Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybbO and tesA overlap by 11 nucleotides tesA and ybbA overlap by 33 nucleotides ybbA and ybbP overlap by 4 nucleotides
b0493: ybbO - short chain dehydrogenase (NCBI), at 517,564 to 518,373
ybbO
b0494: tesA - multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1 (NCBI), at 518,363 to 518,989
tesA
b0495: ybbA - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 518,957 to 519,643
ybbA
b0496: ybbP - predicted inner membrane protein (NCBI), at 519,640 to 522,054
ybbP
Position (kb) 518
519
520 Strain fitness (log2 ratio) -1
0
1 at 517.957 kb on + strand, within ybbO at 518.037 kb on + strand, within ybbO at 518.051 kb on - strand, within ybbO at 518.123 kb on + strand, within ybbO at 518.145 kb on - strand, within ybbO at 518.145 kb on - strand, within ybbO at 518.199 kb on - strand, within ybbO at 518.377 kb on - strand at 518.475 kb on + strand, within tesA at 518.483 kb on - strand, within tesA at 518.570 kb on + strand, within tesA at 518.768 kb on + strand, within tesA at 518.839 kb on - strand, within tesA at 518.839 kb on - strand, within tesA at 518.839 kb on - strand, within tesA at 519.006 kb on + strand at 519.102 kb on + strand, within ybbA at 519.181 kb on - strand, within ybbA at 519.306 kb on - strand, within ybbA at 519.306 kb on - strand, within ybbA at 519.353 kb on + strand, within ybbA at 519.353 kb on + strand at 519.354 kb on + strand, within ybbA at 519.407 kb on + strand, within ybbA at 519.407 kb on + strand, within ybbA at 519.534 kb on + strand, within ybbA at 519.707 kb on + strand at 519.770 kb on - strand at 519.770 kb on - strand at 519.908 kb on - strand, within ybbP at 519.913 kb on - strand, within ybbP at 519.981 kb on - strand, within ybbP at 520.140 kb on + strand, within ybbP at 520.269 kb on - strand, within ybbP at 520.269 kb on - strand, within ybbP at 520.316 kb on - strand, within ybbP
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 517,957 + ybbO b0493 0.49 +0.6 518,037 + ybbO b0493 0.58 +1.0 518,051 - ybbO b0493 0.60 +0.8 518,123 + ybbO b0493 0.69 -0.8 518,145 - ybbO b0493 0.72 -1.0 518,145 - ybbO b0493 0.72 -1.0 518,199 - ybbO b0493 0.78 -0.9 518,377 - -0.8 518,475 + tesA b0494 0.18 +0.6 518,483 - tesA b0494 0.19 +0.3 518,570 + tesA b0494 0.33 +1.3 518,768 + tesA b0494 0.65 +1.0 518,839 - tesA b0494 0.76 +0.3 518,839 - tesA b0494 0.76 +0.6 518,839 - tesA b0494 0.76 +0.3 519,006 + +0.5 519,102 + ybbA b0495 0.21 +1.1 519,181 - ybbA b0495 0.33 +0.2 519,306 - ybbA b0495 0.51 -0.7 519,306 - ybbA b0495 0.51 +0.1 519,353 + ybbA b0495 0.58 -0.3 519,353 + -1.6 519,354 + ybbA b0495 0.58 +0.3 519,407 + ybbA b0495 0.66 +0.2 519,407 + ybbA b0495 0.66 +1.5 519,534 + ybbA b0495 0.84 +0.2 519,707 + +0.1 519,770 - +1.0 519,770 - +0.4 519,908 - ybbP b0496 0.11 -0.2 519,913 - ybbP b0496 0.11 -0.1 519,981 - ybbP b0496 0.14 +1.0 520,140 + ybbP b0496 0.21 +0.4 520,269 - ybbP b0496 0.26 +1.0 520,269 - ybbP b0496 0.26 -1.1 520,316 - ybbP b0496 0.28 -1.1
Or see this region's nucleotide sequence