Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hlyE and umuD are separated by 372 nucleotides umuD and umuC overlap by 1 nucleotides umuC and dsbB are separated by 45 nucleotides dsbB and nhaB are separated by 145 nucleotides
b1182: hlyE - hemolysin E (VIMSS), at 1,228,706 to 1,229,617
hlyE
b1183: umuD - DNA polymerase V, subunit D (NCBI), at 1,229,990 to 1,230,409
umuD
b1184: umuC - DNA polymerase V subunit UmuC (NCBI), at 1,230,409 to 1,231,677
umuC
b1185: dsbB - reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS), at 1,231,723 to 1,232,253
dsbB
b1186: nhaB - NhaB sodium/proton transporter (Katherine Huang), at 1,232,399 to 1,233,940
nhaB
Position (kb)
1230
1231
1232 Strain fitness (log2 ratio)
-2
-1
0
1 at 1229.669 kb on - strand at 1229.703 kb on - strand at 1229.704 kb on - strand at 1229.718 kb on - strand at 1229.718 kb on - strand at 1229.838 kb on - strand at 1229.840 kb on - strand at 1229.842 kb on - strand at 1229.842 kb on - strand at 1229.844 kb on - strand at 1229.851 kb on - strand at 1229.851 kb on - strand at 1229.853 kb on - strand at 1229.859 kb on - strand at 1229.859 kb on - strand at 1229.859 kb on - strand at 1229.861 kb on + strand at 1229.897 kb on - strand at 1229.897 kb on - strand at 1230.140 kb on + strand, within umuD at 1230.307 kb on + strand, within umuD at 1230.446 kb on + strand at 1230.893 kb on + strand, within umuC at 1230.956 kb on + strand, within umuC at 1231.013 kb on - strand, within umuC at 1231.029 kb on + strand, within umuC at 1231.038 kb on - strand, within umuC at 1231.109 kb on + strand, within umuC at 1231.109 kb on + strand, within umuC at 1231.208 kb on + strand at 1231.208 kb on + strand, within umuC at 1231.270 kb on + strand, within umuC at 1231.270 kb on + strand, within umuC at 1231.320 kb on + strand, within umuC at 1231.359 kb on + strand, within umuC at 1231.359 kb on + strand, within umuC at 1231.382 kb on - strand, within umuC at 1231.457 kb on + strand, within umuC at 1231.519 kb on - strand, within umuC at 1231.550 kb on + strand, within umuC at 1231.551 kb on - strand at 1231.553 kb on - strand at 1231.573 kb on - strand at 1231.597 kb on + strand at 1231.625 kb on + strand at 1231.627 kb on - strand at 1231.643 kb on - strand at 1231.645 kb on - strand at 1231.660 kb on - strand at 1231.961 kb on + strand, within dsbB at 1231.961 kb on + strand, within dsbB at 1231.979 kb on - strand, within dsbB at 1232.074 kb on + strand, within dsbB at 1232.074 kb on + strand, within dsbB at 1232.077 kb on - strand, within dsbB at 1232.272 kb on + strand at 1232.272 kb on + strand at 1232.504 kb on - strand at 1232.509 kb on - strand at 1232.525 kb on + strand at 1232.569 kb on - strand, within nhaB
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 1,229,669 - +0.7 1,229,703 - +0.4 1,229,704 - +1.0 1,229,718 - +0.7 1,229,718 - +0.7 1,229,838 - -0.5 1,229,840 - +0.8 1,229,842 - +0.1 1,229,842 - +0.4 1,229,844 - -0.3 1,229,851 - +0.2 1,229,851 - +0.2 1,229,853 - -0.1 1,229,859 - +0.6 1,229,859 - +0.7 1,229,859 - +1.2 1,229,861 + +0.7 1,229,897 - +0.2 1,229,897 - -1.9 1,230,140 + umuD b1183 0.36 -1.2 1,230,307 + umuD b1183 0.75 -0.9 1,230,446 + -1.6 1,230,893 + umuC b1184 0.38 -2.2 1,230,956 + umuC b1184 0.43 -1.0 1,231,013 - umuC b1184 0.48 -0.9 1,231,029 + umuC b1184 0.49 -0.9 1,231,038 - umuC b1184 0.50 -1.1 1,231,109 + umuC b1184 0.55 -1.3 1,231,109 + umuC b1184 0.55 -1.2 1,231,208 + -0.9 1,231,208 + umuC b1184 0.63 -2.1 1,231,270 + umuC b1184 0.68 -1.1 1,231,270 + umuC b1184 0.68 -1.2 1,231,320 + umuC b1184 0.72 -1.6 1,231,359 + umuC b1184 0.75 -1.2 1,231,359 + umuC b1184 0.75 -1.3 1,231,382 - umuC b1184 0.77 -2.6 1,231,457 + umuC b1184 0.83 -0.5 1,231,519 - umuC b1184 0.87 -0.7 1,231,550 + umuC b1184 0.90 -0.6 1,231,551 - -2.6 1,231,553 - -0.6 1,231,573 - -0.6 1,231,597 + -1.9 1,231,625 + -0.9 1,231,627 - -0.9 1,231,643 - -1.9 1,231,645 - -0.7 1,231,660 - +0.7 1,231,961 + dsbB b1185 0.45 -1.5 1,231,961 + dsbB b1185 0.45 -0.9 1,231,979 - dsbB b1185 0.48 -1.1 1,232,074 + dsbB b1185 0.66 -1.3 1,232,074 + dsbB b1185 0.66 -0.3 1,232,077 - dsbB b1185 0.67 +0.7 1,232,272 + -0.0 1,232,272 + -0.4 1,232,504 - +0.4 1,232,509 - -1.1 1,232,525 + +0.3 1,232,569 - nhaB b1186 0.11 -0.0
Or see this region's nucleotide sequence