Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafK and yafQ are separated by 155 nucleotides yafQ and dinJ are separated by 2 nucleotides dinJ and yafL are separated by 209 nucleotides
b0224: yafK - hypothetical protein (NCBI), at 245,065 to 245,805
yafK
b0225: yafQ - predicted toxin of the YafQ-DinJ toxin-antitoxin system (NCBI), at 245,961 to 246,239
yafQ
b0226: dinJ - predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI), at 246,242 to 246,502
dinJ
b0227: yafL - predicted lipoprotein and C40 family peptidase (NCBI), at 246,712 to 247,461
yafL
Position (kb) 246
247 Strain fitness (log2 ratio) -2
-1
0
1
2 at 245.278 kb on - strand, within yafK at 245.278 kb on - strand, within yafK at 245.285 kb on + strand, within yafK at 245.296 kb on - strand, within yafK at 245.315 kb on - strand, within yafK at 245.315 kb on - strand, within yafK at 245.355 kb on - strand, within yafK at 245.357 kb on - strand, within yafK at 245.388 kb on - strand, within yafK at 245.388 kb on - strand, within yafK at 245.407 kb on + strand, within yafK at 245.431 kb on - strand, within yafK at 245.448 kb on - strand, within yafK at 245.480 kb on - strand, within yafK at 245.524 kb on - strand, within yafK at 245.524 kb on - strand, within yafK at 245.526 kb on + strand, within yafK at 245.611 kb on + strand, within yafK at 245.611 kb on + strand, within yafK at 245.671 kb on + strand, within yafK at 245.679 kb on - strand, within yafK at 245.704 kb on - strand, within yafK at 245.704 kb on - strand at 245.706 kb on - strand, within yafK at 245.706 kb on - strand, within yafK at 245.706 kb on - strand, within yafK at 245.735 kb on - strand at 245.743 kb on - strand at 245.743 kb on - strand at 245.837 kb on + strand at 245.928 kb on - strand at 245.928 kb on - strand at 245.964 kb on + strand at 245.964 kb on + strand at 245.971 kb on - strand at 246.048 kb on + strand, within yafQ at 246.048 kb on + strand, within yafQ at 246.048 kb on + strand, within yafQ at 246.086 kb on - strand, within yafQ at 246.086 kb on - strand, within yafQ at 246.086 kb on - strand, within yafQ at 246.086 kb on - strand, within yafQ at 246.097 kb on - strand, within yafQ at 246.294 kb on - strand, within dinJ at 246.295 kb on - strand, within dinJ at 246.304 kb on - strand, within dinJ at 246.330 kb on - strand, within dinJ at 246.338 kb on - strand, within dinJ at 246.338 kb on - strand, within dinJ at 246.411 kb on + strand, within dinJ at 246.411 kb on + strand, within dinJ at 246.742 kb on - strand at 246.821 kb on - strand, within yafL at 246.854 kb on + strand, within yafL at 246.859 kb on + strand, within yafL at 247.129 kb on - strand, within yafL at 247.129 kb on - strand, within yafL at 247.227 kb on - strand, within yafL at 247.227 kb on - strand, within yafL at 247.288 kb on + strand, within yafL at 247.312 kb on - strand, within yafL at 247.409 kb on - strand at 247.493 kb on + strand at 247.502 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 245,278 - yafK b0224 0.29 +1.3 245,278 - yafK b0224 0.29 -1.6 245,285 + yafK b0224 0.30 +1.8 245,296 - yafK b0224 0.31 -0.3 245,315 - yafK b0224 0.34 -1.0 245,315 - yafK b0224 0.34 +0.7 245,355 - yafK b0224 0.39 -1.0 245,357 - yafK b0224 0.39 +0.9 245,388 - yafK b0224 0.44 -1.9 245,388 - yafK b0224 0.44 +0.2 245,407 + yafK b0224 0.46 -0.4 245,431 - yafK b0224 0.49 -0.6 245,448 - yafK b0224 0.52 -0.2 245,480 - yafK b0224 0.56 +0.2 245,524 - yafK b0224 0.62 +0.0 245,524 - yafK b0224 0.62 +0.1 245,526 + yafK b0224 0.62 -0.8 245,611 + yafK b0224 0.74 +0.1 245,611 + yafK b0224 0.74 +0.3 245,671 + yafK b0224 0.82 +0.2 245,679 - yafK b0224 0.83 -1.1 245,704 - yafK b0224 0.86 -1.5 245,704 - -1.8 245,706 - yafK b0224 0.87 -2.6 245,706 - yafK b0224 0.87 +0.7 245,706 - yafK b0224 0.87 -1.2 245,735 - +1.5 245,743 - +1.2 245,743 - +1.1 245,837 + +0.7 245,928 - -0.4 245,928 - +0.1 245,964 + -0.2 245,964 + +0.3 245,971 - +0.6 246,048 + yafQ b0225 0.31 -1.5 246,048 + yafQ b0225 0.31 +0.8 246,048 + yafQ b0225 0.31 +1.4 246,086 - yafQ b0225 0.45 +0.8 246,086 - yafQ b0225 0.45 +0.5 246,086 - yafQ b0225 0.45 +0.5 246,086 - yafQ b0225 0.45 +2.1 246,097 - yafQ b0225 0.49 -1.2 246,294 - dinJ b0226 0.20 -1.3 246,295 - dinJ b0226 0.20 +1.6 246,304 - dinJ b0226 0.24 +0.3 246,330 - dinJ b0226 0.34 +1.9 246,338 - dinJ b0226 0.37 -0.5 246,338 - dinJ b0226 0.37 +1.1 246,411 + dinJ b0226 0.65 -1.1 246,411 + dinJ b0226 0.65 +1.2 246,742 - -0.3 246,821 - yafL b0227 0.15 +0.2 246,854 + yafL b0227 0.19 -0.2 246,859 + yafL b0227 0.20 +0.5 247,129 - yafL b0227 0.56 +1.3 247,129 - yafL b0227 0.56 +0.8 247,227 - yafL b0227 0.69 -1.1 247,227 - yafL b0227 0.69 +1.7 247,288 + yafL b0227 0.77 +0.2 247,312 - yafL b0227 0.80 +0.6 247,409 - +0.2 247,493 + +0.8 247,502 - +0.1
Or see this region's nucleotide sequence