Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dcuD and sspB are separated by 42 nucleotides sspB and sspA are separated by 5 nucleotides sspA and rpsI are separated by 394 nucleotides rpsI and rplM are separated by 15 nucleotides
b3227: dcuD - predicted transporter (NCBI), at 3,372,891 to 3,374,258
dcuD
b3228: sspB - ClpXP protease specificity-enhancing factor (NCBI), at 3,374,301 to 3,374,798
sspB
b3229: sspA - stringent starvation protein A (NCBI), at 3,374,804 to 3,375,442
sspA
b3230: rpsI - 30S ribosomal protein S9 (NCBI), at 3,375,837 to 3,376,229
rpsI
b3231: rplM - 50S ribosomal protein L13 (NCBI), at 3,376,245 to 3,376,673
rplM
Position (kb)
3374
3375
3376 Strain fitness (log2 ratio)
-2
-1
0
1 at 3373.896 kb on + strand, within dcuD at 3373.897 kb on - strand, within dcuD at 3373.924 kb on + strand, within dcuD at 3373.924 kb on + strand, within dcuD at 3373.932 kb on - strand, within dcuD at 3373.932 kb on - strand, within dcuD at 3373.933 kb on - strand at 3373.933 kb on - strand, within dcuD at 3373.984 kb on + strand, within dcuD at 3374.004 kb on - strand, within dcuD at 3374.052 kb on + strand, within dcuD at 3374.060 kb on - strand, within dcuD at 3374.103 kb on + strand, within dcuD at 3374.128 kb on + strand at 3374.209 kb on - strand at 3374.249 kb on + strand at 3374.249 kb on + strand at 3374.306 kb on - strand at 3374.306 kb on - strand at 3374.366 kb on - strand, within sspB at 3374.472 kb on - strand, within sspB at 3374.472 kb on - strand, within sspB at 3374.484 kb on - strand, within sspB at 3374.484 kb on - strand, within sspB at 3374.514 kb on + strand, within sspB at 3374.543 kb on - strand, within sspB at 3374.543 kb on - strand, within sspB at 3374.563 kb on + strand, within sspB at 3374.667 kb on + strand, within sspB at 3374.667 kb on + strand, within sspB at 3374.675 kb on - strand, within sspB at 3374.675 kb on - strand, within sspB at 3374.684 kb on + strand, within sspB at 3374.684 kb on + strand, within sspB at 3374.966 kb on - strand, within sspA at 3374.976 kb on + strand, within sspA at 3374.976 kb on + strand, within sspA at 3375.071 kb on - strand, within sspA at 3375.073 kb on - strand, within sspA at 3375.084 kb on - strand, within sspA at 3375.098 kb on + strand, within sspA at 3375.098 kb on + strand, within sspA at 3375.113 kb on - strand, within sspA at 3375.189 kb on - strand, within sspA at 3375.220 kb on - strand, within sspA at 3375.220 kb on - strand, within sspA at 3375.220 kb on - strand, within sspA at 3375.236 kb on - strand, within sspA at 3375.353 kb on + strand, within sspA at 3375.353 kb on + strand, within sspA at 3375.515 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 3,373,896 + dcuD b3227 0.73 -0.1 3,373,897 - dcuD b3227 0.74 -0.1 3,373,924 + dcuD b3227 0.76 -0.7 3,373,924 + dcuD b3227 0.76 +0.5 3,373,932 - dcuD b3227 0.76 +1.0 3,373,932 - dcuD b3227 0.76 -1.8 3,373,933 - -0.5 3,373,933 - dcuD b3227 0.76 -2.0 3,373,984 + dcuD b3227 0.80 -0.2 3,374,004 - dcuD b3227 0.81 +0.1 3,374,052 + dcuD b3227 0.85 +0.8 3,374,060 - dcuD b3227 0.85 -1.7 3,374,103 + dcuD b3227 0.89 -0.8 3,374,128 + -0.6 3,374,209 - +0.7 3,374,249 + -0.7 3,374,249 + +0.2 3,374,306 - -0.5 3,374,306 - -0.6 3,374,366 - sspB b3228 0.13 -0.3 3,374,472 - sspB b3228 0.34 -0.6 3,374,472 - sspB b3228 0.34 -0.1 3,374,484 - sspB b3228 0.37 +0.3 3,374,484 - sspB b3228 0.37 -0.5 3,374,514 + sspB b3228 0.43 +0.8 3,374,543 - sspB b3228 0.49 -0.5 3,374,543 - sspB b3228 0.49 -1.1 3,374,563 + sspB b3228 0.53 -0.6 3,374,667 + sspB b3228 0.73 -0.2 3,374,667 + sspB b3228 0.73 -0.5 3,374,675 - sspB b3228 0.75 +0.8 3,374,675 - sspB b3228 0.75 -0.6 3,374,684 + sspB b3228 0.77 +0.2 3,374,684 + sspB b3228 0.77 +0.3 3,374,966 - sspA b3229 0.25 -1.5 3,374,976 + sspA b3229 0.27 -0.2 3,374,976 + sspA b3229 0.27 -1.5 3,375,071 - sspA b3229 0.42 +0.2 3,375,073 - sspA b3229 0.42 -0.8 3,375,084 - sspA b3229 0.44 -1.9 3,375,098 + sspA b3229 0.46 -0.8 3,375,098 + sspA b3229 0.46 +0.4 3,375,113 - sspA b3229 0.48 -1.8 3,375,189 - sspA b3229 0.60 -2.4 3,375,220 - sspA b3229 0.65 -1.5 3,375,220 - sspA b3229 0.65 -0.8 3,375,220 - sspA b3229 0.65 -0.5 3,375,236 - sspA b3229 0.68 +0.2 3,375,353 + sspA b3229 0.86 -0.2 3,375,353 + sspA b3229 0.86 -1.8 3,375,515 - -0.7
Or see this region's nucleotide sequence