Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yebB and ruvC are separated by 1 nucleotides ruvC and yebC are separated by 34 nucleotides yebC and nudB are separated by 28 nucleotides nudB and aspS are separated by 117 nucleotides
b1862: yebB - orf, hypothetical protein (VIMSS), at 1,944,275 to 1,944,877
yebB
b1863: ruvC - Holliday junction resolvase (NCBI), at 1,944,879 to 1,945,400
ruvC
b1864: yebC - hypothetical protein (NCBI), at 1,945,435 to 1,946,175
yebC
b1865: nudB - dATP pyrophosphohydrolase (NCBI), at 1,946,204 to 1,946,656
nudB
b1866: aspS - aspartyl-tRNA synthetase (NCBI), at 1,946,774 to 1,948,546
aspS
Position (kb)
1945
1946
1947 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1944.511 kb on - strand, within yebB at 1944.560 kb on - strand, within yebB at 1944.582 kb on + strand, within yebB at 1944.622 kb on + strand, within yebB at 1944.625 kb on - strand, within yebB at 1944.625 kb on - strand, within yebB at 1944.625 kb on - strand, within yebB at 1944.669 kb on - strand, within yebB at 1944.674 kb on - strand, within yebB at 1944.723 kb on - strand, within yebB at 1944.818 kb on + strand at 1944.818 kb on + strand at 1944.826 kb on - strand at 1944.826 kb on - strand at 1944.829 kb on + strand at 1944.846 kb on - strand at 1944.885 kb on - strand at 1944.885 kb on - strand at 1944.895 kb on - strand at 1944.908 kb on + strand at 1944.916 kb on - strand at 1945.037 kb on - strand, within ruvC at 1945.078 kb on - strand, within ruvC at 1945.219 kb on + strand, within ruvC at 1945.255 kb on - strand, within ruvC at 1945.260 kb on - strand, within ruvC at 1945.307 kb on + strand, within ruvC at 1945.323 kb on - strand, within ruvC at 1945.396 kb on - strand at 1945.455 kb on + strand at 1945.524 kb on - strand, within yebC at 1945.557 kb on - strand, within yebC at 1945.557 kb on - strand, within yebC at 1945.623 kb on - strand, within yebC at 1945.623 kb on - strand, within yebC at 1945.640 kb on - strand, within yebC at 1945.640 kb on - strand, within yebC at 1945.652 kb on + strand, within yebC at 1945.663 kb on - strand, within yebC at 1945.726 kb on - strand, within yebC at 1945.975 kb on + strand, within yebC at 1945.975 kb on + strand, within yebC at 1945.997 kb on + strand, within yebC at 1946.123 kb on - strand at 1946.247 kb on + strand at 1946.247 kb on + strand at 1946.435 kb on + strand, within nudB at 1946.471 kb on - strand, within nudB at 1946.471 kb on - strand, within nudB at 1946.636 kb on + strand at 1946.644 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 1,944,511 - yebB b1862 0.39 +0.2 1,944,560 - yebB b1862 0.47 +0.2 1,944,582 + yebB b1862 0.51 +2.2 1,944,622 + yebB b1862 0.58 +0.2 1,944,625 - yebB b1862 0.58 +0.2 1,944,625 - yebB b1862 0.58 +0.2 1,944,625 - yebB b1862 0.58 +0.2 1,944,669 - yebB b1862 0.65 +0.8 1,944,674 - yebB b1862 0.66 +0.2 1,944,723 - yebB b1862 0.74 +0.2 1,944,818 + -0.5 1,944,818 + +0.2 1,944,826 - +2.0 1,944,826 - +0.2 1,944,829 + +1.3 1,944,846 - -0.2 1,944,885 - +0.1 1,944,885 - +0.2 1,944,895 - -0.0 1,944,908 + +0.2 1,944,916 - +0.1 1,945,037 - ruvC b1863 0.30 +0.2 1,945,078 - ruvC b1863 0.38 -0.4 1,945,219 + ruvC b1863 0.65 -0.4 1,945,255 - ruvC b1863 0.72 -1.4 1,945,260 - ruvC b1863 0.73 -0.4 1,945,307 + ruvC b1863 0.82 +1.2 1,945,323 - ruvC b1863 0.85 -0.4 1,945,396 - +0.2 1,945,455 + -0.5 1,945,524 - yebC b1864 0.12 -0.9 1,945,557 - yebC b1864 0.16 -0.4 1,945,557 - yebC b1864 0.16 -0.5 1,945,623 - yebC b1864 0.25 -0.9 1,945,623 - yebC b1864 0.25 -2.7 1,945,640 - yebC b1864 0.28 +0.4 1,945,640 - yebC b1864 0.28 +0.5 1,945,652 + yebC b1864 0.29 -1.2 1,945,663 - yebC b1864 0.31 -0.8 1,945,726 - yebC b1864 0.39 -1.1 1,945,975 + yebC b1864 0.73 -1.1 1,945,975 + yebC b1864 0.73 +1.2 1,945,997 + yebC b1864 0.76 -0.7 1,946,123 - -0.6 1,946,247 + -0.6 1,946,247 + -1.4 1,946,435 + nudB b1865 0.51 +0.2 1,946,471 - nudB b1865 0.59 -0.9 1,946,471 - nudB b1865 0.59 -4.1 1,946,636 + -0.2 1,946,644 - -2.1
Or see this region's nucleotide sequence