Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrD and yigE are separated by 146 nucleotides yigE and corA are separated by 369 nucleotides
b3813: uvrD - DNA-dependent ATPase I and helicase II (NCBI), at 3,996,006 to 3,998,168
uvrD
b4482: yigE - hypothetical protein (NCBI), at 3,998,315 to 3,999,079
yigE
b3816: corA - magnesium/nickel/cobalt transporter (NCBI), at 3,999,449 to 4,000,399
corA
Position (kb)
3998
3999
4000 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3997.327 kb on + strand, within uvrD at 3997.410 kb on + strand, within uvrD at 3997.418 kb on - strand, within uvrD at 3997.439 kb on + strand, within uvrD at 3997.439 kb on + strand, within uvrD at 3997.443 kb on - strand, within uvrD at 3997.465 kb on + strand, within uvrD at 3997.465 kb on + strand, within uvrD at 3997.472 kb on + strand, within uvrD at 3997.482 kb on + strand, within uvrD at 3997.484 kb on - strand, within uvrD at 3997.484 kb on - strand, within uvrD at 3997.501 kb on - strand, within uvrD at 3997.501 kb on - strand, within uvrD at 3997.544 kb on - strand, within uvrD at 3997.544 kb on - strand, within uvrD at 3997.633 kb on - strand, within uvrD at 3997.670 kb on + strand, within uvrD at 3997.670 kb on + strand, within uvrD at 3997.670 kb on + strand, within uvrD at 3997.685 kb on - strand, within uvrD at 3997.685 kb on - strand, within uvrD at 3997.685 kb on - strand, within uvrD at 3997.685 kb on - strand, within uvrD at 3997.695 kb on + strand, within uvrD at 3997.731 kb on + strand, within uvrD at 3997.731 kb on + strand, within uvrD at 3997.771 kb on - strand, within uvrD at 3997.771 kb on - strand, within uvrD at 3997.809 kb on + strand, within uvrD at 3997.809 kb on + strand, within uvrD at 3997.817 kb on - strand, within uvrD at 3997.867 kb on + strand, within uvrD at 3997.904 kb on - strand, within uvrD at 3997.922 kb on - strand, within uvrD at 3997.952 kb on + strand at 3997.959 kb on - strand at 3997.969 kb on + strand at 3997.976 kb on + strand at 3997.976 kb on + strand at 3997.977 kb on - strand at 3998.057 kb on - strand at 3998.087 kb on + strand at 3998.087 kb on + strand at 3998.163 kb on + strand at 3998.171 kb on - strand at 3998.171 kb on - strand at 3998.171 kb on - strand at 3998.257 kb on - strand at 3998.343 kb on - strand at 3998.382 kb on + strand at 3998.420 kb on - strand, within yigE at 3998.529 kb on - strand, within yigE at 3998.572 kb on + strand, within yigE at 3998.654 kb on - strand, within yigE at 3998.658 kb on - strand, within yigE at 3998.717 kb on + strand, within yigE at 3998.776 kb on - strand, within yigE at 3998.824 kb on + strand, within yigE at 3998.866 kb on - strand, within yigE at 3998.912 kb on - strand, within yigE at 3998.912 kb on - strand, within yigE at 3998.917 kb on + strand, within yigE at 3998.939 kb on + strand, within yigE at 3998.941 kb on + strand, within yigE at 3998.942 kb on - strand, within yigE at 3998.947 kb on - strand, within yigE at 3998.947 kb on - strand, within yigE at 3999.078 kb on + strand at 3999.084 kb on - strand at 3999.084 kb on - strand at 3999.127 kb on - strand at 3999.127 kb on - strand at 3999.127 kb on - strand at 3999.162 kb on + strand at 3999.167 kb on + strand at 3999.209 kb on - strand at 3999.222 kb on + strand at 3999.222 kb on + strand at 3999.230 kb on - strand at 3999.230 kb on - strand at 3999.230 kb on - strand at 3999.238 kb on + strand at 3999.254 kb on + strand at 3999.257 kb on + strand at 3999.257 kb on + strand at 3999.279 kb on + strand at 3999.284 kb on + strand at 3999.284 kb on + strand at 3999.299 kb on - strand at 3999.300 kb on - strand at 3999.333 kb on - strand at 3999.333 kb on - strand at 3999.361 kb on + strand at 3999.399 kb on - strand at 3999.407 kb on + strand at 3999.407 kb on + strand at 3999.407 kb on + strand at 3999.548 kb on - strand, within corA at 3999.582 kb on + strand at 3999.582 kb on + strand, within corA at 3999.584 kb on + strand, within corA at 3999.588 kb on + strand, within corA at 3999.664 kb on + strand, within corA at 3999.664 kb on + strand, within corA at 3999.664 kb on + strand, within corA at 3999.665 kb on + strand, within corA at 3999.665 kb on + strand, within corA at 3999.718 kb on - strand, within corA at 3999.718 kb on - strand, within corA at 3999.723 kb on - strand, within corA at 3999.743 kb on - strand, within corA at 3999.751 kb on + strand, within corA at 3999.758 kb on - strand, within corA at 3999.758 kb on - strand, within corA at 3999.765 kb on + strand, within corA at 3999.768 kb on - strand, within corA at 3999.768 kb on - strand, within corA at 3999.797 kb on - strand, within corA at 3999.797 kb on - strand, within corA at 3999.804 kb on + strand, within corA at 3999.826 kb on + strand, within corA at 3999.877 kb on + strand, within corA at 3999.877 kb on - strand, within corA at 3999.898 kb on + strand, within corA at 3999.898 kb on + strand, within corA at 3999.898 kb on + strand, within corA at 3999.899 kb on + strand, within corA at 3999.958 kb on + strand, within corA at 3999.970 kb on + strand, within corA at 3999.970 kb on + strand, within corA at 3999.978 kb on - strand, within corA at 3999.978 kb on - strand, within corA at 4000.008 kb on + strand, within corA at 4000.019 kb on + strand, within corA at 4000.040 kb on + strand, within corA at 4000.040 kb on + strand, within corA at 4000.077 kb on - strand, within corA at 4000.077 kb on - strand, within corA
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 3,997,327 + uvrD b3813 0.61 +0.6 3,997,410 + uvrD b3813 0.65 +0.6 3,997,418 - uvrD b3813 0.65 -1.5 3,997,439 + uvrD b3813 0.66 -0.3 3,997,439 + uvrD b3813 0.66 +2.5 3,997,443 - uvrD b3813 0.66 -0.8 3,997,465 + uvrD b3813 0.67 +1.3 3,997,465 + uvrD b3813 0.67 +0.0 3,997,472 + uvrD b3813 0.68 +0.2 3,997,482 + uvrD b3813 0.68 -0.1 3,997,484 - uvrD b3813 0.68 -0.9 3,997,484 - uvrD b3813 0.68 -0.4 3,997,501 - uvrD b3813 0.69 -0.7 3,997,501 - uvrD b3813 0.69 -0.6 3,997,544 - uvrD b3813 0.71 -0.7 3,997,544 - uvrD b3813 0.71 -0.6 3,997,633 - uvrD b3813 0.75 -0.3 3,997,670 + uvrD b3813 0.77 +0.8 3,997,670 + uvrD b3813 0.77 +0.5 3,997,670 + uvrD b3813 0.77 -0.0 3,997,685 - uvrD b3813 0.78 -0.5 3,997,685 - uvrD b3813 0.78 -1.6 3,997,685 - uvrD b3813 0.78 +0.1 3,997,685 - uvrD b3813 0.78 -0.3 3,997,695 + uvrD b3813 0.78 +1.0 3,997,731 + uvrD b3813 0.80 +0.3 3,997,731 + uvrD b3813 0.80 +0.1 3,997,771 - uvrD b3813 0.82 -0.4 3,997,771 - uvrD b3813 0.82 -1.2 3,997,809 + uvrD b3813 0.83 +0.5 3,997,809 + uvrD b3813 0.83 +0.3 3,997,817 - uvrD b3813 0.84 -1.3 3,997,867 + uvrD b3813 0.86 +0.2 3,997,904 - uvrD b3813 0.88 -1.1 3,997,922 - uvrD b3813 0.89 -1.8 3,997,952 + -0.1 3,997,959 - -0.4 3,997,969 + +1.0 3,997,976 + +0.8 3,997,976 + +0.2 3,997,977 - -1.1 3,998,057 - -0.7 3,998,087 + +0.8 3,998,087 + +0.3 3,998,163 + +0.5 3,998,171 - -0.5 3,998,171 - +0.2 3,998,171 - -0.6 3,998,257 - -0.7 3,998,343 - -1.1 3,998,382 + +0.4 3,998,420 - yigE b4482 0.14 +0.1 3,998,529 - yigE b4482 0.28 -0.8 3,998,572 + yigE b4482 0.34 +0.4 3,998,654 - yigE b4482 0.44 -0.6 3,998,658 - yigE b4482 0.45 -1.1 3,998,717 + yigE b4482 0.53 +0.4 3,998,776 - yigE b4482 0.60 -2.1 3,998,824 + yigE b4482 0.67 +0.2 3,998,866 - yigE b4482 0.72 -3.0 3,998,912 - yigE b4482 0.78 -1.0 3,998,912 - yigE b4482 0.78 -1.0 3,998,917 + yigE b4482 0.79 -3.3 3,998,939 + yigE b4482 0.82 -0.3 3,998,941 + yigE b4482 0.82 -4.2 3,998,942 - yigE b4482 0.82 -1.1 3,998,947 - yigE b4482 0.83 -1.2 3,998,947 - yigE b4482 0.83 -1.4 3,999,078 + -2.4 3,999,084 - -0.5 3,999,084 - -0.4 3,999,127 - -3.3 3,999,127 - -2.5 3,999,127 - -0.9 3,999,162 + -1.3 3,999,167 + +0.6 3,999,209 - +1.3 3,999,222 + +1.4 3,999,222 + +1.3 3,999,230 - +3.2 3,999,230 - +0.8 3,999,230 - +0.7 3,999,238 + +0.8 3,999,254 + +1.3 3,999,257 + +1.2 3,999,257 + +1.3 3,999,279 + +1.8 3,999,284 + +1.0 3,999,284 + +1.8 3,999,299 - +0.8 3,999,300 - +1.7 3,999,333 - +1.2 3,999,333 - +1.1 3,999,361 + +0.5 3,999,399 - +1.2 3,999,407 + -2.4 3,999,407 + -0.2 3,999,407 + -5.1 3,999,548 - corA b3816 0.10 +0.1 3,999,582 + +1.9 3,999,582 + corA b3816 0.14 +1.6 3,999,584 + corA b3816 0.14 +1.1 3,999,588 + corA b3816 0.15 +0.8 3,999,664 + corA b3816 0.23 +1.2 3,999,664 + corA b3816 0.23 +1.2 3,999,664 + corA b3816 0.23 +2.2 3,999,665 + corA b3816 0.23 +1.2 3,999,665 + corA b3816 0.23 +1.5 3,999,718 - corA b3816 0.28 +0.9 3,999,718 - corA b3816 0.28 +0.8 3,999,723 - corA b3816 0.29 -0.9 3,999,743 - corA b3816 0.31 +0.3 3,999,751 + corA b3816 0.32 +1.0 3,999,758 - corA b3816 0.32 +1.5 3,999,758 - corA b3816 0.32 +1.0 3,999,765 + corA b3816 0.33 +1.2 3,999,768 - corA b3816 0.34 +0.9 3,999,768 - corA b3816 0.34 -0.1 3,999,797 - corA b3816 0.37 +1.3 3,999,797 - corA b3816 0.37 +1.1 3,999,804 + corA b3816 0.37 +0.5 3,999,826 + corA b3816 0.40 +1.4 3,999,877 + corA b3816 0.45 +0.5 3,999,877 - corA b3816 0.45 +0.7 3,999,898 + corA b3816 0.47 +1.1 3,999,898 + corA b3816 0.47 +1.8 3,999,898 + corA b3816 0.47 -0.1 3,999,899 + corA b3816 0.47 +0.8 3,999,958 + corA b3816 0.54 +1.7 3,999,970 + corA b3816 0.55 +2.1 3,999,970 + corA b3816 0.55 +1.1 3,999,978 - corA b3816 0.56 +0.9 3,999,978 - corA b3816 0.56 +0.0 4,000,008 + corA b3816 0.59 +1.4 4,000,019 + corA b3816 0.60 +0.8 4,000,040 + corA b3816 0.62 +1.3 4,000,040 + corA b3816 0.62 +2.3 4,000,077 - corA b3816 0.66 -0.3 4,000,077 - corA b3816 0.66 -0.2
Or see this region's nucleotide sequence