Strain Fitness in Escherichia coli BW25113 around b1162

Experiment: Cisplatin 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycgX and ycgE are separated by 220 nucleotidesycgE and ycgF are separated by 204 nucleotides b1161: ycgX - hypothetical protein (NCBI), at 1,211,926 to 1,212,330 ycgX b1162: ycgE - predicted DNA-binding transcriptional regulator (NCBI), at 1,212,551 to 1,213,282 ycgE b1163: ycgF - predicted FAD-binding phosphodiesterase (NCBI), at 1,213,487 to 1,214,698 ycgF Position (kb) 1212 1213 1214Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1212.255 kb on - strand, within ycgXat 1212.292 kb on - strandat 1212.292 kb on - strandat 1212.327 kb on - strandat 1212.328 kb on - strandat 1212.345 kb on - strandat 1212.371 kb on - strandat 1212.371 kb on - strandat 1212.376 kb on - strandat 1212.376 kb on - strandat 1212.425 kb on - strandat 1212.427 kb on + strandat 1212.435 kb on - strandat 1212.435 kb on - strandat 1212.472 kb on + strandat 1212.477 kb on + strandat 1212.477 kb on + strandat 1212.483 kb on + strandat 1212.483 kb on + strandat 1212.579 kb on - strandat 1212.579 kb on - strandat 1212.596 kb on - strandat 1212.618 kb on + strandat 1212.689 kb on - strand, within ycgEat 1212.689 kb on - strand, within ycgEat 1212.803 kb on + strand, within ycgEat 1212.864 kb on - strand, within ycgEat 1212.892 kb on - strand, within ycgEat 1212.894 kb on + strand, within ycgEat 1212.951 kb on - strand, within ycgEat 1212.954 kb on + strand, within ycgEat 1212.993 kb on + strand, within ycgEat 1213.017 kb on - strand, within ycgEat 1213.051 kb on - strand, within ycgEat 1213.051 kb on - strand, within ycgEat 1213.160 kb on - strand, within ycgEat 1213.171 kb on + strand, within ycgEat 1213.247 kb on + strandat 1213.265 kb on - strandat 1213.265 kb on - strandat 1213.277 kb on - strandat 1213.301 kb on + strandat 1213.314 kb on + strandat 1213.314 kb on + strandat 1213.314 kb on + strandat 1213.314 kb on + strandat 1213.434 kb on - strandat 1213.517 kb on - strandat 1213.581 kb on + strandat 1213.592 kb on + strandat 1213.592 kb on + strandat 1213.594 kb on + strandat 1213.600 kb on - strandat 1213.602 kb on - strandat 1213.612 kb on - strand, within ycgFat 1213.612 kb on - strand, within ycgFat 1213.620 kb on - strand, within ycgFat 1213.758 kb on - strand, within ycgFat 1213.759 kb on - strand, within ycgFat 1213.789 kb on + strand, within ycgFat 1213.789 kb on + strand, within ycgFat 1213.797 kb on - strand, within ycgFat 1213.799 kb on + strand, within ycgFat 1213.799 kb on - strand, within ycgFat 1213.846 kb on - strand, within ycgFat 1213.858 kb on - strand, within ycgFat 1213.902 kb on + strand, within ycgFat 1213.910 kb on - strand, within ycgFat 1213.960 kb on - strand, within ycgFat 1213.993 kb on - strand, within ycgFat 1213.993 kb on - strand, within ycgFat 1214.007 kb on + strand, within ycgFat 1214.067 kb on + strand, within ycgFat 1214.067 kb on + strand, within ycgFat 1214.151 kb on - strand, within ycgFat 1214.190 kb on + strand, within ycgFat 1214.190 kb on + strand, within ycgFat 1214.194 kb on - strand, within ycgFat 1214.203 kb on + strand, within ycgFat 1214.203 kb on + strand, within ycgFat 1214.203 kb on + strand, within ycgFat 1214.257 kb on + strand, within ycgF

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml
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1,212,255 - ycgX b1161 0.81 +0.8
1,212,292 - +1.9
1,212,292 - +0.9
1,212,327 - -1.0
1,212,328 - +1.8
1,212,345 - -0.6
1,212,371 - +2.0
1,212,371 - +0.1
1,212,376 - +1.1
1,212,376 - -0.2
1,212,425 - -0.6
1,212,427 + +0.4
1,212,435 - -0.2
1,212,435 - +1.1
1,212,472 + -1.2
1,212,477 + -1.6
1,212,477 + -0.6
1,212,483 + -1.8
1,212,483 + -0.6
1,212,579 - -0.7
1,212,579 - +0.9
1,212,596 - +0.5
1,212,618 + +0.3
1,212,689 - ycgE b1162 0.19 +0.3
1,212,689 - ycgE b1162 0.19 +1.9
1,212,803 + ycgE b1162 0.34 +1.7
1,212,864 - ycgE b1162 0.43 +1.2
1,212,892 - ycgE b1162 0.47 -0.0
1,212,894 + ycgE b1162 0.47 +0.3
1,212,951 - ycgE b1162 0.55 +0.7
1,212,954 + ycgE b1162 0.55 +0.3
1,212,993 + ycgE b1162 0.60 -1.2
1,213,017 - ycgE b1162 0.64 +0.7
1,213,051 - ycgE b1162 0.68 +2.7
1,213,051 - ycgE b1162 0.68 +0.7
1,213,160 - ycgE b1162 0.83 +0.5
1,213,171 + ycgE b1162 0.85 +0.5
1,213,247 + +2.0
1,213,265 - -0.7
1,213,265 - -0.7
1,213,277 - +0.3
1,213,301 + +2.0
1,213,314 + -0.1
1,213,314 + -1.0
1,213,314 + +0.4
1,213,314 + +0.1
1,213,434 - -1.0
1,213,517 - +0.0
1,213,581 + +0.3
1,213,592 + +0.3
1,213,592 + +0.3
1,213,594 + -0.7
1,213,600 - +0.9
1,213,602 - +2.9
1,213,612 - ycgF b1163 0.10 +1.9
1,213,612 - ycgF b1163 0.10 +0.3
1,213,620 - ycgF b1163 0.11 -1.7
1,213,758 - ycgF b1163 0.22 -0.7
1,213,759 - ycgF b1163 0.22 +0.6
1,213,789 + ycgF b1163 0.25 +1.7
1,213,789 + ycgF b1163 0.25 -0.7
1,213,797 - ycgF b1163 0.26 +2.3
1,213,799 + ycgF b1163 0.26 +0.3
1,213,799 - ycgF b1163 0.26 -0.7
1,213,846 - ycgF b1163 0.30 -0.7
1,213,858 - ycgF b1163 0.31 -0.7
1,213,902 + ycgF b1163 0.34 +2.9
1,213,910 - ycgF b1163 0.35 +1.3
1,213,960 - ycgF b1163 0.39 +0.3
1,213,993 - ycgF b1163 0.42 -0.7
1,213,993 - ycgF b1163 0.42 -1.7
1,214,007 + ycgF b1163 0.43 -0.7
1,214,067 + ycgF b1163 0.48 -2.7
1,214,067 + ycgF b1163 0.48 +0.9
1,214,151 - ycgF b1163 0.55 -0.7
1,214,190 + ycgF b1163 0.58 +0.8
1,214,190 + ycgF b1163 0.58 -0.7
1,214,194 - ycgF b1163 0.58 +0.3
1,214,203 + ycgF b1163 0.59 +1.3
1,214,203 + ycgF b1163 0.59 +0.3
1,214,203 + ycgF b1163 0.59 -0.4
1,214,257 + ycgF b1163 0.64 +0.9

Or see this region's nucleotide sequence