Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt speA and yqgB are separated by 8 nucleotides yqgB and yqgC are separated by 135 nucleotides yqgC and metK are separated by 303 nucleotides
b2938: speA - arginine decarboxylase (NCBI), at 3,081,957 to 3,083,933
speA
b2939: yqgB - predicted protein (RefSeq), at 3,083,942 to 3,084,073
yqgB
b2940: yqgC - hypothetical protein (NCBI), at 3,084,209 to 3,084,424
yqgC
b2942: metK - S-adenosylmethionine synthetase (NCBI), at 3,084,728 to 3,085,882
metK
Position (kb)
3083
3084
3085 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3083.018 kb on + strand, within speA at 3083.040 kb on + strand, within speA at 3083.040 kb on + strand, within speA at 3083.045 kb on + strand, within speA at 3083.045 kb on + strand, within speA at 3083.142 kb on + strand, within speA at 3083.142 kb on + strand, within speA at 3083.287 kb on - strand, within speA at 3083.287 kb on - strand, within speA at 3083.305 kb on + strand, within speA at 3083.305 kb on + strand, within speA at 3083.361 kb on + strand, within speA at 3083.361 kb on + strand, within speA at 3083.365 kb on - strand, within speA at 3083.365 kb on - strand, within speA at 3083.408 kb on + strand, within speA at 3083.416 kb on - strand, within speA at 3083.440 kb on - strand, within speA at 3083.440 kb on - strand, within speA at 3083.452 kb on - strand, within speA at 3083.452 kb on - strand, within speA at 3083.462 kb on - strand, within speA at 3083.575 kb on - strand, within speA at 3083.575 kb on - strand, within speA at 3083.618 kb on - strand, within speA at 3083.677 kb on - strand, within speA at 3083.693 kb on + strand, within speA at 3083.693 kb on + strand, within speA at 3083.761 kb on - strand at 3083.772 kb on - strand at 3083.802 kb on - strand at 3083.823 kb on + strand at 3083.834 kb on - strand at 3083.834 kb on - strand at 3083.872 kb on + strand at 3083.874 kb on + strand at 3083.880 kb on - strand at 3083.880 kb on - strand at 3083.911 kb on + strand at 3083.985 kb on + strand, within yqgB at 3084.042 kb on + strand, within yqgB at 3084.150 kb on - strand at 3084.153 kb on + strand at 3084.166 kb on - strand at 3084.181 kb on - strand at 3084.227 kb on + strand at 3084.242 kb on + strand, within yqgC at 3084.259 kb on - strand, within yqgC at 3084.259 kb on - strand, within yqgC at 3084.259 kb on - strand, within yqgC at 3084.285 kb on - strand, within yqgC at 3084.345 kb on - strand, within yqgC at 3084.471 kb on + strand at 3084.632 kb on + strand at 3084.632 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 3,083,018 + speA b2938 0.54 -1.3 3,083,040 + speA b2938 0.55 -0.3 3,083,040 + speA b2938 0.55 -1.7 3,083,045 + speA b2938 0.55 +0.1 3,083,045 + speA b2938 0.55 -2.1 3,083,142 + speA b2938 0.60 +2.7 3,083,142 + speA b2938 0.60 -2.0 3,083,287 - speA b2938 0.67 -0.1 3,083,287 - speA b2938 0.67 -1.6 3,083,305 + speA b2938 0.68 -0.8 3,083,305 + speA b2938 0.68 -0.6 3,083,361 + speA b2938 0.71 +0.3 3,083,361 + speA b2938 0.71 -1.3 3,083,365 - speA b2938 0.71 -1.1 3,083,365 - speA b2938 0.71 +0.7 3,083,408 + speA b2938 0.73 -1.4 3,083,416 - speA b2938 0.74 -1.6 3,083,440 - speA b2938 0.75 -1.4 3,083,440 - speA b2938 0.75 +0.5 3,083,452 - speA b2938 0.76 -0.1 3,083,452 - speA b2938 0.76 -2.0 3,083,462 - speA b2938 0.76 -1.9 3,083,575 - speA b2938 0.82 -0.6 3,083,575 - speA b2938 0.82 -1.1 3,083,618 - speA b2938 0.84 -0.9 3,083,677 - speA b2938 0.87 -1.5 3,083,693 + speA b2938 0.88 -2.6 3,083,693 + speA b2938 0.88 -0.9 3,083,761 - -1.8 3,083,772 - -0.9 3,083,802 - -2.0 3,083,823 + -1.9 3,083,834 - -1.2 3,083,834 - -1.1 3,083,872 + -0.1 3,083,874 + +0.3 3,083,880 - +0.0 3,083,880 - -0.3 3,083,911 + -1.1 3,083,985 + yqgB b2939 0.33 -1.3 3,084,042 + yqgB b2939 0.76 -1.8 3,084,150 - +0.2 3,084,153 + -0.3 3,084,166 - +0.7 3,084,181 - +0.3 3,084,227 + -1.4 3,084,242 + yqgC b2940 0.15 -1.2 3,084,259 - yqgC b2940 0.23 +0.3 3,084,259 - yqgC b2940 0.23 +0.3 3,084,259 - yqgC b2940 0.23 +0.3 3,084,285 - yqgC b2940 0.35 +0.9 3,084,345 - yqgC b2940 0.63 -0.3 3,084,471 + -0.2 3,084,632 + -0.5 3,084,632 + -0.5
Or see this region's nucleotide sequence