Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rstB and tus are separated by 75 nucleotides tus and fumC overlap by 4 nucleotides
b1609: rstB - sensory histidine kinase in two-component regulatory system with RstA (NCBI), at 1,680,906 to 1,682,207
rstB
b1610: tus - DNA replication terminus site-binding protein (NCBI), at 1,682,283 to 1,683,212
tus
b1611: fumC - fumarate hydratase (NCBI), at 1,683,209 to 1,684,612
fumC
Position (kb)
1682
1683
1684 Strain fitness (log2 ratio)
-1
0
1
2 at 1681.555 kb on + strand, within rstB at 1681.555 kb on + strand, within rstB at 1681.779 kb on - strand, within rstB at 1681.779 kb on - strand, within rstB at 1681.827 kb on + strand, within rstB at 1682.011 kb on + strand, within rstB at 1682.068 kb on + strand, within rstB at 1682.068 kb on + strand, within rstB at 1682.090 kb on + strand at 1682.098 kb on - strand at 1682.100 kb on - strand at 1682.102 kb on + strand at 1682.102 kb on + strand at 1682.223 kb on + strand at 1682.223 kb on + strand at 1682.306 kb on - strand at 1682.451 kb on - strand, within tus at 1682.567 kb on - strand, within tus at 1682.620 kb on + strand, within tus at 1682.620 kb on + strand, within tus at 1682.820 kb on + strand, within tus at 1682.838 kb on + strand, within tus at 1682.878 kb on + strand, within tus at 1682.886 kb on - strand, within tus at 1682.941 kb on - strand, within tus at 1682.941 kb on - strand, within tus at 1683.046 kb on + strand, within tus at 1683.046 kb on + strand, within tus at 1683.207 kb on + strand at 1683.241 kb on + strand at 1683.295 kb on + strand at 1683.295 kb on + strand at 1683.439 kb on + strand, within fumC at 1683.595 kb on + strand, within fumC at 1683.612 kb on + strand, within fumC at 1683.612 kb on + strand, within fumC at 1683.665 kb on - strand, within fumC at 1683.665 kb on - strand, within fumC at 1683.709 kb on + strand, within fumC at 1683.709 kb on + strand, within fumC at 1683.859 kb on - strand, within fumC at 1683.859 kb on - strand, within fumC at 1683.981 kb on - strand, within fumC at 1684.016 kb on - strand, within fumC at 1684.016 kb on - strand, within fumC
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 1,681,555 + rstB b1609 0.50 +0.6 1,681,555 + rstB b1609 0.50 +0.6 1,681,779 - rstB b1609 0.67 -1.2 1,681,779 - rstB b1609 0.67 +0.3 1,681,827 + rstB b1609 0.71 +0.2 1,682,011 + rstB b1609 0.85 +0.0 1,682,068 + rstB b1609 0.89 +0.0 1,682,068 + rstB b1609 0.89 +1.0 1,682,090 + +0.3 1,682,098 - -0.4 1,682,100 - -1.4 1,682,102 + -0.4 1,682,102 + +0.5 1,682,223 + -0.4 1,682,223 + +0.3 1,682,306 - +0.6 1,682,451 - tus b1610 0.18 +0.2 1,682,567 - tus b1610 0.31 -0.7 1,682,620 + tus b1610 0.36 +0.8 1,682,620 + tus b1610 0.36 -0.5 1,682,820 + tus b1610 0.58 +0.2 1,682,838 + tus b1610 0.60 +0.6 1,682,878 + tus b1610 0.64 +0.1 1,682,886 - tus b1610 0.65 +0.2 1,682,941 - tus b1610 0.71 -0.8 1,682,941 - tus b1610 0.71 +1.0 1,683,046 + tus b1610 0.82 +0.9 1,683,046 + tus b1610 0.82 +0.5 1,683,207 + +0.5 1,683,241 + +2.0 1,683,295 + -1.0 1,683,295 + +0.5 1,683,439 + fumC b1611 0.16 +0.1 1,683,595 + fumC b1611 0.27 -0.4 1,683,612 + fumC b1611 0.29 -0.5 1,683,612 + fumC b1611 0.29 +0.5 1,683,665 - fumC b1611 0.32 -1.0 1,683,665 - fumC b1611 0.32 -0.0 1,683,709 + fumC b1611 0.36 +0.2 1,683,709 + fumC b1611 0.36 -0.4 1,683,859 - fumC b1611 0.46 +0.6 1,683,859 - fumC b1611 0.46 +0.2 1,683,981 - fumC b1611 0.55 +0.6 1,684,016 - fumC b1611 0.57 -0.3 1,684,016 - fumC b1611 0.57 -0.8
Or see this region's nucleotide sequence