Strain Fitness in Escherichia coli BW25113 around b2711

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnorV and norW overlap by 4 nucleotidesnorW and hypF are separated by 127 nucleotides b2710: norV - anaerobic nitric oxide reductase flavorubredoxin (NCBI), at 2,830,498 to 2,831,937 norV b2711: norW - nitric oxide reductase (NCBI), at 2,831,934 to 2,833,067 norW b2712: hypF - carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases (NCBI), at 2,833,195 to 2,835,447 hypF Position (kb) 2831 2832 2833 2834Strain fitness (log2 ratio) -1 0 1 2at 2830.983 kb on + strand, within norVat 2830.986 kb on - strand, within norVat 2830.986 kb on - strand, within norVat 2831.011 kb on - strand, within norVat 2831.011 kb on - strand, within norVat 2831.018 kb on - strand, within norVat 2831.018 kb on - strand, within norVat 2831.075 kb on - strand, within norVat 2831.075 kb on - strand, within norVat 2831.092 kb on - strand, within norVat 2831.109 kb on + strand, within norVat 2831.134 kb on + strand, within norVat 2831.273 kb on + strand, within norVat 2831.273 kb on + strand, within norVat 2831.314 kb on + strand, within norVat 2831.314 kb on + strand, within norVat 2831.629 kb on + strand, within norVat 2831.646 kb on - strand, within norVat 2831.646 kb on - strand, within norVat 2831.651 kb on + strand, within norVat 2831.706 kb on - strand, within norVat 2831.732 kb on - strand, within norVat 2831.748 kb on + strand, within norVat 2831.748 kb on + strand, within norVat 2831.829 kb on + strandat 2831.934 kb on - strandat 2831.957 kb on - strandat 2832.048 kb on + strand, within norWat 2832.064 kb on - strand, within norWat 2832.150 kb on - strand, within norWat 2832.281 kb on + strand, within norWat 2832.281 kb on + strand, within norWat 2832.364 kb on + strand, within norWat 2832.384 kb on - strand, within norWat 2832.384 kb on - strand, within norWat 2832.526 kb on + strand, within norWat 2832.896 kb on - strand, within norWat 2832.956 kb on + strandat 2833.005 kb on + strandat 2833.005 kb on + strandat 2833.046 kb on + strandat 2833.054 kb on - strandat 2833.119 kb on + strandat 2833.119 kb on + strandat 2833.350 kb on + strandat 2833.350 kb on + strandat 2833.353 kb on - strandat 2833.371 kb on + strandat 2833.399 kb on - strandat 2833.399 kb on - strandat 2833.408 kb on + strandat 2833.423 kb on + strand, within hypFat 2833.423 kb on + strand, within hypFat 2833.581 kb on + strand, within hypFat 2833.699 kb on + strand, within hypFat 2833.734 kb on + strand, within hypFat 2833.934 kb on - strand, within hypFat 2833.962 kb on + strand, within hypFat 2833.962 kb on + strand, within hypFat 2833.980 kb on - strand, within hypF

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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2,830,983 + norV b2710 0.34 -0.7
2,830,986 - norV b2710 0.34 -0.4
2,830,986 - norV b2710 0.34 -0.7
2,831,011 - norV b2710 0.36 +0.4
2,831,011 - norV b2710 0.36 +0.4
2,831,018 - norV b2710 0.36 -0.5
2,831,018 - norV b2710 0.36 -0.2
2,831,075 - norV b2710 0.40 +0.5
2,831,075 - norV b2710 0.40 +0.1
2,831,092 - norV b2710 0.41 -0.1
2,831,109 + norV b2710 0.42 +0.3
2,831,134 + norV b2710 0.44 -0.0
2,831,273 + norV b2710 0.54 +0.1
2,831,273 + norV b2710 0.54 -0.1
2,831,314 + norV b2710 0.57 +0.7
2,831,314 + norV b2710 0.57 +0.8
2,831,629 + norV b2710 0.79 -0.2
2,831,646 - norV b2710 0.80 -0.8
2,831,646 - norV b2710 0.80 -0.2
2,831,651 + norV b2710 0.80 +0.1
2,831,706 - norV b2710 0.84 -0.7
2,831,732 - norV b2710 0.86 -0.2
2,831,748 + norV b2710 0.87 +0.5
2,831,748 + norV b2710 0.87 +0.0
2,831,829 + +0.1
2,831,934 - +0.5
2,831,957 - +0.9
2,832,048 + norW b2711 0.10 -0.0
2,832,064 - norW b2711 0.11 +0.7
2,832,150 - norW b2711 0.19 -0.1
2,832,281 + norW b2711 0.31 -0.1
2,832,281 + norW b2711 0.31 +0.2
2,832,364 + norW b2711 0.38 +2.2
2,832,384 - norW b2711 0.40 +0.6
2,832,384 - norW b2711 0.40 +1.2
2,832,526 + norW b2711 0.52 +1.2
2,832,896 - norW b2711 0.85 +0.1
2,832,956 + +0.1
2,833,005 + +0.3
2,833,005 + -0.1
2,833,046 + -0.1
2,833,054 - +0.2
2,833,119 + -0.2
2,833,119 + +1.2
2,833,350 + -0.2
2,833,350 + -0.5
2,833,353 - +0.3
2,833,371 + -0.1
2,833,399 - -0.3
2,833,399 - +0.5
2,833,408 + -0.3
2,833,423 + hypF b2712 0.10 +0.5
2,833,423 + hypF b2712 0.10 -0.3
2,833,581 + hypF b2712 0.17 +1.0
2,833,699 + hypF b2712 0.22 +0.4
2,833,734 + hypF b2712 0.24 +0.1
2,833,934 - hypF b2712 0.33 +0.2
2,833,962 + hypF b2712 0.34 +0.1
2,833,962 + hypF b2712 0.34 -0.1
2,833,980 - hypF b2712 0.35 -0.8

Or see this region's nucleotide sequence