Strain Fitness in Escherichia coli BW25113 around b4501

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb2361 and yfdS overlap by 10 nucleotidesyfdS and yfdT overlap by 1 nucleotidesyfdT and ypdJ are separated by 2 nucleotidesypdJ and torI are separated by 78 nucleotidestorI and pawZ are separated by 73 nucleotidespawZ and dsdC are separated by 95 nucleotides b2361: b2361 - orf, hypothetical protein (VIMSS), at 2,473,006 to 2,473,542 b2361 b2362: yfdS - CPS-53 (KpLE1) prophage; predicted protein (NCBI), at 2,473,533 to 2,473,895 yfdS b2363: yfdT - CPS-53 (KpLE1) prophage; predicted protein (NCBI), at 2,473,895 to 2,474,200 yfdT b4545: ypdJ - no description, at 2,474,203 to 2,474,253 ypdJ b4501: torI - response regulator inhibitor for tor operon (NCBI), at 2,474,332 to 2,474,532 torI b4643: pawZ - tRNA-OTHER (RefSeq), at 2,474,606 to 2,474,620 pawZ b2364: dsdC - DNA-binding transcriptional dual regulator (NCBI), at 2,474,716 to 2,475,651 dsdC Position (kb) 2474 2475Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2473.333 kb on + strand, within b2361at 2473.333 kb on + strand, within b2361at 2473.341 kb on - strand, within b2361at 2473.351 kb on - strand, within b2361at 2473.410 kb on - strand, within b2361at 2473.445 kb on - strand, within b2361at 2473.479 kb on - strand, within b2361at 2473.607 kb on + strand, within yfdSat 2473.627 kb on - strand, within yfdSat 2473.627 kb on - strand, within yfdSat 2473.629 kb on + strand, within yfdSat 2473.649 kb on + strand, within yfdSat 2473.724 kb on + strand, within yfdSat 2473.740 kb on + strand, within yfdSat 2473.763 kb on + strand, within yfdSat 2473.770 kb on + strand, within yfdSat 2473.788 kb on - strand, within yfdSat 2473.788 kb on - strand, within yfdSat 2473.866 kb on + strandat 2473.888 kb on + strandat 2473.888 kb on + strandat 2473.950 kb on + strand, within yfdTat 2473.950 kb on + strand, within yfdTat 2473.950 kb on + strand, within yfdTat 2473.950 kb on + strand, within yfdTat 2473.970 kb on + strand, within yfdTat 2473.982 kb on + strand, within yfdTat 2474.110 kb on - strand, within yfdTat 2474.119 kb on + strand, within yfdTat 2474.127 kb on - strand, within yfdTat 2474.213 kb on - strand, within ypdJat 2474.221 kb on - strand, within ypdJat 2474.227 kb on - strand, within ypdJat 2474.316 kb on + strandat 2474.316 kb on + strandat 2474.320 kb on - strandat 2474.329 kb on + strandat 2474.343 kb on + strandat 2474.344 kb on + strandat 2474.352 kb on - strand, within torIat 2474.390 kb on - strand, within torIat 2474.390 kb on - strand, within torIat 2474.453 kb on - strand, within torIat 2474.480 kb on + strand, within torIat 2474.634 kb on + strandat 2474.668 kb on + strandat 2474.689 kb on - strandat 2474.691 kb on + strandat 2474.734 kb on - strandat 2474.734 kb on - strandat 2474.747 kb on - strandat 2474.809 kb on - strandat 2475.037 kb on - strand, within dsdCat 2475.057 kb on - strand, within dsdCat 2475.123 kb on - strand, within dsdCat 2475.188 kb on - strand, within dsdCat 2475.188 kb on - strand, within dsdCat 2475.231 kb on + strand, within dsdCat 2475.309 kb on + strand, within dsdCat 2475.312 kb on - strand, within dsdCat 2475.320 kb on + strand, within dsdCat 2475.382 kb on + strand, within dsdCat 2475.404 kb on - strand, within dsdCat 2475.477 kb on - strand, within dsdCat 2475.488 kb on + strand, within dsdC

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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2,473,333 + b2361 b2361 0.61 -0.3
2,473,333 + b2361 b2361 0.61 -0.7
2,473,341 - b2361 b2361 0.62 +1.1
2,473,351 - b2361 b2361 0.64 -0.1
2,473,410 - b2361 b2361 0.75 +0.0
2,473,445 - b2361 b2361 0.82 +0.0
2,473,479 - b2361 b2361 0.88 -0.3
2,473,607 + yfdS b2362 0.20 -0.5
2,473,627 - yfdS b2362 0.26 +0.5
2,473,627 - yfdS b2362 0.26 -0.5
2,473,629 + yfdS b2362 0.26 +0.4
2,473,649 + yfdS b2362 0.32 -0.6
2,473,724 + yfdS b2362 0.53 -0.4
2,473,740 + yfdS b2362 0.57 -0.3
2,473,763 + yfdS b2362 0.63 -1.4
2,473,770 + yfdS b2362 0.65 +0.5
2,473,788 - yfdS b2362 0.70 +1.5
2,473,788 - yfdS b2362 0.70 +1.2
2,473,866 + -1.1
2,473,888 + -1.5
2,473,888 + -0.7
2,473,950 + yfdT b2363 0.18 -1.8
2,473,950 + yfdT b2363 0.18 -1.5
2,473,950 + yfdT b2363 0.18 -0.7
2,473,950 + yfdT b2363 0.18 -2.7
2,473,970 + yfdT b2363 0.25 +0.5
2,473,982 + yfdT b2363 0.28 -1.1
2,474,110 - yfdT b2363 0.70 +0.7
2,474,119 + yfdT b2363 0.73 +0.5
2,474,127 - yfdT b2363 0.76 -0.3
2,474,213 - ypdJ b4545 0.20 +0.5
2,474,221 - ypdJ b4545 0.35 +0.5
2,474,227 - ypdJ b4545 0.47 -0.8
2,474,316 + +2.8
2,474,316 + -0.3
2,474,320 - -0.2
2,474,329 + +0.7
2,474,343 + -0.2
2,474,344 + -0.6
2,474,352 - torI b4501 0.10 +0.2
2,474,390 - torI b4501 0.29 +0.2
2,474,390 - torI b4501 0.29 +0.3
2,474,453 - torI b4501 0.60 +0.4
2,474,480 + torI b4501 0.74 +0.5
2,474,634 + -0.0
2,474,668 + -0.2
2,474,689 - -0.3
2,474,691 + +0.0
2,474,734 - -0.3
2,474,734 - +0.6
2,474,747 - +0.8
2,474,809 - +0.1
2,475,037 - dsdC b2364 0.34 -0.4
2,475,057 - dsdC b2364 0.36 +0.6
2,475,123 - dsdC b2364 0.43 -1.4
2,475,188 - dsdC b2364 0.50 -0.2
2,475,188 - dsdC b2364 0.50 -0.1
2,475,231 + dsdC b2364 0.55 +0.2
2,475,309 + dsdC b2364 0.63 +0.0
2,475,312 - dsdC b2364 0.64 +0.3
2,475,320 + dsdC b2364 0.65 +1.5
2,475,382 + dsdC b2364 0.71 +0.0
2,475,404 - dsdC b2364 0.74 -0.1
2,475,477 - dsdC b2364 0.81 +0.1
2,475,488 + dsdC b2364 0.82 -0.3

Or see this region's nucleotide sequence