Strain Fitness in Escherichia coli BW25113 around b0486

Experiment: copper (II) chloride 2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybaS and ybaT are separated by 2 nucleotidesybaT and cueR are separated by 124 nucleotidescueR and ybbJ are separated by 0 nucleotidesybbJ and ybbK overlap by 4 nucleotides b0485: ybaS - predicted glutaminase (NCBI), at 510,865 to 511,797 ybaS b0486: ybaT - predicted transporter (NCBI), at 511,800 to 513,092 ybaT b0487: cueR - DNA-binding transcriptional activator of copper-responsive regulon genes (NCBI), at 513,217 to 513,624 cueR b0488: ybbJ - conserved inner membrane protein (RefSeq), at 513,625 to 514,083 ybbJ b0489: ybbK - predicted protease, membrane anchored (NCBI), at 514,080 to 514,997 ybbK Position (kb) 511 512 513 514Strain fitness (log2 ratio) -2 -1 0 1at 510.844 kb on + strandat 510.903 kb on + strandat 510.903 kb on + strandat 510.979 kb on - strand, within ybaSat 510.979 kb on - strand, within ybaSat 511.000 kb on - strand, within ybaSat 511.172 kb on + strand, within ybaSat 511.172 kb on + strand, within ybaSat 511.359 kb on + strand, within ybaSat 511.389 kb on - strand, within ybaSat 511.392 kb on - strand, within ybaSat 511.434 kb on + strand, within ybaSat 511.583 kb on + strand, within ybaSat 511.583 kb on + strand, within ybaSat 511.673 kb on + strand, within ybaSat 511.673 kb on + strand, within ybaSat 511.673 kb on + strand, within ybaSat 511.980 kb on + strand, within ybaTat 512.081 kb on + strand, within ybaTat 512.081 kb on + strand, within ybaTat 512.171 kb on + strand, within ybaTat 512.239 kb on - strand, within ybaTat 512.413 kb on - strand, within ybaTat 512.414 kb on - strand, within ybaTat 512.514 kb on - strand, within ybaTat 512.567 kb on - strand, within ybaTat 512.604 kb on + strand, within ybaTat 512.771 kb on + strand, within ybaTat 512.830 kb on + strand, within ybaTat 512.830 kb on + strand, within ybaTat 512.846 kb on - strand, within ybaTat 512.886 kb on + strand, within ybaTat 512.890 kb on + strand, within ybaTat 512.953 kb on + strand, within ybaTat 512.958 kb on + strand, within ybaTat 512.958 kb on + strand, within ybaTat 512.991 kb on + strandat 512.998 kb on + strandat 512.998 kb on + strandat 513.013 kb on - strandat 513.013 kb on - strandat 513.039 kb on + strandat 513.039 kb on + strandat 513.057 kb on - strandat 513.057 kb on - strandat 513.057 kb on - strandat 513.077 kb on + strandat 513.077 kb on + strandat 513.095 kb on - strandat 513.107 kb on + strandat 513.109 kb on + strandat 513.182 kb on + strandat 513.205 kb on - strandat 513.287 kb on - strand, within cueRat 513.295 kb on - strand, within cueRat 513.431 kb on - strand, within cueRat 513.480 kb on + strand, within cueRat 513.537 kb on - strand, within cueRat 513.537 kb on - strand, within cueRat 513.700 kb on - strand, within ybbJat 513.728 kb on - strand, within ybbJat 513.742 kb on + strand, within ybbJat 513.742 kb on + strand, within ybbJat 513.949 kb on - strand, within ybbJat 513.949 kb on - strand, within ybbJat 513.958 kb on + strand, within ybbJat 513.958 kb on + strand, within ybbJat 513.977 kb on + strand, within ybbJ

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Per-strain Table

Position Strand Gene LocusTag Fraction copper (II) chloride 2 mM
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510,844 + +0.6
510,903 + -0.9
510,903 + -0.1
510,979 - ybaS b0485 0.12 +0.2
510,979 - ybaS b0485 0.12 -1.3
511,000 - ybaS b0485 0.14 +0.4
511,172 + ybaS b0485 0.33 -0.3
511,172 + ybaS b0485 0.33 -0.6
511,359 + ybaS b0485 0.53 +1.4
511,389 - ybaS b0485 0.56 +0.3
511,392 - ybaS b0485 0.56 -1.2
511,434 + ybaS b0485 0.61 -0.7
511,583 + ybaS b0485 0.77 +0.1
511,583 + ybaS b0485 0.77 +0.6
511,673 + ybaS b0485 0.87 -0.4
511,673 + ybaS b0485 0.87 -0.5
511,673 + ybaS b0485 0.87 -0.2
511,980 + ybaT b0486 0.14 +1.1
512,081 + ybaT b0486 0.22 +0.3
512,081 + ybaT b0486 0.22 +0.5
512,171 + ybaT b0486 0.29 +0.5
512,239 - ybaT b0486 0.34 -0.4
512,413 - ybaT b0486 0.47 +0.1
512,414 - ybaT b0486 0.47 -0.7
512,514 - ybaT b0486 0.55 -0.0
512,567 - ybaT b0486 0.59 +0.4
512,604 + ybaT b0486 0.62 -0.3
512,771 + ybaT b0486 0.75 +0.7
512,830 + ybaT b0486 0.80 -0.0
512,830 + ybaT b0486 0.80 +0.7
512,846 - ybaT b0486 0.81 -0.4
512,886 + ybaT b0486 0.84 -0.0
512,890 + ybaT b0486 0.84 +0.1
512,953 + ybaT b0486 0.89 -0.3
512,958 + ybaT b0486 0.90 +0.5
512,958 + ybaT b0486 0.90 +0.6
512,991 + +1.2
512,998 + +0.5
512,998 + -0.2
513,013 - -0.5
513,013 - -0.4
513,039 + -2.0
513,039 + +0.8
513,057 - +0.7
513,057 - +0.2
513,057 - -0.4
513,077 + -0.4
513,077 + +0.1
513,095 - -1.0
513,107 + +0.2
513,109 + +0.5
513,182 + +0.0
513,205 - +0.4
513,287 - cueR b0487 0.17 -2.1
513,295 - cueR b0487 0.19 -0.2
513,431 - cueR b0487 0.52 -1.0
513,480 + cueR b0487 0.64 -1.1
513,537 - cueR b0487 0.78 -0.2
513,537 - cueR b0487 0.78 -2.4
513,700 - ybbJ b0488 0.16 -0.1
513,728 - ybbJ b0488 0.22 -0.1
513,742 + ybbJ b0488 0.25 +1.3
513,742 + ybbJ b0488 0.25 -0.2
513,949 - ybbJ b0488 0.71 +0.2
513,949 - ybbJ b0488 0.71 -0.3
513,958 + ybbJ b0488 0.73 -1.3
513,958 + ybbJ b0488 0.73 -0.4
513,977 + ybbJ b0488 0.77 +0.3

Or see this region's nucleotide sequence