Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisG and hisD are separated by 5 nucleotides hisD and hisC overlap by 4 nucleotides
b2019: hisG - ATP phosphoribosyltransferase (NCBI), at 2,088,216 to 2,089,115
hisG
b2020: hisD - histidinol dehydrogenase (NCBI), at 2,089,121 to 2,090,425
hisD
b2021: hisC - histidinol-phosphate aminotransferase (NCBI), at 2,090,422 to 2,091,492
hisC
Position (kb)
2089
2090
2091 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2088.203 kb on - strand at 2088.203 kb on - strand at 2088.316 kb on + strand, within hisG at 2088.484 kb on - strand, within hisG at 2088.602 kb on + strand, within hisG at 2088.610 kb on - strand, within hisG at 2088.610 kb on - strand, within hisG at 2088.657 kb on - strand, within hisG at 2088.681 kb on - strand, within hisG at 2088.859 kb on + strand, within hisG at 2088.900 kb on + strand, within hisG at 2088.900 kb on + strand, within hisG at 2089.029 kb on + strand at 2089.104 kb on + strand at 2089.133 kb on - strand at 2089.155 kb on - strand at 2089.187 kb on - strand at 2089.234 kb on - strand at 2089.234 kb on - strand at 2089.267 kb on + strand, within hisD at 2089.326 kb on + strand, within hisD at 2089.419 kb on - strand, within hisD at 2089.504 kb on + strand, within hisD at 2089.605 kb on - strand, within hisD at 2089.755 kb on - strand, within hisD at 2089.776 kb on + strand, within hisD at 2089.844 kb on + strand, within hisD at 2089.929 kb on - strand, within hisD at 2089.929 kb on - strand, within hisD at 2090.018 kb on - strand, within hisD at 2090.018 kb on - strand, within hisD at 2090.075 kb on - strand, within hisD at 2090.124 kb on + strand, within hisD at 2090.124 kb on + strand, within hisD at 2090.132 kb on - strand, within hisD at 2090.300 kb on - strand at 2090.337 kb on - strand at 2090.419 kb on + strand at 2090.721 kb on - strand, within hisC at 2090.808 kb on - strand, within hisC at 2090.932 kb on + strand, within hisC at 2090.932 kb on + strand, within hisC at 2090.938 kb on + strand, within hisC at 2091.102 kb on + strand, within hisC at 2091.202 kb on - strand, within hisC at 2091.202 kb on - strand, within hisC at 2091.274 kb on + strand, within hisC at 2091.351 kb on + strand, within hisC
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 2,088,203 - +0.1 2,088,203 - -0.0 2,088,316 + hisG b2019 0.11 -0.3 2,088,484 - hisG b2019 0.30 -0.6 2,088,602 + hisG b2019 0.43 -0.1 2,088,610 - hisG b2019 0.44 +0.0 2,088,610 - hisG b2019 0.44 -0.2 2,088,657 - hisG b2019 0.49 +0.3 2,088,681 - hisG b2019 0.52 -0.2 2,088,859 + hisG b2019 0.71 +0.6 2,088,900 + hisG b2019 0.76 -1.7 2,088,900 + hisG b2019 0.76 -0.5 2,089,029 + -0.7 2,089,104 + +0.2 2,089,133 - -0.2 2,089,155 - -0.4 2,089,187 - -0.1 2,089,234 - -0.7 2,089,234 - -0.7 2,089,267 + hisD b2020 0.11 -3.2 2,089,326 + hisD b2020 0.16 -0.8 2,089,419 - hisD b2020 0.23 +0.3 2,089,504 + hisD b2020 0.29 -2.7 2,089,605 - hisD b2020 0.37 +0.1 2,089,755 - hisD b2020 0.49 -0.4 2,089,776 + hisD b2020 0.50 -1.7 2,089,844 + hisD b2020 0.55 -1.2 2,089,929 - hisD b2020 0.62 -0.4 2,089,929 - hisD b2020 0.62 -1.2 2,090,018 - hisD b2020 0.69 +0.7 2,090,018 - hisD b2020 0.69 -0.6 2,090,075 - hisD b2020 0.73 -0.3 2,090,124 + hisD b2020 0.77 -1.3 2,090,124 + hisD b2020 0.77 -1.3 2,090,132 - hisD b2020 0.77 -0.9 2,090,300 - +0.1 2,090,337 - -0.8 2,090,419 + +1.1 2,090,721 - hisC b2021 0.28 +1.3 2,090,808 - hisC b2021 0.36 +0.3 2,090,932 + hisC b2021 0.48 -0.4 2,090,932 + hisC b2021 0.48 -0.7 2,090,938 + hisC b2021 0.48 -0.5 2,091,102 + hisC b2021 0.63 -1.5 2,091,202 - hisC b2021 0.73 +0.2 2,091,202 - hisC b2021 0.73 +0.9 2,091,274 + hisC b2021 0.80 -1.5 2,091,351 + hisC b2021 0.87 -0.5
Or see this region's nucleotide sequence