Strain Fitness in Escherichia coli BW25113 around b2209

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnapD and napF overlap by 11 nucleotidesnapF and yojO are separated by 108 nucleotidesyojO and eco are separated by 134 nucleotideseco and mqo are separated by 714 nucleotides b2207: napD - assembly protein for periplasmic nitrate reductase (NCBI), at 2,300,772 to 2,301,035 napD b2208: napF - ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI), at 2,301,025 to 2,301,519 napF b4604: yojO - hypothetical protein (RefSeq), at 2,301,628 to 2,301,792 yojO b2209: eco - ecotin precursor (NCBI), at 2,301,927 to 2,302,415 eco b2210: mqo - malate:quinone oxidoreductase (NCBI), at 2,303,130 to 2,304,776 mqo Position (kb) 2301 2302 2303Strain fitness (log2 ratio) -1 0 1at 2300.965 kb on + strand, within napDat 2301.037 kb on + strandat 2301.037 kb on + strandat 2301.060 kb on + strandat 2301.060 kb on + strandat 2301.084 kb on + strand, within napFat 2301.183 kb on - strand, within napFat 2301.210 kb on - strand, within napFat 2301.210 kb on - strand, within napFat 2301.256 kb on - strand, within napFat 2301.257 kb on - strand, within napFat 2301.286 kb on + strand, within napFat 2301.415 kb on - strand, within napFat 2301.496 kb on - strandat 2301.620 kb on + strandat 2301.874 kb on + strandat 2302.083 kb on + strand, within ecoat 2302.294 kb on + strand, within ecoat 2302.310 kb on - strand, within ecoat 2302.522 kb on + strandat 2302.748 kb on + strandat 2302.763 kb on + strandat 2302.763 kb on + strandat 2302.773 kb on + strandat 2303.223 kb on - strandat 2303.225 kb on + strandat 2303.226 kb on + strandat 2303.244 kb on + strandat 2303.346 kb on - strand, within mqoat 2303.346 kb on - strand, within mqo

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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2,300,965 + napD b2207 0.73 +0.7
2,301,037 + +0.2
2,301,037 + +1.0
2,301,060 + -0.0
2,301,060 + +0.9
2,301,084 + napF b2208 0.12 +0.6
2,301,183 - napF b2208 0.32 +0.6
2,301,210 - napF b2208 0.37 -0.7
2,301,210 - napF b2208 0.37 +1.1
2,301,256 - napF b2208 0.47 +0.5
2,301,257 - napF b2208 0.47 +0.5
2,301,286 + napF b2208 0.53 +0.2
2,301,415 - napF b2208 0.79 -0.1
2,301,496 - -0.0
2,301,620 + -0.6
2,301,874 + -0.2
2,302,083 + eco b2209 0.32 -0.0
2,302,294 + eco b2209 0.75 +0.8
2,302,310 - eco b2209 0.78 +0.5
2,302,522 + -0.1
2,302,748 + +0.2
2,302,763 + -0.9
2,302,763 + +0.8
2,302,773 + +0.2
2,303,223 - -0.0
2,303,225 + -0.3
2,303,226 + +0.5
2,303,244 + +0.8
2,303,346 - mqo b2210 0.13 +0.7
2,303,346 - mqo b2210 0.13 +0.1

Or see this region's nucleotide sequence