Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt murG and murC are separated by 53 nucleotides murC and ddlB overlap by 8 nucleotides ddlB and ftsQ are separated by 1 nucleotides
b0090: murG - N-acetylglucosaminyl transferase (NCBI), at 99,644 to 100,711
murG
b0091: murC - UDP-N-acetylmuramate--L-alanine ligase (NCBI), at 100,765 to 102,240
murC
b0092: ddlB - D-alanylalanine synthetase (NCBI), at 102,233 to 103,153
ddlB
b0093: ftsQ - membrane anchored protein involved in growth of wall at septum (NCBI), at 103,155 to 103,985
ftsQ
Position (kb) 100
101
102
103 Strain fitness (log2 ratio) -1
0
1
2 at 100.705 kb on + strand at 100.709 kb on + strand at 100.709 kb on + strand at 102.254 kb on + strand at 102.265 kb on + strand at 102.265 kb on + strand at 102.295 kb on + strand at 102.446 kb on + strand, within ddlB at 102.492 kb on + strand, within ddlB at 102.492 kb on + strand, within ddlB at 102.496 kb on + strand, within ddlB at 102.498 kb on + strand, within ddlB at 102.573 kb on + strand, within ddlB at 102.573 kb on + strand, within ddlB at 102.573 kb on + strand, within ddlB at 102.793 kb on + strand, within ddlB at 102.829 kb on + strand, within ddlB at 102.984 kb on + strand, within ddlB at 103.002 kb on + strand, within ddlB at 103.017 kb on + strand, within ddlB at 103.068 kb on + strand at 103.138 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 100,705 + +0.2 100,709 + +0.7 100,709 + +2.5 102,254 + +0.8 102,265 + +0.4 102,265 + +1.9 102,295 + +1.0 102,446 + ddlB b0092 0.23 +2.1 102,492 + ddlB b0092 0.28 +1.4 102,492 + ddlB b0092 0.28 +1.6 102,496 + ddlB b0092 0.29 +2.6 102,498 + ddlB b0092 0.29 +0.7 102,573 + ddlB b0092 0.37 +1.4 102,573 + ddlB b0092 0.37 +1.2 102,573 + ddlB b0092 0.37 +2.2 102,793 + ddlB b0092 0.61 +1.4 102,829 + ddlB b0092 0.65 +2.1 102,984 + ddlB b0092 0.82 +1.9 103,002 + ddlB b0092 0.83 +1.1 103,017 + ddlB b0092 0.85 +1.6 103,068 + +1.5 103,138 + -0.7
Or see this region's nucleotide sequence