Strain Fitness in Escherichia coli BW25113 around b1897

Experiment: Cisplatin 0.05 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntotsA and otsB overlap by 26 nucleotidesotsB and araH are separated by 166 nucleotides b1896: otsA - trehalose-6-phosphate synthase (NCBI), at 1,978,212 to 1,979,636 otsA b1897: otsB - trehalose-6-phosphate phosphatase, biosynthetic (NCBI), at 1,979,611 to 1,980,411 otsB b4460: araH - fused L-arabinose transporter subunits of ABC superfamily: membrane components (RefSeq), at 1,980,578 to 1,981,564 araH Position (kb) 1979 1980 1981Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1978.719 kb on + strand, within otsAat 1978.728 kb on - strand, within otsAat 1978.731 kb on + strand, within otsAat 1978.731 kb on + strand, within otsAat 1978.732 kb on - strand, within otsAat 1978.732 kb on - strand, within otsAat 1978.737 kb on - strand, within otsAat 1978.737 kb on - strand, within otsAat 1978.850 kb on - strand, within otsAat 1978.850 kb on - strand, within otsAat 1978.883 kb on - strand, within otsAat 1978.971 kb on - strand, within otsAat 1978.977 kb on + strand, within otsAat 1978.997 kb on - strand, within otsAat 1978.997 kb on - strand, within otsAat 1979.007 kb on - strand, within otsAat 1979.007 kb on - strand, within otsAat 1979.019 kb on - strand, within otsAat 1979.044 kb on - strand, within otsAat 1979.066 kb on - strand, within otsAat 1979.093 kb on + strand, within otsAat 1979.093 kb on + strand, within otsAat 1979.124 kb on + strand, within otsAat 1979.219 kb on - strand, within otsAat 1979.219 kb on - strand, within otsAat 1979.250 kb on + strand, within otsAat 1979.262 kb on - strand, within otsAat 1979.268 kb on + strand, within otsAat 1979.268 kb on - strand, within otsAat 1979.268 kb on - strand, within otsAat 1979.443 kb on - strand, within otsAat 1979.748 kb on - strand, within otsBat 1979.885 kb on + strand, within otsBat 1979.998 kb on - strand, within otsBat 1980.189 kb on - strand, within otsBat 1980.229 kb on - strand, within otsBat 1980.256 kb on - strand, within otsBat 1980.274 kb on + strand, within otsBat 1980.341 kb on + strandat 1980.827 kb on - strandat 1980.984 kb on + strand, within araHat 1981.002 kb on - strand, within araHat 1981.002 kb on - strand, within araH

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml
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1,978,719 + otsA b1896 0.36 -2.1
1,978,728 - otsA b1896 0.36 -0.3
1,978,731 + otsA b1896 0.36 -0.3
1,978,731 + otsA b1896 0.36 +3.0
1,978,732 - otsA b1896 0.36 +0.1
1,978,732 - otsA b1896 0.36 -0.0
1,978,737 - otsA b1896 0.37 +0.7
1,978,737 - otsA b1896 0.37 -0.3
1,978,850 - otsA b1896 0.45 +1.0
1,978,850 - otsA b1896 0.45 -0.5
1,978,883 - otsA b1896 0.47 +0.0
1,978,971 - otsA b1896 0.53 -0.2
1,978,977 + otsA b1896 0.54 +0.2
1,978,997 - otsA b1896 0.55 -0.4
1,978,997 - otsA b1896 0.55 -0.1
1,979,007 - otsA b1896 0.56 +0.2
1,979,007 - otsA b1896 0.56 +1.0
1,979,019 - otsA b1896 0.57 -0.2
1,979,044 - otsA b1896 0.58 +0.0
1,979,066 - otsA b1896 0.60 +0.5
1,979,093 + otsA b1896 0.62 -0.1
1,979,093 + otsA b1896 0.62 -0.4
1,979,124 + otsA b1896 0.64 -1.0
1,979,219 - otsA b1896 0.71 -0.7
1,979,219 - otsA b1896 0.71 +0.5
1,979,250 + otsA b1896 0.73 -0.4
1,979,262 - otsA b1896 0.74 +0.5
1,979,268 + otsA b1896 0.74 +0.3
1,979,268 - otsA b1896 0.74 -0.2
1,979,268 - otsA b1896 0.74 -0.1
1,979,443 - otsA b1896 0.86 +0.3
1,979,748 - otsB b1897 0.17 +0.4
1,979,885 + otsB b1897 0.34 -0.1
1,979,998 - otsB b1897 0.48 +0.4
1,980,189 - otsB b1897 0.72 -0.3
1,980,229 - otsB b1897 0.77 -0.1
1,980,256 - otsB b1897 0.81 -0.8
1,980,274 + otsB b1897 0.83 +0.8
1,980,341 + +0.8
1,980,827 - +0.5
1,980,984 + araH b4460 0.41 -0.3
1,981,002 - araH b4460 0.43 +1.0
1,981,002 - araH b4460 0.43 -0.4

Or see this region's nucleotide sequence