Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yciX_1 and yciX are separated by 2 nucleotides yciX and acnA are separated by 372 nucleotides acnA and ribA are separated by 63 nucleotides ribA and pgpB are separated by 169 nucleotides
b4522: yciX_1 - hypothetical protein (NCBI), at 1,333,184 to 1,333,312
_1
b4523: yciX - hypothetical protein (RefSeq), at 1,333,315 to 1,333,482
yciX
b1276: acnA - aconitate hydratase (NCBI), at 1,333,855 to 1,336,530
acnA
b1277: ribA - GTP cyclohydrolase II protein (NCBI), at 1,336,594 to 1,337,184
ribA
b1278: pgpB - phosphatidylglycerophosphatase B (NCBI), at 1,337,354 to 1,338,118
pgpB
Position (kb)
1333
1334
1335
1336
1337 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1332.922 kb on + strand at 1332.930 kb on - strand at 1332.930 kb on - strand at 1333.156 kb on + strand at 1333.158 kb on - strand at 1333.160 kb on - strand at 1333.197 kb on + strand, within yciX_1 at 1333.198 kb on + strand, within yciX_1 at 1333.275 kb on - strand, within yciX_1 at 1333.286 kb on - strand, within yciX_1 at 1333.286 kb on - strand, within yciX_1 at 1333.301 kb on + strand at 1333.301 kb on + strand at 1333.311 kb on + strand at 1333.320 kb on - strand at 1333.320 kb on - strand at 1333.322 kb on + strand at 1333.322 kb on + strand at 1333.345 kb on + strand, within yciX at 1333.345 kb on + strand, within yciX at 1333.345 kb on + strand, within yciX at 1333.349 kb on + strand, within yciX at 1333.418 kb on + strand, within yciX at 1333.420 kb on - strand, within yciX at 1333.434 kb on - strand, within yciX at 1333.450 kb on - strand, within yciX at 1333.450 kb on - strand, within yciX at 1333.525 kb on + strand at 1333.538 kb on + strand at 1333.578 kb on + strand at 1333.606 kb on - strand at 1333.606 kb on - strand at 1333.606 kb on - strand at 1333.606 kb on - strand at 1333.630 kb on - strand at 1333.655 kb on + strand at 1333.670 kb on + strand at 1333.670 kb on + strand at 1333.678 kb on - strand at 1333.794 kb on + strand at 1333.794 kb on + strand at 1333.818 kb on + strand at 1333.838 kb on + strand at 1333.841 kb on - strand at 1333.847 kb on - strand at 1333.873 kb on - strand at 1333.932 kb on - strand at 1334.080 kb on - strand at 1334.080 kb on - strand at 1334.663 kb on - strand, within acnA at 1334.824 kb on - strand, within acnA at 1334.824 kb on - strand, within acnA at 1334.962 kb on + strand, within acnA at 1335.010 kb on + strand, within acnA at 1335.010 kb on + strand, within acnA at 1335.167 kb on + strand, within acnA at 1335.181 kb on + strand, within acnA at 1335.573 kb on - strand, within acnA at 1335.601 kb on - strand, within acnA at 1335.601 kb on - strand, within acnA at 1335.669 kb on + strand, within acnA at 1335.669 kb on + strand, within acnA at 1335.825 kb on - strand, within acnA at 1335.825 kb on - strand, within acnA at 1335.902 kb on - strand, within acnA at 1335.902 kb on - strand, within acnA at 1336.129 kb on - strand, within acnA at 1336.223 kb on + strand, within acnA at 1337.219 kb on - strand at 1337.219 kb on - strand at 1337.498 kb on + strand, within pgpB at 1337.503 kb on + strand, within pgpB at 1337.522 kb on - strand, within pgpB
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,332,922 + +0.5 1,332,930 - -1.1 1,332,930 - +0.7 1,333,156 + +0.8 1,333,158 - +0.5 1,333,160 - -2.3 1,333,197 + yciX_1 b4522 0.10 +0.4 1,333,198 + yciX_1 b4522 0.11 +0.3 1,333,275 - yciX_1 b4522 0.71 -1.8 1,333,286 - yciX_1 b4522 0.79 +1.2 1,333,286 - yciX_1 b4522 0.79 +1.1 1,333,301 + +0.6 1,333,301 + -0.2 1,333,311 + -0.3 1,333,320 - +0.5 1,333,320 - +1.1 1,333,322 + +0.8 1,333,322 + +0.7 1,333,345 + yciX b4523 0.18 -0.2 1,333,345 + yciX b4523 0.18 +0.8 1,333,345 + yciX b4523 0.18 +0.7 1,333,349 + yciX b4523 0.20 +0.4 1,333,418 + yciX b4523 0.61 -1.6 1,333,420 - yciX b4523 0.62 +0.5 1,333,434 - yciX b4523 0.71 +0.5 1,333,450 - yciX b4523 0.80 +0.5 1,333,450 - yciX b4523 0.80 -2.3 1,333,525 + +0.3 1,333,538 + +0.6 1,333,578 + +1.0 1,333,606 - +1.8 1,333,606 - +0.5 1,333,606 - +0.5 1,333,606 - +0.3 1,333,630 - -2.1 1,333,655 + +0.5 1,333,670 + -0.4 1,333,670 + -1.2 1,333,678 - +1.5 1,333,794 + +0.1 1,333,794 + -0.1 1,333,818 + +1.0 1,333,838 + +0.5 1,333,841 - +0.4 1,333,847 - -0.4 1,333,873 - +0.6 1,333,932 - +0.0 1,334,080 - -0.4 1,334,080 - +1.1 1,334,663 - acnA b1276 0.30 +0.5 1,334,824 - acnA b1276 0.36 -1.2 1,334,824 - acnA b1276 0.36 -1.8 1,334,962 + acnA b1276 0.41 +0.5 1,335,010 + acnA b1276 0.43 -0.8 1,335,010 + acnA b1276 0.43 -0.2 1,335,167 + acnA b1276 0.49 +0.6 1,335,181 + acnA b1276 0.50 +0.9 1,335,573 - acnA b1276 0.64 +0.7 1,335,601 - acnA b1276 0.65 -0.1 1,335,601 - acnA b1276 0.65 -0.2 1,335,669 + acnA b1276 0.68 +0.7 1,335,669 + acnA b1276 0.68 +0.5 1,335,825 - acnA b1276 0.74 -0.5 1,335,825 - acnA b1276 0.74 -0.5 1,335,902 - acnA b1276 0.76 -1.4 1,335,902 - acnA b1276 0.76 -0.6 1,336,129 - acnA b1276 0.85 +0.1 1,336,223 + acnA b1276 0.88 +0.1 1,337,219 - +1.4 1,337,219 - +0.5 1,337,498 + pgpB b1278 0.19 +0.3 1,337,503 + pgpB b1278 0.19 -0.2 1,337,522 - pgpB b1278 0.22 -2.5
Or see this region's nucleotide sequence