Strain Fitness in Escherichia coli BW25113 around b3966

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrmA and btuB are separated by 368 nucleotidesbtuB and murI overlap by 56 nucleotides b3965: trmA - tRNA (uracil-5-)-methyltransferase (NCBI), at 4,160,193 to 4,161,293 trmA b3966: btuB - vitamin B12/cobalamin outer membrane transporter (NCBI), at 4,161,662 to 4,163,506 btuB b3967: murI - glutamate racemase, required for biosynthesis of D-glutamate and peptidoglycan (VIMSS), at 4,163,451 to 4,164,308 murI Position (kb) 4161 4162 4163 4164Strain fitness (log2 ratio) -2 -1 0 1 2at 4160.673 kb on + strand, within trmAat 4160.673 kb on + strand, within trmAat 4160.686 kb on + strand, within trmAat 4160.687 kb on - strand, within trmAat 4160.687 kb on - strand, within trmAat 4160.734 kb on - strand, within trmAat 4160.751 kb on - strand, within trmAat 4160.767 kb on - strand, within trmAat 4160.767 kb on - strand, within trmAat 4160.781 kb on + strand, within trmAat 4160.781 kb on - strand, within trmAat 4160.799 kb on - strand, within trmAat 4160.877 kb on - strand, within trmAat 4160.882 kb on - strand, within trmAat 4160.914 kb on + strand, within trmAat 4160.921 kb on + strand, within trmAat 4160.921 kb on - strand, within trmAat 4160.922 kb on + strand, within trmAat 4160.930 kb on - strand, within trmAat 4160.930 kb on - strand, within trmAat 4160.952 kb on - strand, within trmAat 4160.954 kb on + strand, within trmAat 4160.954 kb on + strand, within trmAat 4160.962 kb on - strand, within trmAat 4160.969 kb on - strand, within trmAat 4160.969 kb on - strand, within trmAat 4160.983 kb on - strand, within trmAat 4160.993 kb on + strand, within trmAat 4161.011 kb on - strand, within trmAat 4161.011 kb on - strand, within trmAat 4161.013 kb on + strand, within trmAat 4161.013 kb on + strand, within trmAat 4161.021 kb on - strand, within trmAat 4161.021 kb on - strand, within trmAat 4161.055 kb on - strand, within trmAat 4161.103 kb on + strand, within trmAat 4161.139 kb on + strand, within trmAat 4161.149 kb on - strand, within trmAat 4161.161 kb on + strand, within trmAat 4161.164 kb on - strand, within trmAat 4161.215 kb on + strandat 4161.223 kb on - strandat 4161.259 kb on + strandat 4161.264 kb on - strandat 4161.327 kb on + strandat 4161.337 kb on + strandat 4161.390 kb on - strandat 4161.436 kb on - strandat 4161.446 kb on + strandat 4161.448 kb on - strandat 4161.448 kb on - strandat 4161.537 kb on - strandat 4161.537 kb on - strandat 4161.538 kb on - strandat 4161.548 kb on - strandat 4161.548 kb on - strandat 4161.564 kb on + strandat 4161.564 kb on + strandat 4161.572 kb on - strandat 4161.609 kb on + strandat 4161.618 kb on - strandat 4161.618 kb on - strandat 4161.622 kb on - strandat 4161.622 kb on - strandat 4161.722 kb on - strandat 4161.741 kb on + strandat 4161.741 kb on + strandat 4161.783 kb on - strandat 4161.783 kb on - strandat 4161.791 kb on + strandat 4161.794 kb on - strandat 4161.826 kb on - strandat 4161.826 kb on - strandat 4161.836 kb on - strandat 4161.907 kb on + strand, within btuBat 4161.949 kb on + strand, within btuBat 4161.949 kb on + strand, within btuBat 4161.994 kb on - strand, within btuBat 4161.994 kb on - strand, within btuBat 4161.996 kb on - strand, within btuBat 4162.005 kb on + strand, within btuBat 4162.013 kb on - strand, within btuBat 4162.017 kb on + strand, within btuBat 4162.017 kb on + strand, within btuBat 4162.023 kb on - strand, within btuBat 4162.028 kb on - strand, within btuBat 4162.050 kb on + strand, within btuBat 4162.053 kb on - strand, within btuBat 4162.070 kb on + strand, within btuBat 4162.078 kb on - strand, within btuBat 4162.107 kb on - strand, within btuBat 4162.145 kb on + strand, within btuBat 4162.148 kb on + strand, within btuBat 4162.174 kb on + strand, within btuBat 4162.182 kb on + strand, within btuBat 4162.182 kb on + strand, within btuBat 4162.190 kb on + strand, within btuBat 4162.190 kb on - strand, within btuBat 4162.190 kb on - strand, within btuBat 4162.202 kb on + strand, within btuBat 4162.237 kb on + strand, within btuBat 4162.265 kb on + strand, within btuBat 4162.270 kb on + strand, within btuBat 4162.278 kb on - strand, within btuBat 4162.334 kb on + strand, within btuBat 4162.385 kb on + strand, within btuBat 4162.388 kb on - strand, within btuBat 4162.388 kb on - strand, within btuBat 4162.414 kb on + strand, within btuBat 4162.436 kb on + strand, within btuBat 4162.514 kb on + strand, within btuBat 4162.524 kb on + strand, within btuBat 4162.532 kb on - strand, within btuBat 4162.537 kb on - strand, within btuBat 4162.541 kb on + strand, within btuBat 4162.541 kb on + strand, within btuBat 4162.591 kb on + strand, within btuBat 4162.596 kb on + strand, within btuBat 4162.603 kb on + strand, within btuBat 4162.603 kb on + strand, within btuBat 4162.603 kb on + strand, within btuBat 4162.603 kb on + strand, within btuBat 4162.658 kb on - strand, within btuBat 4162.661 kb on + strand, within btuBat 4162.669 kb on - strand, within btuBat 4162.669 kb on - strand, within btuBat 4162.670 kb on - strand, within btuBat 4162.701 kb on - strand, within btuBat 4162.701 kb on - strand, within btuBat 4162.706 kb on + strand, within btuBat 4162.706 kb on + strand, within btuBat 4162.706 kb on + strand, within btuBat 4162.716 kb on + strand, within btuBat 4162.721 kb on + strand, within btuBat 4162.721 kb on + strand, within btuBat 4162.726 kb on + strand, within btuBat 4162.726 kb on + strand, within btuBat 4162.734 kb on - strand, within btuBat 4162.734 kb on - strand, within btuBat 4162.734 kb on - strand, within btuBat 4162.741 kb on + strand, within btuBat 4162.741 kb on + strand, within btuBat 4162.776 kb on + strand, within btuBat 4162.785 kb on + strand, within btuBat 4162.785 kb on + strand, within btuBat 4162.785 kb on + strand, within btuBat 4162.822 kb on + strand, within btuBat 4162.822 kb on + strand, within btuBat 4162.830 kb on - strand, within btuBat 4162.830 kb on - strand, within btuBat 4162.866 kb on + strand, within btuBat 4162.885 kb on + strand, within btuBat 4162.918 kb on - strand, within btuBat 4162.924 kb on + strand, within btuBat 4162.924 kb on + strand, within btuBat 4162.985 kb on + strand, within btuBat 4162.985 kb on + strand, within btuBat 4163.011 kb on - strand, within btuBat 4163.109 kb on - strand, within btuBat 4163.141 kb on + strand, within btuBat 4163.141 kb on + strand, within btuBat 4163.146 kb on + strand, within btuBat 4163.148 kb on + strand, within btuBat 4163.156 kb on - strand, within btuBat 4163.168 kb on - strand, within btuBat 4163.212 kb on - strand, within btuBat 4163.212 kb on - strand, within btuBat 4163.214 kb on + strand, within btuBat 4163.214 kb on + strand, within btuBat 4163.269 kb on + strand, within btuBat 4163.269 kb on + strand, within btuBat 4163.272 kb on - strand, within btuBat 4163.272 kb on - strand, within btuBat 4163.317 kb on + strand, within btuBat 4163.357 kb on - strandat 4163.357 kb on - strandat 4163.357 kb on - strandat 4163.388 kb on + strandat 4163.388 kb on + strandat 4163.447 kb on + strandat 4163.447 kb on + strandat 4163.455 kb on - strandat 4163.455 kb on - strandat 4163.455 kb on - strandat 4163.455 kb on - strandat 4163.455 kb on - strandat 4163.505 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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4,160,673 + trmA b3965 0.44 +0.4
4,160,673 + trmA b3965 0.44 +0.3
4,160,686 + trmA b3965 0.45 +0.0
4,160,687 - trmA b3965 0.45 -0.6
4,160,687 - trmA b3965 0.45 +0.4
4,160,734 - trmA b3965 0.49 +1.3
4,160,751 - trmA b3965 0.51 +0.3
4,160,767 - trmA b3965 0.52 +0.1
4,160,767 - trmA b3965 0.52 +0.5
4,160,781 + trmA b3965 0.53 +0.3
4,160,781 - trmA b3965 0.53 +0.1
4,160,799 - trmA b3965 0.55 -0.7
4,160,877 - trmA b3965 0.62 -0.4
4,160,882 - trmA b3965 0.63 +0.1
4,160,914 + trmA b3965 0.65 -1.3
4,160,921 + trmA b3965 0.66 -0.0
4,160,921 - trmA b3965 0.66 -0.7
4,160,922 + trmA b3965 0.66 +0.0
4,160,930 - trmA b3965 0.67 -0.1
4,160,930 - trmA b3965 0.67 +1.3
4,160,952 - trmA b3965 0.69 +0.2
4,160,954 + trmA b3965 0.69 +2.2
4,160,954 + trmA b3965 0.69 +0.6
4,160,962 - trmA b3965 0.70 -0.0
4,160,969 - trmA b3965 0.70 +0.4
4,160,969 - trmA b3965 0.70 +0.7
4,160,983 - trmA b3965 0.72 +0.9
4,160,993 + trmA b3965 0.73 +0.1
4,161,011 - trmA b3965 0.74 +0.0
4,161,011 - trmA b3965 0.74 -0.3
4,161,013 + trmA b3965 0.74 +0.2
4,161,013 + trmA b3965 0.74 +0.1
4,161,021 - trmA b3965 0.75 -0.6
4,161,021 - trmA b3965 0.75 +0.5
4,161,055 - trmA b3965 0.78 +0.5
4,161,103 + trmA b3965 0.83 -0.1
4,161,139 + trmA b3965 0.86 +0.3
4,161,149 - trmA b3965 0.87 +0.3
4,161,161 + trmA b3965 0.88 +0.4
4,161,164 - trmA b3965 0.88 -0.0
4,161,215 + +0.3
4,161,223 - +0.3
4,161,259 + -0.3
4,161,264 - -1.0
4,161,327 + +0.6
4,161,337 + -0.0
4,161,390 - +0.4
4,161,436 - +0.5
4,161,446 + -0.4
4,161,448 - +0.9
4,161,448 - +0.4
4,161,537 - -0.0
4,161,537 - +0.4
4,161,538 - -0.7
4,161,548 - -0.7
4,161,548 - +0.5
4,161,564 + -0.8
4,161,564 + -0.6
4,161,572 - -0.3
4,161,609 + +0.2
4,161,618 - +0.3
4,161,618 - +0.1
4,161,622 - -0.1
4,161,622 - -0.1
4,161,722 - +0.1
4,161,741 + -0.5
4,161,741 + +0.3
4,161,783 - -0.2
4,161,783 - +0.7
4,161,791 + -0.4
4,161,794 - +0.9
4,161,826 - -0.1
4,161,826 - -0.6
4,161,836 - +0.4
4,161,907 + btuB b3966 0.13 -0.0
4,161,949 + btuB b3966 0.16 +0.7
4,161,949 + btuB b3966 0.16 +0.5
4,161,994 - btuB b3966 0.18 +0.2
4,161,994 - btuB b3966 0.18 +0.4
4,161,996 - btuB b3966 0.18 +0.6
4,162,005 + btuB b3966 0.19 +0.2
4,162,013 - btuB b3966 0.19 +0.2
4,162,017 + btuB b3966 0.19 +0.3
4,162,017 + btuB b3966 0.19 +0.3
4,162,023 - btuB b3966 0.20 +1.1
4,162,028 - btuB b3966 0.20 +0.3
4,162,050 + btuB b3966 0.21 +0.7
4,162,053 - btuB b3966 0.21 +0.9
4,162,070 + btuB b3966 0.22 -0.5
4,162,078 - btuB b3966 0.23 +0.1
4,162,107 - btuB b3966 0.24 +0.9
4,162,145 + btuB b3966 0.26 +0.2
4,162,148 + btuB b3966 0.26 +0.3
4,162,174 + btuB b3966 0.28 +0.3
4,162,182 + btuB b3966 0.28 +0.3
4,162,182 + btuB b3966 0.28 -0.3
4,162,190 + btuB b3966 0.29 -0.1
4,162,190 - btuB b3966 0.29 +0.1
4,162,190 - btuB b3966 0.29 +0.6
4,162,202 + btuB b3966 0.29 +0.0
4,162,237 + btuB b3966 0.31 -0.1
4,162,265 + btuB b3966 0.33 +0.1
4,162,270 + btuB b3966 0.33 +0.5
4,162,278 - btuB b3966 0.33 +0.6
4,162,334 + btuB b3966 0.36 +1.1
4,162,385 + btuB b3966 0.39 +0.0
4,162,388 - btuB b3966 0.39 +0.7
4,162,388 - btuB b3966 0.39 +1.4
4,162,414 + btuB b3966 0.41 +0.2
4,162,436 + btuB b3966 0.42 -0.4
4,162,514 + btuB b3966 0.46 +0.9
4,162,524 + btuB b3966 0.47 +0.4
4,162,532 - btuB b3966 0.47 -0.0
4,162,537 - btuB b3966 0.47 +0.6
4,162,541 + btuB b3966 0.48 +0.8
4,162,541 + btuB b3966 0.48 +0.0
4,162,591 + btuB b3966 0.50 -0.1
4,162,596 + btuB b3966 0.51 +0.4
4,162,603 + btuB b3966 0.51 +0.5
4,162,603 + btuB b3966 0.51 +1.4
4,162,603 + btuB b3966 0.51 -0.9
4,162,603 + btuB b3966 0.51 +0.2
4,162,658 - btuB b3966 0.54 +1.5
4,162,661 + btuB b3966 0.54 +0.0
4,162,669 - btuB b3966 0.55 +0.3
4,162,669 - btuB b3966 0.55 -0.6
4,162,670 - btuB b3966 0.55 -0.9
4,162,701 - btuB b3966 0.56 +0.9
4,162,701 - btuB b3966 0.56 -0.1
4,162,706 + btuB b3966 0.57 -0.3
4,162,706 + btuB b3966 0.57 +0.4
4,162,706 + btuB b3966 0.57 +0.6
4,162,716 + btuB b3966 0.57 +0.4
4,162,721 + btuB b3966 0.57 +1.2
4,162,721 + btuB b3966 0.57 +0.3
4,162,726 + btuB b3966 0.58 -0.2
4,162,726 + btuB b3966 0.58 +0.1
4,162,734 - btuB b3966 0.58 +0.6
4,162,734 - btuB b3966 0.58 +0.5
4,162,734 - btuB b3966 0.58 +1.1
4,162,741 + btuB b3966 0.58 -0.6
4,162,741 + btuB b3966 0.58 -1.3
4,162,776 + btuB b3966 0.60 +0.2
4,162,785 + btuB b3966 0.61 +1.2
4,162,785 + btuB b3966 0.61 +0.7
4,162,785 + btuB b3966 0.61 +0.0
4,162,822 + btuB b3966 0.63 +0.1
4,162,822 + btuB b3966 0.63 +0.8
4,162,830 - btuB b3966 0.63 +0.3
4,162,830 - btuB b3966 0.63 +0.0
4,162,866 + btuB b3966 0.65 -1.4
4,162,885 + btuB b3966 0.66 -0.1
4,162,918 - btuB b3966 0.68 +0.2
4,162,924 + btuB b3966 0.68 +0.6
4,162,924 + btuB b3966 0.68 +0.1
4,162,985 + btuB b3966 0.72 -0.8
4,162,985 + btuB b3966 0.72 -0.1
4,163,011 - btuB b3966 0.73 +0.5
4,163,109 - btuB b3966 0.78 +1.4
4,163,141 + btuB b3966 0.80 +0.5
4,163,141 + btuB b3966 0.80 +1.0
4,163,146 + btuB b3966 0.80 +0.1
4,163,148 + btuB b3966 0.81 -0.6
4,163,156 - btuB b3966 0.81 +0.1
4,163,168 - btuB b3966 0.82 -0.0
4,163,212 - btuB b3966 0.84 +0.2
4,163,212 - btuB b3966 0.84 +1.6
4,163,214 + btuB b3966 0.84 +0.5
4,163,214 + btuB b3966 0.84 +0.6
4,163,269 + btuB b3966 0.87 +0.7
4,163,269 + btuB b3966 0.87 +0.9
4,163,272 - btuB b3966 0.87 -0.8
4,163,272 - btuB b3966 0.87 +0.1
4,163,317 + btuB b3966 0.90 +0.4
4,163,357 - +0.9
4,163,357 - -1.4
4,163,357 - +0.4
4,163,388 + +1.1
4,163,388 + +0.6
4,163,447 + -0.9
4,163,447 + +0.4
4,163,455 - +0.3
4,163,455 - +1.1
4,163,455 - +0.1
4,163,455 - +0.7
4,163,455 - +0.6
4,163,505 + -1.9

Or see this region's nucleotide sequence