 + yidG b3675 0.18 +1.2 3,853,703 - yidG b3675 0.20 +0.2 3,853,727 + yidG b3675 0.26 +0.1 3,853,727 + yidG b3675 0.26 +0.2 3,853,781 - yidG b3675 0.41 -0.0 3,853,781 - yidG b3675 0.41 -0.2 3,853,796 - yidG b3675 0.45 +0.2 3,853,796 - yidG b3675 0.45 -2.0 3,853,819 - yidG b3675 0.52 +0.4 3,853,869 - yidG b3675 0.66 -1.2 3,853,904 + yidG b3675 0.75 +0.8 3,853,904 + yidG b3675 0.75 -0.2 3,853,914 + yidG b3675 0.78 +0.8 3,853,919 + yidG b3675 0.79 +0.0 3,853,933 - yidG b3675 0.83 -0.5 3,853,933 - yidG b3675 0.83 -0.1 3,853,959 - -0.5 3,853,959 - +1.4 3,853,993 + -0.1 3,854,005 - -0.7 3,854,011 - -0.1 3,854,011 - +0.1 3,854,053 - yidH b3676 0.20 -0.2 3,854,062 + yidH b3676 0.23 -0.4 3,854,062 + yidH b3676 0.23 -1.2 3,854,062 + yidH b3676 0.23 -0.1 3,854,080 + yidH b3676 0.28 -0.5 3,854,080 + yidH b3676 0.28 +0.2 3,854,129 - yidH b3676 0.42 -1.2 3,854,214 - yidH b3676 0.66 -0.2 3,854,214 - yidH b3676 0.66 +0.9 3,854,251 - yidH b3676 0.77 -1.4 3,854,251 - yidH b3676 0.77 +0.2 3,854,305 + -0.6 3,854,356 + -0.9 3,854,357 + -0.0 3,854,357 + -1.0 3,854,445 + +1.4 3,854,445 - +0.9 3,854,448 - +1.4 3,854,448 - +0.2 3,854,450 - -0.1 3,854,562 + yidI b3677 0.28 -0.0 3,854,562 + yidI b3677 0.28 -0.8 3,854,575 - yidI b3677 0.30 -0.1 3,854,595 - yidI b3677 0.35 +0.2 3,854,693 - yidI b3677 0.57 +0.5 3,854,705 - yidI b3677 0.59 -0.2 3,854,705 - yidI b3677 0.59 -0.2 3,854,708 - yidI b3677 0.60 -0.8 3,854,743 - yidI b3677 0.68 -0.6 3,854,770 + yidI b3677 0.74 +0.5 3,854,803 - yidI b3677 0.81 -0.1 3,854,803 - yidI b3677 0.81 +0.4 3,854,889 - -0.1 3,854,889 - -0.6 3,854,908 - +0.2 3,854,932 + -0.6 3,854,997 + +0.2 3,855,018 + +0.3 3,855,018 + -0.3 3,855,049 - -0.0 3,855,053 - +0.5 3,855,053 - -0.7 3,855,064 - -1.1 3,855,064 - -0.4 3,855,072 - +0.6 3,855,072 - -0.1 3,855,132 - yidJ b3678 0.13 +0.4 3,855,265 - yidJ b3678 0.22 -0.5 3,855,265 - yidJ b3678 0.22 +1.2 3,855,307 - yidJ b3678 0.25 -1.1 3,855,327 - yidJ b3678 0.26 -0.2
Or see this region's nucleotide sequence