Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bioF and bioC overlap by 14 nucleotides bioC and bioD overlap by 8 nucleotides bioD and uvrB are separated by 578 nucleotides
b0776: bioF - 8-amino-7-oxononanoate synthase (NCBI), at 809,604 to 810,758
bioF
b0777: bioC - predicted methltransferase, enzyme of biotin synthesis (NCBI), at 810,745 to 811,500
bioC
b0778: bioD - dethiobiotin synthetase (NCBI), at 811,493 to 812,170
bioD
b0779: uvrB - excinuclease ABC subunit B (NCBI), at 812,749 to 814,770
uvrB
Position (kb) 811
812
813 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 810.673 kb on + strand at 810.673 kb on - strand at 810.729 kb on + strand at 810.791 kb on + strand at 810.833 kb on + strand, within bioC at 810.971 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.326 kb on + strand, within bioC at 811.326 kb on + strand, within bioC at 811.326 kb on + strand, within bioC at 811.366 kb on - strand, within bioC at 811.423 kb on + strand, within bioC at 811.423 kb on + strand, within bioC at 811.431 kb on - strand at 811.446 kb on - strand at 811.446 kb on - strand at 811.464 kb on - strand at 811.559 kb on + strand at 811.672 kb on + strand, within bioD at 811.720 kb on - strand, within bioD at 811.757 kb on + strand, within bioD at 812.000 kb on + strand, within bioD at 812.005 kb on - strand, within bioD at 812.005 kb on - strand, within bioD at 812.051 kb on + strand, within bioD at 812.051 kb on + strand, within bioD at 812.061 kb on - strand, within bioD at 812.295 kb on - strand at 812.353 kb on - strand at 812.834 kb on + strand at 812.957 kb on + strand, within uvrB at 812.967 kb on - strand, within uvrB at 813.039 kb on + strand, within uvrB at 813.050 kb on + strand, within uvrB at 813.055 kb on - strand, within uvrB at 813.106 kb on - strand, within uvrB at 813.106 kb on - strand, within uvrB
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 810,673 + +1.2 810,673 - +0.2 810,729 + +0.2 810,791 + +0.7 810,833 + bioC b0777 0.12 +0.4 810,971 + bioC b0777 0.30 +0.4 811,122 + bioC b0777 0.50 +0.6 811,122 + bioC b0777 0.50 +1.1 811,122 + bioC b0777 0.50 +0.0 811,122 + bioC b0777 0.50 +1.1 811,326 + bioC b0777 0.77 -0.3 811,326 + bioC b0777 0.77 -0.2 811,326 + bioC b0777 0.77 -0.1 811,366 - bioC b0777 0.82 +0.5 811,423 + bioC b0777 0.90 +0.6 811,423 + bioC b0777 0.90 +0.6 811,431 - -0.0 811,446 - +0.4 811,446 - -0.2 811,464 - -1.0 811,559 + -1.3 811,672 + bioD b0778 0.26 -0.6 811,720 - bioD b0778 0.33 -0.1 811,757 + bioD b0778 0.39 -0.6 812,000 + bioD b0778 0.75 +0.6 812,005 - bioD b0778 0.76 -1.5 812,005 - bioD b0778 0.76 -0.6 812,051 + bioD b0778 0.82 -0.0 812,051 + bioD b0778 0.82 -0.5 812,061 - bioD b0778 0.84 -0.2 812,295 - -0.1 812,353 - -0.0 812,834 + -3.0 812,957 + uvrB b0779 0.10 -1.5 812,967 - uvrB b0779 0.11 -2.5 813,039 + uvrB b0779 0.14 -3.6 813,050 + uvrB b0779 0.15 -3.0 813,055 - uvrB b0779 0.15 -3.9 813,106 - uvrB b0779 0.18 -2.6 813,106 - uvrB b0779 0.18 -1.2
Or see this region's nucleotide sequence