Strain Fitness in Escherichia coli BW25113 around b1588

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntynfE and ynfF are separated by 60 nucleotidesynfF and ynfG are separated by 10 nucleotidesynfG and ynfH are separated by 1 nucleotides b1587: ynfE - oxidoreductase subunit (NCBI), at 1,656,093 to 1,658,519 ynfE b1588: ynfF - oxidoreductase subunit (RefSeq), at 1,658,580 to 1,661,003 ynfF b1589: ynfG - oxidoreductase, Fe-S subunit (NCBI), at 1,661,014 to 1,661,631 ynfG b1590: ynfH - oxidoreductase, membrane subunit (NCBI), at 1,661,633 to 1,662,487 ynfH Position (kb) 1658 1659 1660 1661 1662Strain fitness (log2 ratio) -1 0 1 2at 1657.604 kb on + strand, within ynfEat 1657.707 kb on - strand, within ynfEat 1657.762 kb on + strand, within ynfEat 1657.892 kb on + strand, within ynfEat 1658.038 kb on - strand, within ynfEat 1658.056 kb on + strand, within ynfEat 1658.249 kb on + strand, within ynfEat 1658.251 kb on - strand, within ynfEat 1658.251 kb on - strand, within ynfEat 1658.314 kb on + strandat 1658.488 kb on + strandat 1658.579 kb on + strandat 1658.579 kb on + strandat 1658.750 kb on + strandat 1658.750 kb on + strandat 1658.992 kb on + strand, within ynfFat 1659.171 kb on - strand, within ynfFat 1659.174 kb on - strand, within ynfFat 1659.176 kb on - strand, within ynfFat 1659.176 kb on - strand, within ynfFat 1659.475 kb on - strand, within ynfFat 1660.102 kb on + strand, within ynfFat 1660.208 kb on + strand, within ynfFat 1660.366 kb on - strand, within ynfFat 1660.378 kb on + strand, within ynfFat 1660.766 kb on + strandat 1660.766 kb on + strandat 1660.779 kb on - strandat 1660.779 kb on - strandat 1660.790 kb on - strandat 1661.057 kb on - strandat 1661.346 kb on - strand, within ynfGat 1661.351 kb on + strand, within ynfGat 1661.351 kb on + strand, within ynfGat 1661.364 kb on - strand, within ynfGat 1661.755 kb on + strand, within ynfHat 1661.755 kb on + strand, within ynfHat 1661.782 kb on - strand, within ynfHat 1661.851 kb on - strand, within ynfH

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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1,657,604 + ynfE b1587 0.62 +0.7
1,657,707 - ynfE b1587 0.67 -0.9
1,657,762 + ynfE b1587 0.69 +0.2
1,657,892 + ynfE b1587 0.74 +0.1
1,658,038 - ynfE b1587 0.80 +0.4
1,658,056 + ynfE b1587 0.81 -0.0
1,658,249 + ynfE b1587 0.89 +0.2
1,658,251 - ynfE b1587 0.89 +0.6
1,658,251 - ynfE b1587 0.89 -1.1
1,658,314 + +0.3
1,658,488 + +0.5
1,658,579 + +0.1
1,658,579 + +0.9
1,658,750 + -0.1
1,658,750 + +0.3
1,658,992 + ynfF b1588 0.17 +0.9
1,659,171 - ynfF b1588 0.24 +1.2
1,659,174 - ynfF b1588 0.25 +0.3
1,659,176 - ynfF b1588 0.25 -0.7
1,659,176 - ynfF b1588 0.25 +0.3
1,659,475 - ynfF b1588 0.37 +0.2
1,660,102 + ynfF b1588 0.63 +0.1
1,660,208 + ynfF b1588 0.67 -1.1
1,660,366 - ynfF b1588 0.74 +2.4
1,660,378 + ynfF b1588 0.74 -1.2
1,660,766 + +0.5
1,660,766 + +1.0
1,660,779 - +0.5
1,660,779 - +0.7
1,660,790 - +0.1
1,661,057 - +1.0
1,661,346 - ynfG b1589 0.54 +0.0
1,661,351 + ynfG b1589 0.55 -0.4
1,661,351 + ynfG b1589 0.55 +0.3
1,661,364 - ynfG b1589 0.57 -0.1
1,661,755 + ynfH b1590 0.14 +0.1
1,661,755 + ynfH b1590 0.14 +0.7
1,661,782 - ynfH b1590 0.17 -0.7
1,661,851 - ynfH b1590 0.25 +0.2

Or see this region's nucleotide sequence