Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yqgA and pheV are separated by 105 nucleotides pheV and yghD are separated by 148 nucleotides yghD and yghE are separated by 1 nucleotides yghE and yghF are separated by 2 nucleotides yghF and yghG are separated by 125 nucleotides
b2966: yqgA - predicted inner membrane protein (NCBI), at 3,107,575 to 3,108,282
yqgA
b2967: pheV - tRNA-Phe (NCBI), at 3,108,388 to 3,108,463
pheV
b2968: yghD - predicted secretion pathway M-type protein, membrane anchored (NCBI), at 3,108,612 to 3,109,148
yghD
b2969: yghE - predicted secretion pathway protein, L-type protein (pseudogene) (RefSeq), at 3,109,150 to 3,110,112
yghE
b2970: yghF - predicted secretion pathway protein, C-type protein (NCBI), at 3,110,115 to 3,110,963
yghF
b2971: yghG - hypothetical protein (NCBI), at 3,111,089 to 3,111,499
yghG
Position (kb)
3109
3110
3111 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3108.160 kb on + strand, within yqgA at 3108.181 kb on - strand, within yqgA at 3108.221 kb on - strand at 3108.222 kb on + strand at 3108.222 kb on + strand at 3108.378 kb on - strand at 3108.378 kb on - strand at 3108.593 kb on + strand at 3108.629 kb on - strand at 3108.629 kb on - strand at 3108.649 kb on + strand at 3108.766 kb on + strand, within yghD at 3108.821 kb on + strand, within yghD at 3108.833 kb on - strand, within yghD at 3108.833 kb on - strand, within yghD at 3108.878 kb on + strand, within yghD at 3108.931 kb on - strand, within yghD at 3108.940 kb on + strand, within yghD at 3108.940 kb on + strand, within yghD at 3109.078 kb on - strand, within yghD at 3109.098 kb on - strand at 3109.123 kb on - strand at 3109.174 kb on + strand at 3109.220 kb on - strand at 3109.437 kb on + strand, within yghE at 3109.538 kb on + strand, within yghE at 3109.551 kb on + strand, within yghE at 3109.704 kb on + strand, within yghE at 3109.704 kb on + strand, within yghE at 3109.712 kb on - strand, within yghE at 3109.886 kb on - strand, within yghE at 3109.933 kb on - strand, within yghE at 3110.044 kb on - strand at 3110.073 kb on + strand at 3110.073 kb on + strand at 3110.085 kb on - strand at 3110.085 kb on - strand at 3110.184 kb on + strand at 3110.209 kb on - strand, within yghF at 3110.231 kb on - strand, within yghF at 3110.296 kb on - strand, within yghF at 3110.328 kb on + strand, within yghF at 3110.328 kb on + strand, within yghF at 3110.329 kb on - strand, within yghF at 3110.342 kb on - strand, within yghF at 3110.426 kb on + strand, within yghF at 3110.427 kb on + strand, within yghF at 3110.427 kb on + strand, within yghF at 3110.507 kb on + strand, within yghF at 3110.550 kb on - strand, within yghF at 3110.622 kb on - strand, within yghF at 3110.622 kb on - strand, within yghF at 3110.664 kb on + strand, within yghF at 3110.723 kb on + strand, within yghF at 3110.732 kb on + strand, within yghF at 3110.741 kb on + strand, within yghF at 3110.819 kb on + strand, within yghF at 3110.826 kb on - strand, within yghF at 3110.855 kb on + strand, within yghF at 3110.855 kb on + strand, within yghF at 3110.855 kb on + strand, within yghF at 3110.857 kb on + strand, within yghF at 3110.865 kb on - strand, within yghF at 3110.877 kb on + strand, within yghF at 3110.877 kb on + strand, within yghF at 3110.886 kb on + strand at 3110.886 kb on + strand at 3110.888 kb on + strand at 3110.888 kb on + strand at 3110.894 kb on - strand at 3110.935 kb on - strand at 3110.935 kb on - strand at 3110.977 kb on + strand at 3110.977 kb on + strand at 3110.986 kb on - strand at 3110.994 kb on + strand at 3110.996 kb on + strand at 3111.031 kb on - strand at 3111.031 kb on - strand at 3111.070 kb on + strand at 3111.086 kb on + strand at 3111.102 kb on + strand at 3111.103 kb on + strand at 3111.108 kb on - strand at 3111.108 kb on - strand at 3111.110 kb on - strand at 3111.110 kb on - strand at 3111.111 kb on - strand at 3111.111 kb on - strand at 3111.111 kb on - strand at 3111.111 kb on - strand at 3111.111 kb on - strand at 3111.111 kb on - strand at 3111.111 kb on - strand at 3111.111 kb on - strand at 3111.111 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 3,108,160 + yqgA b2966 0.83 -0.1 3,108,181 - yqgA b2966 0.86 +0.1 3,108,221 - -0.7 3,108,222 + -0.3 3,108,222 + +0.8 3,108,378 - -0.3 3,108,378 - +0.1 3,108,593 + +0.9 3,108,629 - +1.2 3,108,629 - -0.1 3,108,649 + +0.4 3,108,766 + yghD b2968 0.29 -0.2 3,108,821 + yghD b2968 0.39 -0.4 3,108,833 - yghD b2968 0.41 +0.5 3,108,833 - yghD b2968 0.41 -0.5 3,108,878 + yghD b2968 0.50 +0.7 3,108,931 - yghD b2968 0.59 -0.2 3,108,940 + yghD b2968 0.61 -0.0 3,108,940 + yghD b2968 0.61 +0.3 3,109,078 - yghD b2968 0.87 -0.7 3,109,098 - -0.0 3,109,123 - -0.0 3,109,174 + -0.4 3,109,220 - -0.9 3,109,437 + yghE b2969 0.30 +1.5 3,109,538 + yghE b2969 0.40 +0.3 3,109,551 + yghE b2969 0.42 +1.4 3,109,704 + yghE b2969 0.58 +0.4 3,109,704 + yghE b2969 0.58 -0.0 3,109,712 - yghE b2969 0.58 -0.9 3,109,886 - yghE b2969 0.76 -0.1 3,109,933 - yghE b2969 0.81 +0.7 3,110,044 - -0.3 3,110,073 + +0.9 3,110,073 + +0.6 3,110,085 - -0.8 3,110,085 - -0.0 3,110,184 + -0.3 3,110,209 - yghF b2970 0.11 -0.1 3,110,231 - yghF b2970 0.14 -0.0 3,110,296 - yghF b2970 0.21 -0.4 3,110,328 + yghF b2970 0.25 -0.4 3,110,328 + yghF b2970 0.25 -0.5 3,110,329 - yghF b2970 0.25 -0.6 3,110,342 - yghF b2970 0.27 -0.2 3,110,426 + yghF b2970 0.37 +0.6 3,110,427 + yghF b2970 0.37 -0.0 3,110,427 + yghF b2970 0.37 -0.0 3,110,507 + yghF b2970 0.46 +0.2 3,110,550 - yghF b2970 0.51 +0.2 3,110,622 - yghF b2970 0.60 -0.5 3,110,622 - yghF b2970 0.60 -0.4 3,110,664 + yghF b2970 0.65 -0.3 3,110,723 + yghF b2970 0.72 -0.2 3,110,732 + yghF b2970 0.73 -0.4 3,110,741 + yghF b2970 0.74 +0.3 3,110,819 + yghF b2970 0.83 -0.2 3,110,826 - yghF b2970 0.84 -0.4 3,110,855 + yghF b2970 0.87 -0.6 3,110,855 + yghF b2970 0.87 +1.0 3,110,855 + yghF b2970 0.87 +1.0 3,110,857 + yghF b2970 0.87 +0.3 3,110,865 - yghF b2970 0.88 +0.5 3,110,877 + yghF b2970 0.90 +1.0 3,110,877 + yghF b2970 0.90 -1.1 3,110,886 + -0.1 3,110,886 + -0.6 3,110,888 + -0.5 3,110,888 + -0.9 3,110,894 - +1.7 3,110,935 - +2.7 3,110,935 - -2.1 3,110,977 + -0.2 3,110,977 + +1.0 3,110,986 - +0.7 3,110,994 + -2.1 3,110,996 + -0.6 3,111,031 - +0.5 3,111,031 - +0.2 3,111,070 + -0.3 3,111,086 + +2.7 3,111,102 + +0.7 3,111,103 + +1.3 3,111,108 - +0.1 3,111,108 - -0.5 3,111,110 - -0.0 3,111,110 - +0.7 3,111,111 - -0.4 3,111,111 - +1.4 3,111,111 - +1.3 3,111,111 - +0.5 3,111,111 - +1.1 3,111,111 - +0.1 3,111,111 - -0.6 3,111,111 - +1.5 3,111,111 - -1.3
Or see this region's nucleotide sequence