Strain Fitness in Escherichia coli BW25113 around b1824

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrrmA and cspC are separated by 165 nucleotidescspC and yobF are separated by 12 nucleotidesyobF and yebO are separated by 669 nucleotides b1822: rrmA - 23S rRNA m1G745 methyltransferase (NCBI), at 1,904,275 to 1,905,084 rrmA b1823: cspC - stress protein, member of the CspA-family (NCBI), at 1,905,250 to 1,905,459 cspC b1824: yobF - hypothetical protein (NCBI), at 1,905,472 to 1,905,615 yobF b1825: yebO - hypothetical protein (NCBI), at 1,906,285 to 1,906,572 yebO Position (kb) 1905 1906Strain fitness (log2 ratio) -1 0 1 2at 1904.603 kb on + strand, within rrmAat 1904.603 kb on + strand, within rrmAat 1904.691 kb on + strand, within rrmAat 1904.897 kb on - strand, within rrmAat 1904.988 kb on - strand, within rrmAat 1905.238 kb on - strandat 1905.238 kb on - strandat 1905.348 kb on + strand, within cspCat 1905.364 kb on - strand, within cspCat 1905.432 kb on + strand, within cspCat 1905.432 kb on + strand, within cspCat 1905.534 kb on - strand, within yobFat 1905.534 kb on - strand, within yobFat 1905.536 kb on + strand, within yobFat 1905.562 kb on + strand, within yobFat 1905.562 kb on + strand, within yobFat 1905.613 kb on + strandat 1905.619 kb on - strandat 1905.629 kb on - strandat 1905.647 kb on + strandat 1905.751 kb on - strandat 1905.751 kb on - strandat 1905.771 kb on + strandat 1905.782 kb on + strandat 1905.879 kb on - strandat 1905.881 kb on - strandat 1906.101 kb on - strandat 1906.214 kb on - strandat 1906.230 kb on - strandat 1906.245 kb on - strandat 1906.248 kb on - strandat 1906.250 kb on - strandat 1906.256 kb on - strandat 1906.256 kb on - strandat 1906.300 kb on - strandat 1906.300 kb on - strandat 1906.588 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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1,904,603 + rrmA b1822 0.40 -0.1
1,904,603 + rrmA b1822 0.40 +0.6
1,904,691 + rrmA b1822 0.51 -0.6
1,904,897 - rrmA b1822 0.77 -1.0
1,904,988 - rrmA b1822 0.88 -0.2
1,905,238 - -0.2
1,905,238 - +0.0
1,905,348 + cspC b1823 0.47 +1.2
1,905,364 - cspC b1823 0.54 +0.6
1,905,432 + cspC b1823 0.87 +1.2
1,905,432 + cspC b1823 0.87 -0.4
1,905,534 - yobF b1824 0.43 +0.6
1,905,534 - yobF b1824 0.43 +0.5
1,905,536 + yobF b1824 0.44 -0.8
1,905,562 + yobF b1824 0.62 -0.6
1,905,562 + yobF b1824 0.62 +1.9
1,905,613 + +0.2
1,905,619 - +0.3
1,905,629 - -0.3
1,905,647 + +1.0
1,905,751 - +0.4
1,905,751 - -1.1
1,905,771 + +0.2
1,905,782 + -0.3
1,905,879 - +0.1
1,905,881 - +0.2
1,906,101 - -0.8
1,906,214 - -1.4
1,906,230 - -1.1
1,906,245 - +0.2
1,906,248 - -1.3
1,906,250 - -0.3
1,906,256 - +0.3
1,906,256 - +0.8
1,906,300 - -0.4
1,906,300 - +0.2
1,906,588 - -1.2

Or see this region's nucleotide sequence