Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yebZ and yobA are separated by 3 nucleotides yobA and holE are separated by 138 nucleotides holE and yobB are separated by 101 nucleotides yobB and exoX are separated by 23 nucleotides exoX and ptrB overlap by 4 nucleotides
b1840: yebZ - predicted inner membrane protein (NCBI), at 1,921,743 to 1,922,615
yebZ
b1841: yobA - hypothetical protein (NCBI), at 1,922,619 to 1,922,993
yobA
b1842: holE - DNA polymerase III, theta subunit (NCBI), at 1,923,132 to 1,923,362
holE
b1843: yobB - hypothetical protein (NCBI), at 1,923,464 to 1,924,120
yobB
b1844: exoX - exodeoxyribonuclease X (NCBI), at 1,924,144 to 1,924,806
exoX
b1845: ptrB - protease II (NCBI), at 1,924,803 to 1,926,863
ptrB
Position (kb)
1923
1924
1925 Strain fitness (log2 ratio)
-2
-1
0
1 at 1922.482 kb on - strand, within yebZ at 1922.528 kb on + strand at 1922.536 kb on - strand at 1922.642 kb on - strand at 1922.681 kb on + strand, within yobA at 1922.681 kb on + strand, within yobA at 1923.028 kb on + strand at 1923.059 kb on - strand at 1923.246 kb on - strand, within holE at 1923.246 kb on - strand, within holE at 1923.296 kb on + strand, within holE at 1923.364 kb on + strand at 1923.364 kb on + strand at 1923.493 kb on + strand at 1923.493 kb on + strand at 1923.666 kb on - strand, within yobB at 1923.831 kb on - strand, within yobB at 1923.840 kb on - strand, within yobB at 1923.840 kb on - strand, within yobB at 1923.846 kb on + strand, within yobB at 1923.995 kb on + strand, within yobB at 1924.003 kb on - strand, within yobB at 1924.274 kb on + strand, within exoX at 1924.274 kb on + strand, within exoX at 1924.274 kb on - strand, within exoX at 1924.309 kb on - strand, within exoX at 1924.361 kb on - strand, within exoX at 1924.361 kb on - strand, within exoX at 1924.423 kb on + strand, within exoX at 1924.423 kb on + strand, within exoX at 1924.471 kb on + strand, within exoX at 1924.507 kb on - strand, within exoX at 1924.609 kb on - strand, within exoX at 1924.715 kb on + strand, within exoX at 1924.770 kb on + strand at 1924.831 kb on - strand at 1924.893 kb on + strand at 1925.014 kb on - strand, within ptrB
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,922,482 - yebZ b1840 0.85 +0.7 1,922,528 + -0.8 1,922,536 - +0.9 1,922,642 - -0.0 1,922,681 + yobA b1841 0.17 -0.5 1,922,681 + yobA b1841 0.17 -0.5 1,923,028 + -0.1 1,923,059 - +0.1 1,923,246 - holE b1842 0.49 -1.9 1,923,246 - holE b1842 0.49 -0.5 1,923,296 + holE b1842 0.71 -0.5 1,923,364 + +0.5 1,923,364 + +0.2 1,923,493 + -0.2 1,923,493 + -0.0 1,923,666 - yobB b1843 0.31 +0.1 1,923,831 - yobB b1843 0.56 -0.1 1,923,840 - yobB b1843 0.57 -0.2 1,923,840 - yobB b1843 0.57 -0.2 1,923,846 + yobB b1843 0.58 -0.4 1,923,995 + yobB b1843 0.81 +0.0 1,924,003 - yobB b1843 0.82 -0.0 1,924,274 + exoX b1844 0.20 +0.5 1,924,274 + exoX b1844 0.20 -0.3 1,924,274 - exoX b1844 0.20 +0.1 1,924,309 - exoX b1844 0.25 -0.3 1,924,361 - exoX b1844 0.33 +0.5 1,924,361 - exoX b1844 0.33 +0.5 1,924,423 + exoX b1844 0.42 -0.7 1,924,423 + exoX b1844 0.42 +0.5 1,924,471 + exoX b1844 0.49 -0.0 1,924,507 - exoX b1844 0.55 -0.8 1,924,609 - exoX b1844 0.70 +0.2 1,924,715 + exoX b1844 0.86 +0.3 1,924,770 + +0.8 1,924,831 - +0.2 1,924,893 + -0.1 1,925,014 - ptrB b1845 0.10 -0.7
Or see this region's nucleotide sequence